HECW2

Gene Symbol HECW2
Entrez Gene 57520
Alt Symbol NEDL2
Species Human
Gene Type protein-coding
Description HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Other Description E3 ubiquitin-protein ligase HECW2|HECT, C2 and WW domain-containing protein 2|NEDD4-like E3 ubiquitin-protein ligase 2|NEDD4-related E3 ubiquitin ligase NEDL2
Swissprots Q9NPS9 Q68DF8 Q17RT5 B8ZZB4 Q9P2P5
Accessions EAW70123 Q9P2P5 AB037722 BAA92539 AL390186 CAB99103 BC117194 AAI17195 BC117198 AAI17199 CR749424 CAH18262 XM_006712646 XP_006712709 XM_006712648 XP_006712711 NM_001304840 NP_001291769 NM_020760 NP_065811
Function E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. {ECO:0000269|PubMed:12890487}.
Subcellular Location Cytoplasm {ECO:0000250}.
Tissue Specificity Predominantly expressed in adult brain, lung and heart. {ECO:0000269|PubMed:12890487}.

NEDL2 (I-14) - sc-168731 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NEDL2 (I-14): sc-168731. Western blot analysis of NEDL2 expression in Hep G2 whole cell lysate.

Anti-NEDL2 antibody - ab92711 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab92711 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92711 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NEDL2 antibody - ab129929 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NEDL2 antibody (ab129929) at 1/100 dilution + Mouse brain tissue lysates at 35 µg

Anti-NEDL2 antibody - ab57499 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57499 NEDL2 antibody at 1ug/ml.

Anti-NEDL2 antibody - N-terminal - ab154888 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded Human A549 xenograft tissue, labeling NEDL2 using ab154888 at 1/100 dilution.

Anti-NEDL2 / HECW2 Antibody (aa481-530) - LS-C119928 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from LOVO/HepG2 cells, using HECW2 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-NEDL2 / HECW2 Antibody (aa848-876) - LS-C166830 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Anti-NEDL2 / HECW2 Antibody (aa11-23) - LS-C54772 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-NEDL2 / HECW2 Antibody (aa848-876, AP) - LS-C236160 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDL2 / HECW2 Antibody (clone 1A3) - LS-C139368 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A3

Anti-NEDL2 / HECW2 Antibody (aa848-876, APC) - LS-C236161 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDL2 / HECW2 Antibody (aa848-876, FITC) - LS-C236163 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDL2 / HECW2 Antibody (aa848-876, Biotin) - LS-C236162 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDL2 / HECW2 Antibody (aa848-876, PE) - LS-C236165 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDL2 / HECW2 Antibody (aa848-876, Azide-free, HRP) - LS-C249175 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDL2 / HECW2 Antibody (aa847-876) - LS-C321919 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

NEDL2 Antibody - NBP2-19508 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NEDL2 Antibody [NBP2-19508] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.

NEDL2 Antibody - NBP1-92174 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: NEDL2 Antibody [NBP1-92174] - Staining of human cell line A-431 shows localization to endoplasmic reticulum.

NEDL2 Antibody (5H1) - H00057520-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5H1

NEDL2 Antibody (1A3) - H00057520-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A3

Anti-HECW2 antibody produced in rabbit - SAB4502511 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-HECW2: <B>Cat. No. SAB4502511</B>: Immunoblotting analysis of extracts from LOVO/HepG2 cells, using Anti-HECW2 antibody. The lane on the left was treated with the Anti-HECW2 antibody. The lane on the right (negative control) was treated with both Anti-HECW2 antibody and the synthesized immunogen peptide.

Monoclonal Anti-HECW2 antibody produced in mouse - WH0057520M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5H1
<B>Immunoblotting</B><BR/>Monoclonal Anti-HECW2, <B>Cat. No. WH0057520M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~38.1 kDa using immunogen protein lysate.

Monoclonal Anti-HECW2 antibody produced in mouse - SAB1405215 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A3
<B>Immunoblotting</B><BR/>Monoclonal Anti-HECW2: <B>Cat. No. SAB1405215</B>: Antibody concentration: 1 μg/mL. Specific band of ~38.1 kDa using immunogen protein lysate.

Anti-HECW2 antibody produced in rabbit - HPA034609 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-HECW2: <B>Cat. No. HPA034609</B>: Immunohistochemical staining of human lung shows strong cytoplasmic positivity in subsets of alveolar cells.

HECW2-Antibody-Center - AP16705c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
HECW2 Antibody (Center) (Cat. #AP16705c) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the HECW2 antibody detected the HECW2 protein (arrow).
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