PNKP

Gene Symbol PNKP
Entrez Gene 11284
Alt Symbol AOA4, EIEE10, MCSZ, PNK
Species Human
Gene Type protein-coding
Description polynucleotide kinase 3'-phosphatase
Other Description DNA 5'-kinase/3'-phosphatase|Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)|bifunctional polynucleotide phosphatase/kinase
Swissprots Q9BUL2 Q96T60 Q9UKU8 Q9P1V2 Q9UNI0 Q9UNF8
Accessions AAF44716 AAK57340 AAM82170 EAW52565 EAW52566 EAW52567 Q96T60 AA640353 AF120499 AAD47379 AF125807 AAD50639 AF126486 AAD51135 BC002519 AAH02519 BC009339 BC013034 BC033822 AAH33822 BU541073 DQ890806 ABM81732 DQ893961 ABM84887 NM_007254 NP_009185
Function Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. {ECO:0000269|PubMed:10446192}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10446193}.
Tissue Specificity Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193). {ECO:0000269|PubMed:10446192, ECO:0000269|PubMed:10446193}.

Phospho-PNK1 (Ser114/Thr118) Antibody - 3522 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of 293 and HeLa cells, either untreated or treated with UV radiation, using Phospho-PNK1 (Ser114/Thr118) Antibody (upper). β-actin Antibody #4967 (lower) was used to control for loading.

PNK (A-4) - sc-271525 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNK (A-4): sc-271525. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (B-4) - sc-166188 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNK (B-4): sc-166188. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (B-5) - sc-365724 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNK (B-5): sc-365724. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (C-11) - sc-166080 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNK (C-11): sc-166080. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (E-9) - sc-271135 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNK (E-9): sc-271135. Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear and cytoplasmic staining of glandular cells.

PNK (F-3) - sc-271505 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNK (F-3): sc-271505. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (F-5) - sc-166153 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNK (F-5): sc-166153. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (H-300) - sc-67036 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PNK (H-300): sc-67036. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (K-20) - sc-34302 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PNK (K-20): sc-34302. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (C-20) - sc-34303 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PNK (C-20): sc-34303. Western blot analysis of PNK expression in non-transfected: sc-117752 (A) and human PNK transfected: sc-115119 (B) 293T whole cell lysates.

PNK (V-20) - sc-34301 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PNK (V-20): sc-34301. Western blot analysis of PNK expression in HeLa (A), PC-3 (B) and Jurkat (C) nuclear extracts and rat testis tissue extract (D).

Anti-PNK antibody - ab70739 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PNK with ab70739 at 1/1000 (1µg/ml). Detection: DAB.

Anti-PNK antibody - ab151418 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PNK antibody (ab151418) at 1/2000 dilution + A549 whole cell lysate at 30 µg

Anti-PNK antibody - ab3817 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-PNK antibody - ab84374 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84374 at 1/250, staining human PNK in a section of human ovarian carcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PNK antibody - ab168473 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-PNK antibody (ab168473) at 1 µg/mlLane 1 : PNK transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-PNK antibody - ab4191 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1:  Y190 yeast cell lysate, 10 µg.Lane 2:  Y190 yeast cell lysate + human PNK (Gal DNA BP), 10 µg.Lane 3:  EM9 XH chinese hamster ovary cell lysate, 10 µg.   Lane 4:  EM9 XH chinese hamster ovary cell lysate + human PNK, 10 µg.Lane 5:  HeLa cell lysate, 10 µg.  ab4191 was incubated for 1-2 hours in TBST at a dilution of 1/1000.

Anti-PNK antibody - ab70740 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PNK antibody [EPR11934(B)] - ab170954 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11934(B)
ab170954 showing +ve staining in Human testis tissue.

Anti-PNK antibody [EPR11935] - C-terminal - ab181107 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11935
All lanes : Anti-PNK antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilutionLane 1 : Jurkat cell lysateLane 2 : Human testis lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PNKP Antibody - LS-C334883 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using PNKP antibody.

Anti-PNKP Antibody (aa253-418) - LS-C179167 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PNKP Antibody (aa250-300) - LS-C286324 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PNK1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PNK1 used at a dilution of 1:250. Detection: DAB.

Anti-PNKP Antibody (aa100-150) - LS-C287168 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PNK1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PNK1 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
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