TBK1
| Gene Symbol | TBK1 |
|---|---|
| Entrez Gene | 29110 |
| Alt Symbol | NAK, T2K |
| Species | Human |
| Gene Type | protein-coding |
| Description | TANK-binding kinase 1 |
| Other Description | NF-kB-activating kinase|NF-kappa-B-activating kinase|serine/threonine-protein kinase TBK1 |
| Swissprots | Q8IYV3 A8K4S4 Q9NUJ5 Q9UHD2 |
| Accessions | EAW97133 Q9UHD2 AA970899 AF174536 AAF69106 AF191838 AAF05989 AK002192 BAA92129 AK291039 BAF83728 AK303683 BAG64676 AW275367 BC034950 AAH34950 DA788357 XM_005268809 XP_005268866 XM_005268810 XP_005268867 XR_944524 XR_944525 NM_013254 NP_037386 |
| Function | Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferatio |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:15485837, ECO:0000269|PubMed:21813773}. Note=Upon mitogen stimulation or triggering of the immune system, TBK1 is recruited to the exocyst by EXOC2. |
| Tissue Specificity | Ubiquitous with higher expression in testis. Expressed in the ganglion cells, nerve fiber layer and microvasculature of the retina. {ECO:0000269|PubMed:10783893, ECO:0000269|PubMed:21447600}. |
| Top Pathways | Hepatitis C, Toll-like receptor signaling pathway, Measles, Influenza A, Cytosolic DNA-sensing pathway |
TBK1 CRISPR/Cas9 KO Plasmid (h) - sc-401066 from Santa Cruz Biotechnology
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TBK1 HDR Plasmid (h) - sc-401066-HDR from Santa Cruz Biotechnology
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TBK1 Double Nickase Plasmid (h) - sc-401066-NIC from Santa Cruz Biotechnology
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TBK1 Double Nickase Plasmid (h2) - sc-401066-NIC-2 from Santa Cruz Biotechnology
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TBK1 CRISPR Activation Plasmid (h) - sc-401066-ACT from Santa Cruz Biotechnology
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TBK1 CRISPR Activation Plasmid (h2) - sc-401066-ACT-2 from Santa Cruz Biotechnology
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TBK1 Lentiviral Activation Particles (h) - sc-401066-LAC from Santa Cruz Biotechnology
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TBK1 Lentiviral Activation Particles (h2) - sc-401066-LAC-2 from Santa Cruz Biotechnology
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TBK1 siRNA (h) - sc-39058 from Santa Cruz Biotechnology
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TBK1 shRNA Plasmid (h) - sc-39058-SH from Santa Cruz Biotechnology
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TBK1 shRNA (h) Lentiviral Particles - sc-39058-V from Santa Cruz Biotechnology
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TBK1 RNAi - H00029110-R02 from Novus Biologicals
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MISSION® esiRNA - EHU034511 from Sigma-Aldrich
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MISSION® siRNA - SIHK2248 from Sigma-Aldrich
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MISSION® siRNA - SIHK2246 from Sigma-Aldrich
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MISSION® siRNA - SIHK2247 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2384 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2384 from Sigma-Aldrich
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TBK1 siRNA (Human) - CRH9201 from Cohesion Biosciences
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TBK1 - MBS8210471 from MyBioSource
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shRNA set against Human TBK1 (NM_013254.3) - SHH424192 from Creative biogene
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Human TBK1 siRNA - orb269703 from Biorbyt
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TBK1 shRNA Plasmid (Human) - CHH9201 from Cohesion Biosciences
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TBK1 miRNA 3'UTR clone - MiUTR3H-07131 from Creative biogene
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