FEN1
| Gene Symbol | FEN1 |
|---|---|
| Entrez Gene | 2237 |
| Alt Symbol | FEN-1, MF1, RAD2 |
| Species | Human |
| Gene Type | protein-coding |
| Description | flap structure-specific endonuclease 1 |
| Other Description | DNase IV|FEN-1|flap endonuclease 1|maturation factor 1|maturation factor-1 |
| Swissprots | P39748 |
| Accessions | AAB32522 AAC23394 AAM74238 EAW73972 P39748 AK301743 BAG63206 AK312761 BAG35627 BC000323 AAH00323 BM450370 BP244711 BT019524 AAV38331 CN290841 CR536562 CAG38799 L37374 AAA91331 X76771 CAA54166 NM_004111 NP_004102 |
| Function | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {ECO:0000255|HAMAP-Rule:MF_03140, ECO:0000269|PubMed:10744741, ECO:0000269|PubMed:11986308, ECO:0000269|Pub |
| Subcellular Location | Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. |
| Top Pathways | DNA replication, Base excision repair, Non-homologous end-joining |
FEN-1 CRISPR/Cas9 KO Plasmid (h) - sc-403168 from Santa Cruz Biotechnology
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FEN-1 HDR Plasmid (h) - sc-403168-HDR from Santa Cruz Biotechnology
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FEN-1 Double Nickase Plasmid (h) - sc-403168-NIC from Santa Cruz Biotechnology
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FEN-1 Double Nickase Plasmid (h2) - sc-403168-NIC-2 from Santa Cruz Biotechnology
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FEN-1 CRISPR Activation Plasmid (h) - sc-403168-ACT from Santa Cruz Biotechnology
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FEN-1 CRISPR Activation Plasmid (h2) - sc-403168-ACT-2 from Santa Cruz Biotechnology
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FEN-1 Lentiviral Activation Particles (h) - sc-403168-LAC from Santa Cruz Biotechnology
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FEN-1 Lentiviral Activation Particles (h2) - sc-403168-LAC-2 from Santa Cruz Biotechnology
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FEN-1 siRNA (h) - sc-37795 from Santa Cruz Biotechnology
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FEN-1 shRNA Plasmid (h) - sc-37795-SH from Santa Cruz Biotechnology
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FEN-1 shRNA (h) Lentiviral Particles - sc-37795-V from Santa Cruz Biotechnology
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FEN-1 RNAi - H00002237-R01 from Novus Biologicals
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MISSION® esiRNA - EHU110781 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN8620 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND8620 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR05888 from Sigma-Aldrich
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FEN1 siRNA (Human) - CRH1559 from Cohesion Biosciences
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FEN1 - MBS8221334 from MyBioSource
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FEN-1 CRISPR/Cas9 KO Plasmid (h2) - sc-403168-KO-2 from Santa Cruz Biotechnology
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FEN-1 HDR Plasmid (h2) - sc-403168-HDR-2 from Santa Cruz Biotechnology
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Human FEN1 siRNA - orb262061 from Biorbyt
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FEN1 shRNA Plasmid (Human) - CHH1559 from Cohesion Biosciences
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FEN1 miRNA 3'UTR clone - MiUTR3H-03334 from Creative biogene
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