| Gene Symbol | ASAH2 |
|---|---|
| Entrez Gene | 56624 |
| Alt Symbol | BCDase, HNAC1, LCDase, N-CDase, NCDase |
| Species | Human |
| Gene Type | protein-coding |
| Description | N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 |
| Other Description | N-acylsphingosine amidohydrolase 2|acylsphingosine deacylase 2|mitochondrial ceramidase|neutral ceramidase|neutral/alkaline ceramidase|non-lysosomal ceramidase |
| Swissprots | Q71ME6 Q5T1D5 Q9NR71 Q5SZP7 Q3KNU1 Q5SNT7 Q5SZP6 |
| Accessions | AAL06061 EAW80435 EAW80436 Q9NR71 AF250847 AAF86240 AF449759 AAQ04667 BC107105 AAI07106 BG289059 XM_011539970 XP_011538272 XM_011539971 XP_011538273 XM_011539972 XP_011538274 XM_011539973 XP_011538275 XR_945787 NM_001143974 NP_001137446 NM_019893 NP_063946 |
| Function | Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. {ECO:0000269|PubMed:15946935, ECO:0000269|PubMed:16061940}. |
| Subcellular Location | Cell membrane {ECO:0000269|PubMed:15845354}; Single-pass type II membrane protein {ECO:0000269|PubMed:15845354}. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo. |
| Tissue Specificity | Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. {ECO:0000269|PubMed:10781606, ECO:0000269|PubMed:17334805}. |
| Top Pathways | Sphingolipid signaling pathway, Sphingolipid metabolism |
neutral ceramidase CRISPR/Cas9 KO Plasmid (h) - sc-404967 from Santa Cruz Biotechnology
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neutral ceramidase HDR Plasmid (h) - sc-404967-HDR from Santa Cruz Biotechnology
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neutral ceramidase Double Nickase Plasmid (h) - sc-404967-NIC from Santa Cruz Biotechnology
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neutral ceramidase Double Nickase Plasmid (h2) - sc-404967-NIC-2 from Santa Cruz Biotechnology
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neutral ceramidase CRISPR Activation Plasmid (h) - sc-404967-ACT from Santa Cruz Biotechnology
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neutral ceramidase CRISPR Activation Plasmid (h2) - sc-404967-ACT-2 from Santa Cruz Biotechnology
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neutral ceramidase Lentiviral Activation Particles (h) - sc-404967-LAC from Santa Cruz Biotechnology
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neutral ceramidase Lentiviral Activation Particles (h2) - sc-404967-LAC-2 from Santa Cruz Biotechnology
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neutral ceramidase siRNA (h) - sc-75908 from Santa Cruz Biotechnology
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neutral ceramidase shRNA Plasmid (h) - sc-75908-SH from Santa Cruz Biotechnology
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neutral ceramidase shRNA (h) Lentiviral Particles - sc-75908-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU005221 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3559 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3559 from Sigma-Aldrich
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ASAH2 siRNA (Human) - CRJ1154 from Cohesion Biosciences
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ASAH2 - MBS8233341 from MyBioSource
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Human ASAH2 siRNA - orb271655 from Biorbyt
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ASAH2 shRNA Plasmid (Human) - CHJ1154 from Cohesion Biosciences
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ASAH2 miRNA 3'UTR clone - MiUTR3H-11705 from Creative biogene
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ASAH2 miRNA 3'UTR clone - MiUTR3H-11706 from Creative biogene
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