ADAM12

Gene Symbol ADAM12
Entrez Gene 8038
Alt Symbol ADAM12-OT1, CAR10, MCMP, MCMPMltna, MLTN, MLTNA
Species Human
Gene Type protein-coding
Description ADAM metallopeptidase domain 12
Other Description disintegrin and metalloproteinase domain-containing protein 12|meltrin-alpha|metalloprotease-disintegrin 12 transmembrane
Swissprots Q6UWB0 Q5JRP0 O60470 Q5JRP1 Q6P9E3 O43184
Accessions CBH19188 EAW49205 EAW49206 EAW49207 EAW49208 EAW49209 O43184 AF023476 AAC08702 AF023477 AAC08703 AK055628 AK127856 AK291629 BAF84318 AY358878 AAQ89237 BC060804 AAH60804 BX098286 CB960379 DA027173 DA863951 DR004947 HG975387 KF444157 AGX93290 T91975 NM_001288973 NP_001275902 NM_001288974 NP_001275903 NM_001288975 NP_001275904 NM_003474 NP_003465 NM_021641 NP_067673
Function Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). {ECO:0000250}.
Subcellular Location Isoform 3: Secreted {ECO:0000305}.
Tissue Specificity Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.

ADAM12 (C-20) - sc-16527 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ADAM12 (H-210) - sc-25579 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ADAM12 (H-210): sc-25579. Western blot analysis of ADAM12 precursor expression in F9 (A), JAR (B), Sol8 (C) and BT-20 (D) whole cell lysates.

ADAM12 (S-18) - sc-16526 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ADAM12 (S-18): sc-16526. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing membrane, cytoplasmic and nuclear staining of trophoblastic cells.

Anti-ADAM12 antibody - ab28225 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ADAM12 antibody (ab28225)Lane 1 : Human LNCaP prostate cancer cells - whole cell lysateLane 2 : Human LNCaP prostate cancer cells - whole cell lysateLysates/proteins at 25 µg per lane.SecondaryHRP donkey anti-rabbit polyclonal antibodydeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 68,92 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-ADAM12 antibody - ab28747 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)

Anti-ADAM12 antibody - ab115203 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab115203 at 3.75 µg/ml staining ADAM12 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.

Anti-ADAM12 antibody - ab56366 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56366 ADAM12 antibody at 1ug/ml.

Anti-ADAM12 antibody - ab11536 from Abcam

Prices $359.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM12 antibody - Aminoterminal end - ab39155 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ADAM12 antibody - Aminoterminal end (ab39155) at 1/1000 dilution + Heart (Human) Tissue Lysate - adult normal tissue (ab29431) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

Anti-ADAM12 antibody - Cytoplasmic domain - ab39156 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab39156 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39156, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ADAM12 antibody - N-terminal - ab137468 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-ADAM12 antibody - N-terminal (ab137468) at 1/3000 dilution + A431 whole cell lysate at 30 µg

Anti-ADAM12 Antibody (aa29-513) - LS-C124905 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-ADAM12 Antibody - LS-C309323 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM12 Antibody (Catalytic Domain) - LS-C343826 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ADAM12 Antibody (aa216-265) - LS-C110628 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ADAM12 antibody LS-C110628 Western blot of Placenta lysate.

Anti-ADAM12 Antibody (aa225-239) - LS-C204962 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ADAM12 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ADAM12 Antibody (aa2-14) IHC-plus™ - LS-B2756 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-ADAM12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2756 concentration 3.75 ug/ml.

Anti-ADAM12 Antibody (clone 1G3) - LS-C198523 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G3
Western Blot analysis of ADAM12 expression in transfected 293T cell line by ADAM12 monoclonal antibody (M01), clone 1G3.Lane 1: ADAM12 transfected lysate (Predicted MW: 80.4 KDa).Lane 2: Non-transfected lysate.

Anti-ADAM12 Antibody (aa891-903) - LS-C3536 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ADAM12 Antibody (aa29-513, Biotin) - LS-C124904 from LifeSpan Bioscience

Prices $650.00
Sizes 50 µg
Host Sheep
Clonality Polyclonal

Anti-ADAM12 Antibody (Cytoplasmic Domain) - LS-C126600 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM12 Antibody (clone 11n1) - LS-C184170 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 11n1

Anti-ADAM12 Antibody (N-Terminus) - LS-C126602 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM12 Antibody (aa740-752) - LS-C154868 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

ADAM12 Antibody - NB300-889 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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