USP13

Gene Symbol USP13
Entrez Gene 8975
Alt Symbol ISOT3, IsoT-3
Species Human
Gene Type protein-coding
Description ubiquitin specific peptidase 13 (isopeptidase T-3)
Other Description deubiquitinating enzyme 13|ubiquitin carboxyl-terminal hydrolase 13|ubiquitin specific protease 13 (isopeptidase T-3)|ubiquitin thioesterase 13|ubiquitin thiolesterase 13|ubiquitin-specific-processing protease 13
Swissprots A8K2S3 D3DNS2 B4DYF3 Q96B25 Q92995
Accessions EAW78383 EAW78384 Q92995 AK091484 AK290338 BAF83027 AK302404 BAG63715 AM393315 CAL38193 AM393392 CAL38270 AM393610 CAL38486 AM393780 CAL38655 BC016146 AAH16146 BC049199 BC062766 DQ892472 ABM83398 DQ895684 ABM86610 HM138083 ADM34989 U75362 AAC63405 XM_011513269 XP_011511571 NM_003940 NP_003931
Function Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak
Tissue Specificity Highly expressed in ovary and testes.

USP13 (D4P3M) Rabbit mAb - 12577 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4P3M
Western blot analysis of extracts from various cell lines using USP13 (D4P3M) Rabbit mAb.

USP13 (B-9) - sc-514416 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP13 (B-9): sc-514416. Western blot analysis of USP13 expression in non-transfected 293T: sc-117752 (A), human USP13 transfected 293T: sc-174342 (B), SW480 (C), SH-SY5Y (D) and Hep G2 (E) whole cell lysates.

USP13 (D-11) - sc-390316 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP13 (D-11): sc-390316. Western blot analysis of USP13 expression in non-transfected: sc-117752 (A) and human USP13 transfected: sc-174342 (B) 293T whole cell lysates.

USP13 (E-11) - sc-514442 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP13 (E-11): sc-514442. Western blot analysis of USP13 expression in SW480 (A), SH-SY5Y (B) and Hep G2 (C) whole cell lysates.

USP13 (H-125) - sc-366878 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
USP13 (H-125): sc-366878. Western blot analysis of USP13 expression in non-transfected: sc-117752 (A) and human USP13 transfected: sc-174342 (B) 293T whole cell lysates and mouse brain tissue extract (C).

USP13 (N-18) - sc-82075 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
USP13 (N-18): sc-82075. Western blot analysis of USP13 expression in SW480 whole cell lysate.

USP13 (S-18) - sc-82077 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
USP13 (S-18): sc-82077. Western blot analysis of USP13 expression in non-transfected: sc-117752 (A) and human USP13 transfected: sc-174342 (B) 293T whole cell lysates.

Anti-USP13 antibody - ab71734 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-USP13 antibody (ab71734) at 1/100 dilutionLane 1 : HeLa cell lysate Lane 2 : Mouse brain tissue lysate Lysates/proteins at 12.5 µg per lane.

Anti-USP13 antibody - ab99423 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP13 with ab99423 at 1/5000 (0.2µg/ml). Detection: DAB.

Anti-USP13 antibody [5B9] - ab117873 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5B9
All lanes : Anti-USP13 antibody [5B9] (ab117873) at 1/2000 dilutionLane 1 : HEK293 cells transfected with pCMV6-ENTRY controlLane 2 : HEK293 cells transfected with pCMV6-ENTRY USP13Lysates/proteins at 5 µg per lane.

Anti-USP13 antibody [EPR4348] - ab109264 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4348
Overlay histogram showing SHSY-5Y cells stained with ab109264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109264, 1/9400 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-USP13 antibody - N-terminal - ab71738 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-USP13 antibody - N-terminal (ab71738) at 1/100 dilution + A375 cell lysate at 12.5 µg

Anti-USP13 Antibody - LS-C132832 from LifeSpan Bioscience

Host Rabbit
Clonality Monoclonal

Anti-USP13 Antibody (aa175-190) - LS-C148991 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (Internal, AP) - LS-C284158 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (aa161-210) - LS-C116947 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
USP13 antibody LS-C116947 Western blot of Placenta lysate.

Anti-USP13 Antibody (Internal, APC) - LS-C284160 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (Internal, Biotin) - LS-C284161 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (Internal, FITC) - LS-C284162 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (aa803-852) - LS-C200806 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from RAW264.7cells using USP13 antibody.

Anti-USP13 Antibody (N-Terminus, APC) - LS-C284164 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (Internal, Azide-free, HRP) - LS-C284163 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (N-Terminus, AP) - LS-C284165 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (N-Terminus, Biotin) - LS-C284166 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP13 Antibody (aa156-186) - LS-C99552 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Detection of USP13 in HeLa cells expressing exogenous USP13 by anti-USP13 antibody. (Data provided by Amy Chen, Burnham Institute for Medical Research)
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