TAF8

Gene Symbol TAF8
Entrez Gene 129685
Alt Symbol 43, II, TAF, TAFII-43, TAFII43, TBN
Species Human
Gene Type protein-coding
Description TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
Other Description TAF(II)43|TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa|TBP-associated factor 43 kDa|TBP-associated factor 8|TBP-associated factor TAFII43|TBP-associated factor, RNA polymerase II, 43 kD|hTAFII43|protein taube nuss|taube nuss homolog|transcription initiation factor TFIID 43 kDa subunit|transcription initiation factor TFIID subunit 8
Swissprots Q96M52 Q5T0K1 Q7Z7C8 Q8N4R9
Accessions EAX04078 EAX04079 EAX04080 Q7Z7C8 AF086309 AF465841 AAP47094 AK057383 BAB71460 AK303097 BAG64207 AL833401 BC018796 BC033728 BC119678 AAI19679 BC119679 AAI19680 BI828714 XM_005248849 XP_005248906 XM_011514296 XP_011512598 XM_011514297 XP_011512599 NM_138572 NP_612639
Function Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000269|PubMed:14580349}. Cytoplasm {ECO:0000250}. Note=Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell. {ECO:0000250}.
Top Pathways Basal transcription factors

TAF II p43 (B-7) - sc-398062 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TAF II p43 (B-7): sc-398062. Western blot analysis of TAF II p43 expression in NTERA-2 cl.D1 (A), HeLa (B) and 293 (C) whole cell lysates and human heart tissue extract (D).

TAF II p43 (H-271) - sc-368632 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

TAF II p43 (P-13) - sc-109442 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Listeria monocytogenes I/II antibody - ab78729 from Abcam

Prices $370.00
Sizes 2 ml
Host Rabbit
Clonality Polyclonal

Anti-TAF8 antibody - ab77059 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-TAF8 antibody (ab77059) at 5 µg/mlLane 1 : TAF8 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Anti-TAF8 antibody - ab114114 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of Human TAF8 by Western blot of Immunoprecipitate.  TAF8 was immunoprecipitated from 293T whole cell lysate, using ab114114 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). ab114114 was used for the subsequent WB at 1 µg/ml.  Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-TAF8 antibody - ab118730 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-TAF8 antibody (ab118730) at 1 µg/ml + Mouse Heart lysate at 10 µg

Anti-TAF8 Antibody (aa77-89) - LS-C108922 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-TAF8 Antibody (aa151-178, APC) - LS-C241514 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF8 Antibody (aa151-178, Biotin) - LS-C241515 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF8 Antibody (aa151-178, FITC) - LS-C241516 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF8 Antibody (aa151-178, AP) - LS-C241513 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF8 Antibody (aa151-178, PE) - LS-C241517 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF8 Antibody (aa109-158) - LS-C145775 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TAF8 antibody LS-C145775 Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml.

Anti-TAF8 Antibody (aa150-178) - LS-C325561 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF8 Antibody (aa151-178, Azide-free, HRP) - LS-C248302 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF8 Antibody (aa260-310) - LS-C289883 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human TAF8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TAF8 antibody used for IP at 6 ug/mg lysate. TAF8 was also immunoprecipitated by rabbit anti-TAF8 antibody BL11241, which recognizes an upstream epitope. For blotting immunoprecipitated TAF8, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-TAF8 Antibody (aa151-178) - LS-C157131 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
TAF8 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

TAF8 Antibody - NBP2-38189 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TAF8 Antibody [NBP2-38189] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Negative Control.  Lane 3: Overexpression lysate

TAF8 Antibody - NBP2-38188 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TAF8 Antibody [NBP2-38188] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Negative Control.  Lane 3: Overexpression lysate

TAF8 Antibody - NBP1-81986 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: TAF8 Antibody [NBP1-81986] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

TAF8 Antibody - H00129685-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

TAF8 Antibody - NBP1-46131 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

TAF8 Antibody - H00129685-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: TAF8 Antibody [H00129685-D01P] - Analysis of TAF8 expression in transfected 293T cell line by TAF8 polyclonal antibody.Lane 1: TAF8 transfected lysate(18.70 KDa).Lane 2: Non-transfected lysate.

Anti-TBN antibody produced in mouse - SAB1408305 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-TBN: <B>Cat. No. SAB1408305</B>: Antibody concentration: 10 μg/mL using HeLa cell.
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