KLHL22

Gene Symbol KLHL22
Entrez Gene 84861
Alt Symbol KELCHL
Species Human
Gene Type protein-coding
Description kelch-like family member 22
Other Description kelch-like 22|kelch-like protein 22
Swissprots B7Z2G1 A8MTV3 D3DX30 A8K3Q4 Q53GT1 Q96KC6 Q96B68
Accessions EAX02961 EAX02962 EAX02963 EAX02964 Q53GT1 AK024369 AK027266 BAB55007 AK222850 BAD96570 AK290669 BAF83358 AK294682 BAH11847 BC015923 AAH15923 CR456352 CAG30238 CU013062 CAK54493 CU013350 CAK54792 DA381792 XM_011530469 XP_011528771 NM_032775 NP_116164 NR_033825
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. {ECO:0000269|PubMed:19995937, ECO:0000269|PubMed:23455478}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:23455478}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:23455478}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:23455478}. Note=Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase.

KLHL22 (D-17) - sc-86509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
KLHL22 (D-17): sc-86509. Western blot analysis of KLHL22 expression in HeLa whole cell lysate (A) and mouse brain tissue extract (B).

KLHL22 (N-16) - sc-86510 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
KLHL22 (N-16): sc-86510. Western blot analysis of KLHL22 expression in mouse brain tissue extract.

Anti-KLHL22 antibody - ab172898 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-KLHL22 antibody (ab172898) at 1/500 dilutionLane 1 : KLHL22-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-KLHL22 antibody - ab111353 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-KLHL22 antibody (ab111353) at 1 µg/ml + Jurkat cell lysate at 10 µg

Anti-KLHL22 antibody [7B10] - ab130223 from Abcam

Host Mouse
Clonality Monoclonal
Clone 7B10
ab130223, at 1/200 dilution, staining KLHL22 in U251 cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments labeled with Alexa Fluor-555 phalloidin.

Anti-KLHL22 Antibody (aa586-614, FITC) - LS-C261440 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KLHL22 Antibody (aa586-614, Azide-free, HRP) - LS-C261441 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KLHL22 Antibody (aa586-614, PE) - LS-C261443 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KLHL22 Antibody (aa585-614) - LS-C322507 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KLHL22 Antibody (aa175-224) - LS-C135849 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
KLHL22 antibody LS-C135849 Western blot of Jurkat lysate.

Anti-KLHL22 Antibody (clone 7B10) - LS-C169259 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7B10
Immunofluorescence of U251 cells using KLHL22 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Anti-KLHL22 Antibody (aa586-614) - LS-C160007 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
KLH22 Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The KLH22 antibody detected the KLH22 protein (arrow).

Anti-KLHL22 Antibody (aa586-614, APC) - LS-C261436 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KLHL22 Antibody (aa586-614, AP) - LS-C261435 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KLHL22 Antibody (aa586-614, Biotin) - LS-C261439 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

KLHL22 Antibody - NBP1-82238 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: KLHL22 Antibody [NBP1-82238] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)<br/>Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

KLHL22 Antibody - NBP2-17081 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: KLHL22 Antibody [NBP2-17081] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.

KLHL22 Antibody (7B10) - NBP1-51656 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7B10
Western Blot: KLHL22 Antibody (7B10) [NBP1-51656] - Analysis using KLHL22 mouse mAb against mouse brain tissues lysate.

KLHL22 Antibody - NBP2-32606 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: KLHL22 Antibody [NBP2-32606] - Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

KLHL22 Antibody - H00084861-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: KLHL22 Antibody [H00084861-B01] - analysis of KLHL22 expression in transfected 293T cell line by KLHL22 MaxPab polyclonal antibody. Lane 1: KLHL22 transfected lysate (69.74 KDa). Lane 2: Non-transfected lysate.

KLHL22 Polyclonal Antibody - bs-7807R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ANTI-KLH22 (C-TERM) antibody produced in rabbit - SAB1304327 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-KLH22 (C-term): <B>Cat. No. SAB1304327</B>: KLH22 Antibody (C-term) western blot analysis in HeLa cell line lysates (35 μg/lane).

Anti-KLHL22 antibody produced in rabbit - HPA040766 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-KLHL22: <B>Cat. No. HPA040766</B>: Immunohistochemical staining of Human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-KLHL22 antibody produced in rabbit - SAB2107560 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-KLHL22: <B>Cat. No. SAB2107560</B>: Western blot analysis of KLHL22 with KLHL22 (kelch-like 22, Drosophila) antibody, against the N terminal of KLHL22 (50 μg) antibody at 1.0 μg/mL.

Anti-KLHL22 antibody produced in rabbit - SAB2700710 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-KLHL22: <B>Cat. No. SAB2700710</B>: Immunohistochemical analysis of paraffin-embedded Hepatoma, using KLHL22 antibody at 1:500 dilution.
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