ZC3HC1

Gene Symbol ZC3HC1
Entrez Gene 51530
Alt Symbol HSPC216, NIPA
Species Human
Gene Type protein-coding
Description zinc finger, C3HC-type containing 1
Other Description hematopoietic stem/progenitor cell protein 216|nuclear interacting partner of anaplastic lymphoma kinase (ALK)|nuclear-interacting partner of ALK
Swissprots Q9HA34 Q8N330 Q86WB0 Q9NVX4 A6NH66 Q75MF4 Q96F75 Q75MF3 Q9P0R0
Accessions AAS07546 AAS07547 CAE89297 EAL24097 EAW83738 EAW83739 EAW83740 EAW83741 EAW83742 EAW83743 Q86WB0 AF151050 AAF36136 AJ537494 CAD61161 AK001317 BAA91619 AK022373 BAB14024 AM393415 CAL38293 BC000190 AAH00190 BC011551 AAH11551 BC028917 AAH28917 BF745935 BI830062 BM013594 BX490781 DA066102 XM_005250403 XP_005250460 XM_011516288 XP_011514590 XM_011516289 XP_011514591 XM_011516290 XP_011514592 NM_001282190 NP_001269119 NM_001282191 NP_001269120 NM_016478 NP_057562
Function Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. {ECO:0000269|PubMed:16009132}.
Subcellular Location Nucleus {ECO:0000269|PubMed:12748172}.
Tissue Specificity Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes. {ECO:0000269|PubMed:12748172}.

NIPA Antibody - 4497 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, either untreated (asynchronous) or treated with nocodazole or hydroxyurea, using NIPA Antibody.

NIPA (A-12) - sc-514368 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NIPA (A-12): sc-514368. Western blot analysis of NIPA expression in HeLa (A), WI-38 (B) and U-251-MG (C) whole cell lysates.

NIPA (B-10) - sc-365058 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NIPA (B-10): sc-365058. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear membrane localization.

NIPA (C-14) - sc-48603 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NIPA (C-14): sc-48603. Western blot analysis of NIPA expression in HeLa (A) and WI 38 (B) whole cell lysates.

NIPA (H-300) - sc-134601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NIPA (H-300): sc-134601. Western blot analysis of NIPA expression in HeLa (A) and WI 38 (B) whole cell lysates and mouse brain (C) and mouse liver (D) tissue extracts.

NIPA (N-16) - sc-48605 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NIPA (N-16): sc-48605. Western blot analysis of NIPA expression in HeLa (A) and WI 38 (B) whole cell lysates.

Anti-NIPA (phospho S354) antibody - ab63557 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of NIPA staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63557, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NIPA antibody - ab63375 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of NIPA staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63375, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NIPA antibody - ab176847 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NIPA antibody (ab176847) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-NIPA antibody - ab169486 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-NIPA antibody (ab169486) at 1/500 dilutionLane 1 : NIPA transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-ZC3HC1 / NIPA Antibody (aa320-369) IHC-plus™ - LS-B9866 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ZC3HC1 / NIPA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9866 concentration 20 ug/ml.

Anti-ZC3HC1 / NIPA Antibody (aa300-350) - LS-C289649 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ZC3HC1 / NIPA Antibody (aa1-50) - LS-C289647 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ZC3HC1 / NIPA Antibody (Ser350) - LS-C176682 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of NIPA (S350) pAb in extracts from COS7 cells.

Anti-ZC3HC1 / NIPA Antibody (phospho-Ser354) - LS-C199324 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from COS7 cells treated with HU 2nM 24h, using NIPA (Phospho-Ser354) Antibody. The lane on the right was incubated with synthetic peptide.

Anti-ZC3HC1 / NIPA Antibody - LS-C334644 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of HeLa cell line, using ZC3HC1 antibody.

NIPA Antibody - NBP1-82564 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NIPA Antibody [NBP1-82564] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

NIPA Antibody - NBP1-82565 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NIPA Antibody [NBP1-82565] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

NIPA Antibody - NBP1-19525 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: NIPA Antibody [NBP1-19525] - extracts from COS7 cells.

NIPA Antibody - H00051530-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: NIPA Antibody [H00051530-B01] - analysis of ZC3HC1 expression in transfected 293T cell line by ZC3HC1 MaxPab polyclonal antibody (B01). Lane 1: ZC3HC1 transfected lysate (23.20 KDa). Lane 2: Non-transfected lysate.

NIPA Polyclonal Antibody - bs-8363R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NIPA(Ser354) Polyclonal Antibody - bs-8364R from Bioss

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIPA antibody produced in rabbit - SAB4502133 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-NIPA : <B>Cat. No. SAB4502133</B>: Immunofluorescence of HeLa cells using Anti-NIPA antibody. Cells on the left were treated with the Anti-NIPA antibody. Cells on the right (negative control) were treated with both Anti-NIPA antibody and the synthesized immunogen peptide.

Anti-ZC3HC1 antibody produced in rabbit - HPA019089 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ZC3HC1: <B>Cat. No. HPA019089</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human gall bladder shows nuclear and nuclear membrane positivity in glandular cells.

Anti-phospho-NIPA (pSer354) antibody produced in rabbit - SAB4504286 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-phoshpho-NIPA (pSer<SUP>354</SUP>): <B>Cat. No. SAB4504286</B>: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
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