LDLRAP1

Gene Symbol LDLRAP1
Entrez Gene 26119
Alt Symbol ARH, ARH1, ARH2, FHCB1, FHCB2
Species Human
Gene Type protein-coding
Description low density lipoprotein receptor adaptor protein 1
Other Description LDL receptor adaptor protein|autosomal recessive hypercholesterolemia protein|low density lipoprotein receptor adapter protein 1
Swissprots Q6TQS9 Q9UFI9 A2BHI5 Q8N2Y0 Q5SW96
Accessions AAQ90407 EAX07872 EAX07873 EAX07874 EAX07875 Q5SW96 AK024397 BAB14908 AK091219 BAG52309 AK124554 AK309850 AK309853 AL117654 CAB56030 BC029770 AAH29770 DA930479 HG491793 XM_006710559 XP_006710622 XM_006710560 XP_006710623 XM_006710561 XP_006710624 XM_011541209 XP_011539511 XM_011541210 XP_011539512 XM_011541211 XP_011539513 XM_011541212 XP_011539514 XR_426598 XR_946602 XR_946603 NM_015627 NP_056442
Function Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. {ECO:0000269|PubMed:15728179}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:12451172}.
Tissue Specificity Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.
Top Pathways Endocytosis

ARH (E-10) - sc-514263 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARH (E-10): sc-514263. Western blot analysis of ARH expression in HeLa nuclear extract (A) and Hep G2 (B) and U-87 MG (C) whole cell lysates.

ARH (F-11) - sc-514106 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARH (F-11): sc-514106. Western blot analysis of ARH expression in non-transfected 293T: sc-117752 (A), human ARH transfected 293T: sc-114378 (B), Hep G2 (D) and U-87 MG (E) whole cell lysates and HeLa nuclear extract (C).

ARH (H-119) - sc-292294 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ARH (H-119): sc-292294. Western blot analysis of ARH expression in non-transfected 293T: sc-117752 (A), human ARH transfected 293T: sc-114378 (B), NCI-H460 (C), Hep G2 (D) and U-87 MG (E) whole cell lysates.

ARH (T-19) - sc-26734 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ARH (T-19): sc-26734. Western blot analysis of ARH expression in non-transfected: sc-117752 (A) and mouse ARH transfected: sc-124988 (B) 293T whole cell lysates.

ARH (E-22) - sc-130692 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
ARH (E-22): sc-130692. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.

ARH (Q-13) - sc-100653 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
ARH (Q-13): sc-100653. Western blot analysis of ARH expression in 293T whole cell lysate.

Anti-ARH antibody - ab57261 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HEK293 cells stained with ab57261 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57261, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ARH antibody - ab127012 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-ARH antibody (ab127012) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg

Anti-ARH antibody - ab87728 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARH antibody (ab87728) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ARH antibody - ab5082 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-ARH antibody [2B2] - ab123920 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B2
All lanes : Anti-ARH antibody [2B2] (ab123920) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T cells transfected with pCMV6-ENTRY ARH cDNA Lysates/proteins at 5 µg per lane.

Anti-ARH antibody [EPR13115] - N-terminal - ab181043 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13115
Western blot analysis of ARH in immunoprecipitation pellets from K562. ab181043 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

Anti-ARH antibody [EPR13116] - C-terminal - ab179828 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13116
All lanes : Anti-ARH antibody [EPR13116] - C-terminal (ab179828) at 1/10000 dilutionLane 1 : Human fetal liver lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-ARH antibody - C-terminal - ab200117 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARH antibody - C-terminal (ab200117) at 1/1000 dilutionLane 1 : Mouse thymus total cell extractsLane 2 : Jurkat total cell extracts

Anti-ARH antibody - Carboxyterminal end - ab63155 from Abcam

Prices $304.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ARH antibody - Carboxyterminal end (ab63155) at 1/60 dilution + NCI-H460 cell line lysates at 35 µg

Anti-ARH / LDLRAP1 Antibody (aa35-84) - LS-C111631 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
LDLRAP1 antibody LS-C111631 Western blot of HepG2 cell lysate.

Anti-ARH / LDLRAP1 Antibody (aa11-42) - LS-C99830 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with LDLRAP1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-ARH / LDLRAP1 Antibody (aa2-14) - LS-C20125 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Staining (3 ug/ml) of Human Liver lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-ARH / LDLRAP1 Antibody (aa277-306) - LS-C99831 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LDLRAP1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-ARH / LDLRAP1 Antibody (N-Terminus, AP) - LS-C243734 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARH / LDLRAP1 Antibody (N-Terminus, Biotin) - LS-C243735 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARH / LDLRAP1 Antibody (N-Terminus, APC) - LS-C243736 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARH / LDLRAP1 Antibody (N-Terminus, FITC) - LS-C243737 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARH / LDLRAP1 Antibody (N-Terminus, PE) - LS-C243738 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARH / LDLRAP1 Antibody (clone 4G4-D5) IHC-plus™ - LS-B4523 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4G4-D5
Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
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