EEF2K

Gene Symbol EEF2K
Entrez Gene 29904
Alt Symbol HSU93850, eEF-2K
Species Human
Gene Type protein-coding
Description eukaryotic elongation factor 2 kinase
Other Description calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase|calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase|calmodulin-dependent protein kinase III|eEF-2 kinase|elongation factor-2 kinase|eukaroytic elongation factor 2 kinase|eukaryotic elongation factor-2 kinase
Swissprots O00418 Q8N588
Accessions EAW50604 O00418 AK022569 AK308757 AW675420 BC032665 AAH32665 CA397138 DA556486 DA735877 DQ890896 ABM81822 DQ894050 ABM84976 U93850 AAB58270 XR_950786 XR_950787 NM_013302 NP_037434
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. {ECO:0000269|PubMed:14709557, ECO:0000269|PubMed:9144159}.
Top Pathways Oxytocin signaling pathway, AMPK signaling pathway

Phospho-eEF2k (Ser366) Antibody - 3691 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody #3692 (lower).

eEF2k Antibody - 3692 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C6, 293, HeLa and SW-13 cells using eEF2k Antibody.

eEF2K (B-4) - sc-393366 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eEF2K (B-4): sc-393366. Western blot analysis of eEF2K expression in non-transfected: sc-117752 (A) and human eEF2K transfected: sc-115200 (B) 293T whole cell lysates.

eEF2K (K-19) - sc-21642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eEF2K (K-19): sc-21642. Western blot analysis of eEF2K expression in untreated (A) and serum starved (B) HeLa whole cell lysates.

p-eEF2K (F-8) - sc-377537 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of eEF2K phosphorylation in untreated (A) and lambda protein phosphatase (sc-200312A) treated (B) MIA PaCa-2 whole cell lysates. Antibodies tested include p-eEF2K (F-8): sc-377537.

eEF2K (C-12) - sc-390710 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of eEF2K phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) MIA PaCa-2 whole cell lysates. Antibodies tested include p-eEF2K (C-4): sc-377562 (A,B) and eEF2K (C-12): sc-390710 (C,D).

p-eEF2K (H-2) - sc-377536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of eEF2K phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) MIA PaCa-2 whole cell lysates. Antibodies tested include p-eEF2K (H-2): sc-377536 (A,B) and eEF2K (C-12): sc-390710 (C,D).

eEF2K (N-14) - sc-21641 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eEF2K (N-14): sc-21641. Western blot analysis of eEF2K expression in HeLa whole cell lysate.

eEF2K (H-300) - sc-292789 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-eEF2K (Ser 359) - sc-21644 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-eEF2K (Ser 359)-R: sc-21644-R. Western blot analysis of eEF2K phosphorylation in HeLa whole cell lysate.

p-eEF2K (Ser 366) - sc-21645 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-eEF2K (Ser 366): sc-21645. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.

p-eEF2K (Ser 396)-R - sc-21646-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
p-eEF2K (Ser 396): sc-21646. Western blot analysis of eEF2K phosphorylation in untreated (A) and serum starved, PMA-treated HeLa (B) whole cell lysates.

p-eEF2K (C-4) - sc-377562 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of eEF2K phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) MIA PaCa-2 whole cell lysates. Antibodies tested include p-eEF2K (C-4): sc-377562 (A,B) and eEF2K (C-12): sc-390710 (C,D).

p-eEF2K (Ser 78) - sc-33051 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-eEF2K (Ser 78): sc-33051. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells and interstitial cells.

Anti-EEF2K (phospho S359) antibody - ab111511 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111511, at 1/50 dilution, staining EEF2K in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-EEF2K (phospho S359) antibody [EPR2252(N)] - ab192257 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2252(N)
All lanes : Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257) at 1/1000 dilutionLane 1 : HeLa cells, treated with Okadaic Acid + Calyculin ALane 2 : HeLa cells, untreatedLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-EEF2K (phospho S366) antibody - ab51227 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilutionLane 1 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins.

Anti-EEF2K antibody - ab85721 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EEF2K with ab85721 at 1/1000 (1µg/ml). Detection: DAB.

Anti-EEF2K antibody - ab96685 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2K antibody (ab96685) at 1/5000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-EEF2K antibody - ab84756 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84756, at 1/100 dilution, staining EEF2K in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.

Anti-EEF2K antibody - ab51213 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2K antibody (ab51213)Lane 1 : Extracts from Hela cells treated with serum (10%, 15mins) with no immunising peptideLane 2 : Extracts from Hela cells treated with serum (10%, 15mins) with immunising peptide

Anti-EEF2K antibody - ab136068 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-EEF2K antibody (ab136068) at 1/500 dilution + K562 cell lysate at 30 µg

Anti-EEF2K antibody [EP724Y] - ab46787 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP724Y
All lanes : Anti-EEF2K antibody [EP724Y] (ab46787) at 1/50000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-EEF2K antibody [EP881Y] - ab45168 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP881Y
Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution + HEK293 cell lysate

Anti-EEF2K Antibody (N-Terminus) - LS-C6489 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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