Gene Symbol | FTO |
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Entrez Gene | 79068 |
Alt Symbol | ALKBH9 |
Species | Human |
Gene Type | protein-coding |
Description | fat mass and obesity associated |
Other Description | AlkB homolog 9|alpha-ketoglutarate-dependent dioxygenase FTO|fat mass and obesity-associated protein|protein fto |
Swissprots | Q9C0B1 B2RNS0 A2RUH1 Q7Z785 Q0P676 |
Accessions | EAW82811 Q9C0B1 AB051539 BAB21843 AK096554 BAG53322 AK295196 BAG58196 BC001284 BC003583 AAH03583 BC030798 AAH30798 BC132892 AAI32893 BC137091 AAI37092 BC148442 AAI48443 BC153032 AAI53033 DA324897 U79260 AAB50206 XM_011523313 XP_011521615 XM_011523314 XP_011521616 XM_011523315 XP_011521617 XM_011523316 XP_011521618 NM_001080432 NP_001073901 |
Function | Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. {ECO:0000269|PubMed:18775698, ECO:0000269|PubMed:20376003, ECO:0000269|PubMed:22002720}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:22002720}. Nucleus speckle {ECO:0000269|PubMed:22002720}. |
Tissue Specificity | Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. {ECO:0000269|PubMed:17434869, ECO:0000269|PubMed:17496892}. |
Fatso CRISPR/Cas9 KO Plasmid (h) - sc-403708 from Santa Cruz Biotechnology
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Fatso HDR Plasmid (h) - sc-403708-HDR from Santa Cruz Biotechnology
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Fatso Double Nickase Plasmid (h) - sc-403708-NIC from Santa Cruz Biotechnology
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Fatso Double Nickase Plasmid (h2) - sc-403708-NIC-2 from Santa Cruz Biotechnology
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Fatso CRISPR Activation Plasmid (h) - sc-403708-ACT from Santa Cruz Biotechnology
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Fatso CRISPR Activation Plasmid (h2) - sc-403708-ACT-2 from Santa Cruz Biotechnology
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Fatso Lentiviral Activation Particles (h) - sc-403708-LAC from Santa Cruz Biotechnology
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Fatso Lentiviral Activation Particles (h2) - sc-403708-LAC-2 from Santa Cruz Biotechnology
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Fatso siRNA (h) - sc-75002 from Santa Cruz Biotechnology
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Fatso shRNA Plasmid (h) - sc-75002-SH from Santa Cruz Biotechnology
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Fatso shRNA (h) Lentiviral Particles - sc-75002-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU092071 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN8982 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND8982 from Sigma-Aldrich
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FTO siRNA (Human) - CRJ2288 from Cohesion Biosciences
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FTO - MBS8213136 from MyBioSource
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shRNA set against Human FTO (NM_001080432.2) - SHH297549 from Creative biogene
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FTO shRNA Plasmid (Human) - CHJ2288 from Cohesion Biosciences
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