MED21

Gene Symbol MED21
Entrez Gene 9412
Alt Symbol SRB7, SURB7, hSrb7
Species Human
Gene Type protein-coding
Description mediator complex subunit 21
Other Description RNA polymerase II holoenzyme component SRB7|RNAPII complex component SRB7|SRB7 suppressor of RNA polymerase B homolog|mediator of RNA polymerase II transcription subunit 21
Swissprots Q92811 Q6IB05 Q13503 B2R4I3
Accessions EAW96544 EAW96545 EAW96546 EAW96547 Q13503 AK309064 AK311838 BAG34780 BC008380 AAH08380 CR456999 CAG33280 DA420057 DB024509 EU446930 ABZ92459 HY075642 U46837 AAA98511 U52960 AAB06944 NM_001271811 NP_001258740 NM_004264 NP_004255 NR_073463 NR_073464
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:15249124, ECO:0000269|PubMed:9660976}.
Subcellular Location Nucleus {ECO:0000305}.

Srb7 (H-104) - sc-367660 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Srb7 (I-20) - sc-12459 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Srb7 (V-19) - sc-9439 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Srb7 (31-C) - sc-101186 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Srb7 (31-C): sc-101186. Western blot analysis of Srb7 expression in HeLa whole cell lysate.

Anti-Surb7 antibody - ab56503 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab56503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56503, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Surb7 antibody - ab111069 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111069 at 1/50 dilution staining Surb7 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

Anti-MED21 Antibody - LS-C91853 from LifeSpan Bioscience

Prices $475.00
Sizes 150 µg
Host Mouse
Clonality Monoclonal

Anti-MED21 Antibody (aa1-145, Azide-free) - LS-C328807 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-MED21 Antibody (C-Terminus) - LS-C146262 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MED21 / SURB7 antibody LS-C146262 Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.

Anti-MED21 Antibody (aa19-68) - LS-C145912 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MED21 / SURB7 antibody LS-C145912 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.

Anti-MED21 Antibody (aa74-123) - LS-C80167 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.

Anti-MED21 Antibody (clone 3E9) - LS-C133587 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9
Immunofluorescence of monoclonal antibody to SURB7 on HeLa cell. [antibody concentration 10 ug/ml]

Anti-MED21 Antibody (aa71-120) IHC-plus™ - LS-B8410 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MED21 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8410 dilution 5 ug/ml.

MED21 Antibody (3E9) - H00009412-M08 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9
Western Blot: MED21 Antibody (3E9) [H00009412-M08] - Analysis of SURB7 expression in HeLa (Cat # L013V1).

MED21 Antibody - NBP1-87006 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MED21 Antibody (2D9) - H00009412-M10 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D9
Western Blot: MED21 Antibody (2D9) [H00009412-M10] - Analysis of SURB7 expression in HeLa (Cat # L013V1).

MED21 Antibody (6B6) - H00009412-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6B6
Western Blot: MED21 Antibody (6B6) [H00009412-M03] - Analysis of SURB7 expression in HeLa (Cat # L013V1).

MED21 Antibody (5A6) - H00009412-M04 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5A6
Western Blot: MED21 Antibody (5A6) [H00009412-M04] - Analysis of SURB7 expression in HeLa (Cat # L013V1).

MED21 Antibody - H00009412-B01 from Novus Biologicals

Host Mouse
Clonality Polyclonal
Western Blot: MED21 Antibody [H00009412-B01] - analysis of SURB7 expression in transfected 293T cell line by SURB7 MaxPab polyclonal antibody (B01). Lane 1: SURB7 transfected lysate (15.60 KDa). Lane 2: Non-transfected lysate.

Anti-MED21 antibody produced in rabbit - SAB4503634 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED21: <B>Cat. No. SAB4503634</B>: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-MED21 antibody. Cells on the left were treated with the Anti-MED21 antibody. Cells on the right (negative control) were treated with both Anti-MED21 antibody and the synthesized immunogen peptide.

Anti-MED21 antibody produced in rabbit - HPA017177 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED21: <B>Cat. No. HPA017177</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in glomerular cells.

Monoclonal Anti-SURB7 antibody produced in mouse - WH0009412M4 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5A6
<B>Immunoblotting</B><BR/>Monoclonal Anti-SURB7, <B>Cat. No. WH0009412M4</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~15.5 kDa using HeLa lysate.

ANTI-SURB7 antibody produced in mouse - SAB1411811 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D9
<B>Immunofluorescence</B><BR/>Monoclonal Anti-SURB7: <B>Cat. No. SAB1411811</B>: Antibody concentration: 10 μg/mL using HeLa cell.

Monoclonal Anti-SURB7 antibody produced in mouse - SAB1404715 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9
<B>Immunohistochemistry</B><BR/>Monoclonal Anti-SURB7: <B>Cat. No. SAB1404715</B>: Antibody concentration: 1.2 μg/mL using human tonsil tissue.

ANTI-SURB7 antibody produced in mouse - SAB1411882 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6B6
<B>Immunofluorescence</B><BR/>Monoclonal Anti-SURB7: <B>Cat. No. SAB1411882</B>: Antibody concentration: 10 μg/mL using HeLa cell.
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