Gene Symbol | BRCC3 |
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Entrez Gene | 79184 |
Alt Symbol | BRCC36, C6.1A, CXorf53 |
Species | Human |
Gene Type | protein-coding |
Description | BRCA1/BRCA2-containing complex, subunit 3 |
Other Description | BRCA1-A complex subunit BRCC36|BRCA1/BRCA2-containing complex subunit 36|BRISC complex subunit BRCC36|lys-63-specific deubiquitinase BRCC36 |
Swissprots | Q16107 A9Z1Y5 Q9BTZ6 B1B062 B4DQN7 A6QRF8 P46736 Q53YX5 A8MWH0 A6QRF9 A8MUX5 A9Z1Y0 |
Accessions | EAW72632 EAW72633 EAW72634 EAW72635 EAW72636 EAW72637 P46736 AK298886 BAG60999 AK299194 BAG61237 AK310286 AK313544 BAG36320 AM392829 CAL37707 AY438030 AAR30498 BC002999 AAH02999 BC006540 AAH06540 BP304352 BU902387 S68015 AAB29005 X64643 CAA45917 XM_005274751 XP_005274808 NM_001018055 NP_001018065 NM_001242640 NP_001229569 NM_024332 NP_077308 |
Function | Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. {ECO:0000269|PubMed:14636569, ECO:0000269|PubMed:16707425, ECO:0000269|PubMed:17525341, ECO:0000269|PubMed:1920 |
Subcellular Location | Nucleus {ECO:0000269|PubMed:18077395, ECO:0000269|PubMed:19202061, ECO:0000269|PubMed:19261748, ECO:0000269|PubMed:19261749}. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs). |
Tissue Specificity | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors. {ECO:0000269|PubMed:16707425}. |
BRCC3 siRNA (h) - sc-90932 from Santa Cruz Biotechnology
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BRCC3 shRNA Plasmid (h) - sc-90932-SH from Santa Cruz Biotechnology
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BRCC3 shRNA (h) Lentiviral Particles - sc-90932-V from Santa Cruz Biotechnology
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BRCC3 CRISPR/Cas9 KO Plasmid (h) - sc-407685 from Santa Cruz Biotechnology
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BRCC3 HDR Plasmid (h) - sc-407685-HDR from Santa Cruz Biotechnology
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BRCC3 Double Nickase Plasmid (h) - sc-407685-NIC from Santa Cruz Biotechnology
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BRCC3 Double Nickase Plasmid (h2) - sc-407685-NIC-2 from Santa Cruz Biotechnology
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BRCC3 CRISPR Activation Plasmid (h) - sc-407685-ACT from Santa Cruz Biotechnology
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BRCC3 CRISPR Activation Plasmid (h2) - sc-407685-ACT-2 from Santa Cruz Biotechnology
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BRCC3 Lentiviral Activation Particles (h) - sc-407685-LAC from Santa Cruz Biotechnology
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BRCC3 Lentiviral Activation Particles (h2) - sc-407685-LAC-2 from Santa Cruz Biotechnology
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DEPDC1B siRNA (h) - sc-91972 from Santa Cruz Biotechnology
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DEPDC1B shRNA Plasmid (h) - sc-91972-SH from Santa Cruz Biotechnology
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DEPDC1B shRNA (h) Lentiviral Particles - sc-91972-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU069041 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN4046 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND4046 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR10654 from Sigma-Aldrich
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BRCC3 siRNA (Human) - CRJ2353 from Cohesion Biosciences
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BRCC3 - MBS828095 from MyBioSource
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shRNA set against Human BRCC3 (NM_024332.3) - SHH247570 from Creative biogene
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Human BRCC3 siRNA - orb272854 from Biorbyt
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BRCC3 shRNA Plasmid (Human) - CHJ2353 from Cohesion Biosciences
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BRCC3 miRNA 3'UTR clone - MiUTR3H-02873 from Creative biogene
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BRCC3 miRNA 3'UTR clone - MiUTR3H-02874 from Creative biogene
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