WWP1

Gene Symbol WWP1
Entrez Gene 11059
Alt Symbol AIP5, Tiul1, hSDRP1
Species Human
Gene Type protein-coding
Description WW domain containing E3 ubiquitin protein ligase 1
Other Description NEDD4-like E3 ubiquitin-protein ligase WWP1|Nedd-4-like ubiquitin-protein ligase|TGIF-interacting ubiquitin ligase 1|WW domain-containing protein 1|atrophin-1 interacting protein 5|atrophin-1-interacting protein 5
Swissprots O00307 Q5YLC1 Q96BP4 Q9H0M0
Accessions EAW91634 EAW91635 EAW91636 EAW91637 Q9H0M0 AK292800 BAF85489 AK310683 AL050082 AL136739 CAB66673 AW137654 AY043361 AAK94668 AY345857 AAQ22764 BC015380 AAH15380 BC036065 AAH36065 BM664090 EU832274 ACE87531 EU832359 ACE86844 U96113 AAC51324 XM_005250760 XP_005250817 XM_005250761 XP_005250818 XM_011516802 XP_011515104 NM_007013 NP_008944
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. {ECO:0000269|PubMed:12535537, ECO:0000269|PubMed:15221015, ECO:0000269|PubMed:15359284}.
Subcellular Location Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Nucleus {ECO:0000250}.
Tissue Specificity Detected in heart, placenta, pancreas, kidney, liver, skeletal muscle, bone marrow, fetal brain, and at much lower levels in adult brain and lung. Isoform 1 and isoform 5 predominate in all tissues tested, except in testis and bone marrow, where isoform 5 is expressed at much higher levels than isoform 1. {ECO:0000269|PubMed:11779188, ECO:0000269|PubMed:9647693}.
Top Pathways Ubiquitin mediated proteolysis, Endocytosis

AIP5 (F-3) - sc-390897 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AIP5 (F-3): sc-390897. Western blot analysis of AIP5 expression in SJRH30 (A) and K-562 (B) whole cell lysates.

AIP5 (N-20) - sc-11892 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AIP5 (K-20) - sc-11894 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AIP5 (K-20): sc-11894. Western blot analysis of AIP5 expression in K-562 (A), SJRH30 (B) and JAR (C) whole cell lysates.

AIP5 (P-16) - sc-11893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AIP5 (P-16): sc-11893. Western blot analysis of AIP5 expression in HeLa (A), HEK293 (B) and PC-12 (C) whole cell lysates.

AIP5 (H-260) - sc-30053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AIP5 (H-260): sc-30053. Western blot analysis of AIP5 expression in A-431 whole cell lysate.

AIP5 (5AA) - sc-100679 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
AIP5 (5AA): sc-100679. Western blot analysis of AIP5 expression in A-431 whole cell lysate.

Anti-WWP1 antibody - ab43791 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-WWP1 antibody (ab43791) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-WWP1 antibody - ab104440 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-WWP1 antibody (ab104440) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-WWP1 antibody - ab181821 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-WWP1 Antibody (aa300-350) - LS-C289655 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-WWP1 Antibody (aa200-250) - LS-C289654 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-WWP1 Antibody - LS-C170820 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-WWP1 Antibody (N-Terminus) - LS-C110755 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
WWP1 antibody LS-C110755 Western blot of 293T cell lysate.

Anti-WWP1 Antibody (clone 1A7) - LS-C105109 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell (antibody concentration 10 ug/ml).

Anti-WWP1 Antibody - LS-C333953 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of PC-3 cell line, using WWP1 antibody.

WWP1 Antibody (1A7) - H00011059-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
Western Blot: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody (M01A), clone 1A7.Lane 1: WWP1 transfected lysate(105.2 KDa).Lane 2: Non-transfected lysate.

WWP1 Antibody - NBP1-89179 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: WWP1 Antibody [NBP1-89179] - Staining of human cell line A-431 shows positivity in cytoplasm and golgi apparatus.

WWP1 Antibody - NBP1-49713 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: WWP1 Antibody [NBP1-49713] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WWP1 antibody NBP1-49713 used for IP at 3 ug/mg lysate. WWP1 was also immunoprecipitated by rabbit anti-WWP1 antibody NBP1-49712, which recognizes an upstream epitope. For blotting immunoprecipitated WWP1, NBP1-49712 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

WWP1 Antibody - NBP1-49712 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: WWP1 Antibody [NBP1-49712] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WWP1 antibody NBP1-49712 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WWP1 was also immunoprecipitated by rabbit anti-WWP1 antibody NBP1-49713, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

WWP1 Antibody - H00011059-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: WWP1 Antibody [H00011059-D01P] - Analysis of WWP1 expression in transfected 293T cell line by WWP1 polyclonal antibody.Lane 1: WWP1 transfected lysate(105.20 KDa).Lane 2: Non-transfected lysate.

WWP1 Antibody - H00011059-A01 from Novus Biologicals

Host Mouse
Clonality Polyclonal

WWP1 Antibody (1A7) - H00011059-M01A from Novus Biologicals

Prices $359.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 1A7
Western Blot: WWP1 Antibody (1A7) [H00011059-M01A] - Analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody (M01A), clone 1A7.Lane 1: WWP1 transfected lysate(105.2 KDa).Lane 2: Non-transfected lysate.

WWP1 Polyclonal Antibody - bs-6753R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-WWP1 antibody produced in mouse - WH0011059M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
<B>Immunoblotting</B><BR/>Monoclonal Anti-WWP1, <B>Cat. No. WH0011059M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~105.2 kDa using A-431 lysate.

Anti-WWP1 antibody produced in rabbit - SAB2102717 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-WWP1: <B>Cat. No.SAB2102717</B>: WWP1 in 293T cell lysate with WWP1 antibody at 1.0 μg/mL.
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