RGMA

Gene Symbol RGMA
Entrez Gene 56963
Alt Symbol RGM
Species Human
Gene Type protein-coding
Description repulsive guidance molecule family member a
Other Description RGM domain family, member A|repulsive guidance molecule A
Swissprots Q0JV97 Q96B86 F5GZU6 Q9NPM3 Q8NC80 B2RTW1 B7Z5S8 F5GXQ7 G3V518 Q9H0E6
Accessions ACS13733 CAF86163 CAF86186 CAF86838 EAX02166 EAX02167 Q96B86 AK074910 BAC11285 AK074966 BAC11321 AK074980 BAC11330 AK125047 AK125204 BAG54165 AK291365 BAF84054 AK299363 BAH13014 AK309814 AL136826 CAB66760 AL390083 CAB98207 AM392685 CAL37563 BC015886 AAH15886 BC127826 AAI27827 BC140838 AAI40839 BC146682 AAI46683 BC151132 AAI51133 DA183348 DA295545 DA453869 DA512691 DA805034 DC317516 NM_001166283 NP_001159755 NM_001166286 NP_001159758 NM_001166287 NP_001159759 NM_001166288 NP_001159760 NM_001166289 NP_001159761 NM_020211 NP_064596
Function Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. {ECO:0000269|PubMed:19273616, ECO:0000269|PubMed:19458235}.
Subcellular Location Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}.

RGMa (E-10) - sc-393046 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RGMa (E-10): sc-393046. Western blot analysis of RGMa expression in human hippocampus tissue extract.

RGMa (Y-13) - sc-46484 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RGMa (Y-13): sc-46484. Western blot analysis of RGMa expression in HeLa whole cell lysate.

RGMa (H-75) - sc-67052 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RGMa (H-75): sc-67052. Western blot analysis of RGMa expression in mouse brain (A) and rat brain (B) tissue extracts.

RGMa (D-16) - sc-46482 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RGMa (D-16): sc-46482. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and Islets of Langerhans.

RGMa (C-16) - sc-46481 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Repulsive Guidance Molecule A antibody [EP7990(2)] - ab169761 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP7990(2)
Immunofluorescent analysis of HeLa cells labeling Repulsive Guidance Molecule A with ab169761 at 1/50 (red) and DAPI nuclearstaining (blue).

Anti-Repulsive Guidance Molecule A antibody - C-terminal - ab101793 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab101793 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101793 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RGMA Antibody (aa390-418) - LS-C158842 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RGMA Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The RGMA antibody detected the RGMA protein (arrow).

Anti-RGMA Antibody (aa390-418, AP) - LS-C268147 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RGMA Antibody (aa390-418, APC) - LS-C268148 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RGMA Antibody (aa390-418, Biotin) - LS-C268149 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RGMA Antibody (aa20-69) - LS-C186742 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Mouse Heart. Rgma antibody dilution 1.0 ug/ml.

Anti-RGMA Antibody (aa390-418, FITC) - LS-C268150 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RGMA Antibody (aa390-418, PE) - LS-C268151 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RGMA Antibody (aa390-418, Azide-free, HRP) - LS-C268152 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RGMA Antibody (aa389-418) - LS-C324641 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RGMA Antibody - LS-C343696 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

RGM-A Antibody (275407) - MAB2458 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Rat
Clonality Monoclonal
Clone 275407

RGM-A Antibody [Unconjugated] - AF2459 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
RGM‑A was detected in immersion fixed paraffin-embedded sections of human brain (spinal cord) using Goat Anti-Human RGM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2459) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

RGM-A Antibody [Unconjugated] - AF2458 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
RGM‑A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c) using Goat Anti-Mouse RGM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2458) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to retina and developing neurons in cross-section of the developing eye. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

RGM-A Antibody - NBP1-46430 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RGM-A Antibody (6D7) - H00056963-M01-100ug from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D7

RGM-A Antibody [Biotin] - BAF2458 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

RGM-A Antibody [Biotin] - BAF2459 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Monoclonal Anti-RGMA antibody produced in mouse - SAB1412860 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D7
<B>Immunoblotting</B><BR/>Anti-RGMA:<B>Cat. No. SAB1412860</B>: Antibody concentration: 1 μg/mL. Immunofluorescence band of ~36.5 kDa using immunogen protein lysate.
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