SMEK1

Gene Symbol SMEK1
Entrez Gene 55671
Alt Symbol FLFL1, KIAA2010, MSTP033, PP4R3A, smk-1, smk1
Species Human
Gene Type protein-coding
Description SMEK homolog 1, suppressor of mek1 (Dictyostelium)
Other Description serine/threonine-protein phosphatase 4 regulatory subunit 3A
Swissprots Q86YI7 Q6IN85 Q9NV01 Q9H7U8 Q9H3F1 Q86U23 Q9NWP1 Q69YK6 Q8IVG1
Accessions EAW81452 EAW81453 EAW81454 EAW81455 EAW81456 EAW81457 Q6IN85 AB095930 BAC23106 AF113213 AAG39284 AI608781 AK000714 BAA91338 AK001885 BAA91960 AK024297 BAB14877 AL832921 CAH10634 BC018617 BC022778 BC028088 AAH28088 BC038932 AAH38932 BC072409 AAH72409 BM147425 BX248247 CAD62575 XM_005267842 XP_005267899 XM_006720194 XP_006720257 XM_011536936 XP_011535238 XR_245703 XR_943491 NM_017936 NM_032560 NM_001284280 NP_001271209 NM_001284281 NP_001271210
Function Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. {ECO:0000269|PubMed:18614045}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:18487071}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:18487071}. Nucleus {ECO:0000269|PubMed:18487071}. Note=In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions.
Top Pathways Glucagon signaling pathway

SMEK1 (C-20) - sc-244184 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SMEK1 (C-20): sc-244184. Western blot analysis of SMEK1 expression in Hep G2 whole cell lysate (A) and human tonsil tissue extract (B).

SMEK1 (E-20) - sc-244182 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SMEK1 (S-20) - sc-244183 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Smek1 antibody - ab70635 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and breast carcinoma (right) tissues labelling Smek1 with ab70635 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection: DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).

Anti-Smek1 antibody - ab199787 from Abcam

Prices $359.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-Smek1 antibody (ab199787) at 1/500 dilution + 293T cell lysate

Anti-Smek1 antibody - ab91618 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab91618, at 1/250 dilution, staining Smek1 in formalin-fixed paraffin-embedded Human stomach carcinoma tissue by Immunohistochemistry using DAB staining.

Anti-Smek1 antibody - ab63968 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Smek1 antibody (ab63968) at 0.3 µg/ml + Human Pancreas lysate at 35 µg

Anti-Smek1 antibody - ab102096 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Smek1 antibody (ab102096) at 1 µg/ml + HeLa cell lysate at 10 µg

Anti-SMEK1 Antibody (C-Terminus) - LS-C91596 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-SMEK1 Antibody (aa600-650) - LS-C287687 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMEK1 Antibody (C-Terminus) - LS-C287688 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PPP4R3 Alpha by Immunohistochemistry and Immunofluorescence. Sample: FFPE sections of human colon carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti-PPP4R3 Alpha used at a dilution of 1:200 (1 ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.

Anti-SMEK1 Antibody (aa645-657) - LS-C55486 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-SMEK1 Antibody (C-Terminus) - LS-C286686 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse PPP4R3 Alpha by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-PPP4R3 Alpha used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Anti-SMEK1 Antibody (aa611-660) - LS-C116734 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMEK1 antibody LS-C116734 Western blot of HeLa lysate.

Smek1 Antibody - NB100-2879 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Smek1 Antibody [NB100-2879] - Detection of Human and Mouse PPP4R3 Alpha on HeLa whole cell lysate using NB100-2879. PPP4R3 alpha was also immunoprecipitated by rabbit anti-PPP4R3 alpha antibody NB100-2878.

Smek1 Antibody - NBP1-84664 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Smek1 Antibody - NB100-68163 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: Smek1 Antibody [NB100-68163] - (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-SMEK1/KIAA2010 antibody produced in goat - SAB2500959 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SMEK1 / KIAA2010: <B>Cat. No. SAB2500959</B>: (0.3 μg/mL) staining of Human Pancreas lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-SMEK1 antibody produced in rabbit - SAB2106158 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SMEK1: <B>Cat. No. SAB2106158</B>: SMEK1 in Hela cell lysates with SMEK1 antibody at 1.0 μg/mL.

Anti-SMEK1 antibody produced in rabbit - HPA002568 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SMEK1: <B>Cat. No. HPA002568</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human duodenal tissue showing nuclear staining of glandular cells.

SMEK1 antibody - middle region (ARP57688_P050) - ARP57688_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SMEK1--KIAA2010-Antibody - AF2007a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF2007a (0.3 µg/ml) staining of Human Pancreas lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat Anti-SMEK1 / KIAA2010 Antibody (OAEB01255) - OAEB01255 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

SMEK1 antibody - 70R-4029 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

SMEK1 antibody - AP16918PU-N from Acris Antibodies

Prices $310.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
HEK293 overexpressing SMEK1 and probed with AP16918PU-N (mock transfection in first lane), tested by Origene.
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