IFIT3

Gene Symbol	IFIT3
Entrez Gene	3437
Alt Symbol	CIG-49, GARG-49, IFI60, IFIT4, IRG2, ISG60, P60, RIG-G
Species	Human
Gene Type	protein-coding
Description	interferon-induced protein with tetratricopeptide repeats 3
Other Description	CIG49\|IFI-60K\|IFIT-3\|IFIT-4\|ISG-60\|interferon-induced 60 kDa protein\|interferon-induced protein with tetratricopeptide repeats 4\|retinoic acid-induced gene G protein
Swissprots	O14879 Q99634 Q9BSK7
Accessions	AAC34669 EAW50143 EAW50144 O14879 AF026939 AAB95160 AF026943 AF083470 AAC63524 AK290427 BAF83116 AK297137 BAG59639 AK312675 BC001383 AAH01383 BC004977 AAH04977 BM790031 BT007284 AAP35948 BU509201 BX101054 DA081546 DA160435 DA673951 DB268497 JF432276 ADZ15493 U52513 AAB40606 NM_001031683 NP_001026853 NM_001289758 NP_001276687 NM_001289759 NP_001276688 NM_001549 NP_001540
Function	IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS- mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin- dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2. {ECO:0000269\|PubMed:17050680, ECO:0000269\|PubMed:20686046, ECO:0000269\|PubMed
Subcellular Location	Cytoplasm. Mitochondrion.
Tissue Specificity	Expression significantly higher in peripheral blood mononuclear cells (PBMCs) and monocytes from systemic lupus erythematosus (SLE) patients than in those from healthy individuals (at protein level). Spleen, lung, leukocytes, lymph nodes, placenta, bone marrow and fetal liver. {ECO:0000269\|PubMed:18706081}.

IFIT3 (B-7) - sc-393512 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

IFIT3 (B-7): sc-393512. Western blot analysis of IFIT3 expression in Caki-1 (A), AML-193 (B), HeLa (C) and Hep G2 (D) whole cell lysates.

IFIT3 (E-10) - sc-393396 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

IFIT3 (E-10): sc-393396. Western blot analysis of IFIT3 expression in HeLa (A), Hep G2 (B) and Caki-1 (C) whole cell lysates.

IFIT3 (C-19) - sc-82950 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

IFIT3 (N-17) - sc-82954 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

IFIT3 (N-17): sc-82954. Western blot analysis of IFIT3 expression in CCRF-CEM (A), HL-60 (B), SK-MEL-24 (C) and K-562 (D) whole cell lysates.

IFIT3 (E-22) - sc-133687 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

IFIT3 (E-22): sc-133687. Western blot analysis of IFIT3 expression in HeLa whole cell lysate.

Anti-IFIT3 antibody - ab76818 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-IFIT3 antibody (ab76818) at 1/500 dilution + Human colon lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

Anti-IFIT3 antibody - ab95989 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IFIT3 antibody (ab95989) at 1/5000 dilution + 293T cell lysate at 30 µg

Anti-IFIT3 antibody [1G1] - ab118045 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1G1

All lanes : Anti-IFIT3 antibody [1G1] (ab118045) at 1/2000 dilutionLane 1 : HEK293T cells, transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells, transfected with pCMV6-ENTRY IFIT3 cDNALysates/proteins at 5 µg per lane.

Anti-IFIT3 antibody - C-terminal - ab191689 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IFIT3 antibody - C-terminal (ab191689) at 1 µg/ml + HepG2 cell lysate at 15 µgdeveloped using the ECL technique

Anti-IFIT3 Antibody - LS-C308385 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-IFIT3 Antibody - LS-C310706 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IFIT3 Antibody - LS-C310703 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IFIT3 Antibody (clone 2C10) - LS-C197493 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2C10

Detection limit for recombinant GST tagged IFIT3 is 0.03 ng/ml as a capture antibody.

Anti-IFIT3 Antibody (aa51-100) - LS-C80794 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

IFIT3 antibody LS-C80794 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-IFIT3 Antibody (C-Terminus) - LS-C336159 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of IFIT3 in human liver tissue with IFIT3 antibody at 5 ug/mL.

Anti-IFIT3 Antibody (aa230-490) - LS-C186265 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded HeLa xenograft using IFIT3 antibody at 1:500 dilution.

IFIT3 Antibody (1G1) - NBP2-02148 from Novus Biologicals

Prices	$379.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1G1

Western Blot: IFIT3 Antibody (1G1) [NBP2-02148] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT3.

IFIT3 Antibody - NBP1-56632 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: IFIT3 Antibody [NBP1-56632] - HepG2 tissue lysate at a concentration of 0.25ug/ml.

IFIT3 Antibody - H00003437-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: IFIT3 Antibody [H00003437-B01P] - Analysis of IFIT3 expression in human colon.

IFIT3 Antibody - NBP2-32500 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: IFIT3 Antibody [NBP2-32500] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp

IFIT3 Antibody - 45370002 from Novus Biologicals

Prices	$299.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

IFIT3 Antibody - H00003437-D01P from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: IFIT3 Antibody [H00003437-D01P] - Analysis of IFIT3 expression in human spleen.

IFIT3 Antibody - NBP1-31722 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: IFIT3 Antibody [NBP1-31722] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:1000.

IFIT3 Antibody - H00003437-D01 from Novus Biologicals

Host	Rabbit
Clonality	Polyclonal

IFIT3 Antibody (2C10) - H00003437-M03 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2C10

Sandwich ELISA: IFIT3 Antibody (2C10) [H00003437-M03] - Detection limit for recombinant GST tagged IFIT3 is 0.03 ng/ml as a capture antibody.

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