ASAH2

Gene Symbol ASAH2
Entrez Gene 56624
Alt Symbol BCDase, HNAC1, LCDase, N-CDase, NCDase
Species Human
Gene Type protein-coding
Description N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Other Description N-acylsphingosine amidohydrolase 2|acylsphingosine deacylase 2|mitochondrial ceramidase|neutral ceramidase|neutral/alkaline ceramidase|non-lysosomal ceramidase
Swissprots Q71ME6 Q5T1D5 Q9NR71 Q5SZP7 Q3KNU1 Q5SNT7 Q5SZP6
Accessions AAL06061 EAW80435 EAW80436 Q9NR71 AF250847 AAF86240 AF449759 AAQ04667 BC107105 AAI07106 BG289059 XM_011539970 XP_011538272 XM_011539971 XP_011538273 XM_011539972 XP_011538274 XM_011539973 XP_011538275 XR_945787 NM_001143974 NP_001137446 NM_019893 NP_063946
Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. {ECO:0000269|PubMed:15946935, ECO:0000269|PubMed:16061940}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:15845354}; Single-pass type II membrane protein {ECO:0000269|PubMed:15845354}. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo.
Tissue Specificity Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. {ECO:0000269|PubMed:10781606, ECO:0000269|PubMed:17334805}.
Top Pathways Sphingolipid signaling pathway, Sphingolipid metabolism

neutral ceramidase (H-300) - sc-68906 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
neutral ceramidase (H-300): sc-68906. Western blot analysis of neutral ceramidase expression in HeLa (A) and KNRK (B) whole cell lysates.

neutral ceramidase (B-9) - sc-374634 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
neutral ceramidase (B-9): sc-374634. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing membrane and cytoplasmic staining of urothelial cells.

neutral ceramidase (N-14) - sc-79642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

neutral ceramidase (C-20) - sc-79641 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

neutral ceramidase (S-20) - sc-79643 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-ASAH2 antibody - ab63804 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ASAH2 antibody (ab63804) at 4 µg/mlLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate

Anti-ASAH2 antibody - ab113369 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-ASAH2 antibody (ab113369) at 1 µg/mlLane 2 : Anti-ASAH2 antibody (ab113369) at 2 µg/mlLane 1 : 293 cell lysateLane 2 : 293 cell lysate

Anti-ASAH2 antibody - C-terminal - ab173358 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-ASAH2 antibody - C-terminal (ab173358) at 1/100 dilution + Mouse bladder tissue lysates at 35 µg

Anti-ASAH2 Antibody (aa734-762, FITC) - LS-C232882 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAH2 Antibody (aa734-762, PE) - LS-C232885 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAH2 Antibody (aa734-762, Azide-free, HRP) - LS-C249468 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAH2 Antibody (aa733-762) - LS-C319619 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAH2 Antibody (aa734-762) - LS-C159128 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ASAH2 Antibody western blot of mouse bladder tissue lysates (35 ug/lane). The ASAH2 antibody detected the ASAH2 protein (arrow).

Anti-ASAH2 Antibody (C-Terminus) IHC-plus™ - LS-B4596 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ASAH2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4596 concentration 5 ug/ml.

Anti-ASAH2 Antibody (aa34-756, Biotin) - LS-C35066 from LifeSpan Bioscience

Prices $650.00
Sizes 50 µg
Host Sheep
Clonality Polyclonal

Anti-ASAH2 Antibody - LS-C146971 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ASAH2 Antibody (aa734-762, APC) - LS-C232879 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAH2 Antibody (aa734-762, Biotin) - LS-C232881 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAH2 Antibody (aa734-762, AP) - LS-C232880 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - AF3557 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal

ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - NBP1-76360 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP1-76360] - ASAH2 Antibody 293 cell lysate with ASAHL antibody at (A) 1 and (B) 2 ug/ml.

ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - NBP1-50671 from Novus Biologicals

Prices $245.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP1-50671] - Analysis of ASAH2 expression with Jurkat call lysate.

Anti-ASAH2 antibody produced in rabbit - SAB2900793 from Sigma-Aldrich

Prices $465.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ASAH2 (Internal), antibody produced in rabbit: <B>Cat. No. SAB2900793</B>: Anti-ASAH2 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 μg/mL.

Anti-ASAH2 antibody produced in rabbit - PRS4743 from Sigma-Aldrich

Prices $347.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ASAH2: <B>Cat. No. PRS4743</B> Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL.

ANTI-ASAH2(C-TERMINAL) antibody produced in rabbit - SAB1305101 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ASAH2 (C-term): <B>Cat. No. SAB1305101</B>: ASAH2 Antibody (C-term) western blot analysis in Mouse bladder tissue lysates (35 μg/lane). This demonstrates the ASAH2 detected the ASAH2 protein (arrow).
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