Gene Symbol | MED28 |
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Entrez Gene | 80306 |
Alt Symbol | 1500003D12Rik, EG1, magicin |
Species | Human |
Gene Type | protein-coding |
Description | mediator complex subunit 28 |
Other Description | endothelial-derived gene 1|endothelial-derived protein 1|mediator of RNA polymerase II transcription subunit 28|mediator of RNA polymerase II transcription, subunit 28 homolog|merlin and Grb2-interacting cytoskeletal protein|tumor angiogenesis marker EG-1 |
Swissprots | Q9H204 Q9BZJ5 |
Accessions | EAW92781 EAW92782 Q9H204 AF317678 AF317679 AF317680 AAK11563 AF318059 AAL26906 AF358829 AAK32724 AK313928 BC011936 AAH11936 DQ893391 ABM84317 DQ896713 ABM87712 HY174042 NM_025205 NP_079481 |
Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. {ECO:0000269|PubMed:15467741}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:15467741}. Cytoplasm {ECO:0000269|PubMed:15467741}. Membrane {ECO:0000269|PubMed:15467741}; Peripheral membrane protein {ECO:0000269|PubMed:15467741}. Note=According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated. |
Tissue Specificity | Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver. {ECO:0000269|PubMed:15467741}. |
Med28 CRISPR/Cas9 KO Plasmid (h) - sc-407371 from Santa Cruz Biotechnology
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Med28 HDR Plasmid (h) - sc-407371-HDR from Santa Cruz Biotechnology
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Med28 Double Nickase Plasmid (h) - sc-407371-NIC from Santa Cruz Biotechnology
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Med28 Double Nickase Plasmid (h2) - sc-407371-NIC-2 from Santa Cruz Biotechnology
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Med28 CRISPR Activation Plasmid (h) - sc-407371-ACT from Santa Cruz Biotechnology
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Med28 CRISPR Activation Plasmid (h2) - sc-407371-ACT-2 from Santa Cruz Biotechnology
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Med28 Lentiviral Activation Particles (h) - sc-407371-LAC from Santa Cruz Biotechnology
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Med28 Lentiviral Activation Particles (h2) - sc-407371-LAC-2 from Santa Cruz Biotechnology
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Med28 siRNA (h) - sc-89259 from Santa Cruz Biotechnology
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Med28 shRNA Plasmid (h) - sc-89259-SH from Santa Cruz Biotechnology
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Med28 shRNA (h) Lentiviral Particles - sc-89259-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU045571 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN13177 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND13177 from Sigma-Aldrich
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MED28 siRNA (Human) - CRJ2842 from Cohesion Biosciences
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shRNA set against Human MED28 (NM_025205.3) - SHH340071 from Creative biogene
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shRNA set against Human MED28(NM_025205.3) - SHH183541 from Creative biogene
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Human MED28 siRNA - orb273343 from Biorbyt
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MED28 shRNA Plasmid (Human) - CHJ2842 from Cohesion Biosciences
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MED28 miRNA 3'UTR clone - MiUTR1H-06190 from Creative biogene
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