Gene Symbol | KLHL22 |
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Entrez Gene | 84861 |
Alt Symbol | KELCHL |
Species | Human |
Gene Type | protein-coding |
Description | kelch-like family member 22 |
Other Description | kelch-like 22|kelch-like protein 22 |
Swissprots | B7Z2G1 A8MTV3 D3DX30 A8K3Q4 Q53GT1 Q96KC6 Q96B68 |
Accessions | EAX02961 EAX02962 EAX02963 EAX02964 Q53GT1 AK024369 AK027266 BAB55007 AK222850 BAD96570 AK290669 BAF83358 AK294682 BAH11847 BC015923 AAH15923 CR456352 CAG30238 CU013062 CAK54493 CU013350 CAK54792 DA381792 XM_011530469 XP_011528771 NM_032775 NP_116164 NR_033825 |
Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. {ECO:0000269|PubMed:19995937, ECO:0000269|PubMed:23455478}. |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:23455478}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:23455478}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:23455478}. Note=Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase. |
KLHL22 CRISPR/Cas9 KO Plasmid (h) - sc-408301 from Santa Cruz Biotechnology
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KLHL22 HDR Plasmid (h) - sc-408301-HDR from Santa Cruz Biotechnology
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KLHL22 Double Nickase Plasmid (h) - sc-408301-NIC from Santa Cruz Biotechnology
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KLHL22 Double Nickase Plasmid (h2) - sc-408301-NIC-2 from Santa Cruz Biotechnology
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KLHL22 CRISPR Activation Plasmid (h) - sc-408301-ACT from Santa Cruz Biotechnology
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KLHL22 CRISPR Activation Plasmid (h2) - sc-408301-ACT-2 from Santa Cruz Biotechnology
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KLHL22 Lentiviral Activation Particles (h) - sc-408301-LAC from Santa Cruz Biotechnology
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KLHL22 Lentiviral Activation Particles (h2) - sc-408301-LAC-2 from Santa Cruz Biotechnology
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KLHL22 siRNA (h) - sc-75396 from Santa Cruz Biotechnology
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KLHL22 shRNA Plasmid (h) - sc-75396-SH from Santa Cruz Biotechnology
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KLHL22 shRNA (h) Lentiviral Particles - sc-75396-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU119491 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN11281 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND11281 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR04042 from Sigma-Aldrich
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KLHL22 siRNA (Human) - CRJ3646 from Cohesion Biosciences
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shRNA set against Human KLHL22 (NM_032775.3) - SHH326171 from Creative biogene
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Human KLHL22 siRNA - orb274147 from Biorbyt
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KLHL22 shRNA Plasmid (Human) - CHJ3646 from Cohesion Biosciences
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KLHL22 miRNA 3'UTR clone - MiUTR3H-15183 from Creative biogene
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