| Gene Symbol | NEIL3 |
|---|---|
| Entrez Gene | 55247 |
| Alt Symbol | FGP2, FPG2, NEI3, ZGRF3, hFPG2, hNEI3 |
| Species | Human |
| Gene Type | protein-coding |
| Description | nei endonuclease VIII-like 3 (E. coli) |
| Other Description | DNA glycosylase FPG2|DNA glycosylase hFPG2|DNA glycosylase/AP lyase Neil3|endonuclease 8-like 3|endonuclease VIII-like 3|nei-like protein 3|zinc finger, GRF-type containing 3 |
| Swissprots | Q9NV95 Q8NG51 Q2PPJ3 Q8TAT5 |
| Accessions | ABC40719 CDH92855 EAX04716 Q8TAT5 AB079071 BAC06479 AI419161 AK001720 BAA91860 AK307692 BC025954 AAH25954 BE865481 BP380910 CN426741 NM_018248 NP_060718 |
| Function | DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5- OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair. {ECO:0000269|PubMed:12433996, ECO:0000269|PubMed:19170771, ECO:0000269|PubMed:22569481, ECO:0000269|PubMed:23755964}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:12433996}. |
| Tissue Specificity | Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts. {ECO:0000269|PubMed:12433996, ECO:0000269|PubMed:16428305, ECO:0000269|PubMed:19426544, ECO:0000269|PubMed:22365498}. |
| Top Pathways | Base excision repair |
NEIL3 CRISPR/Cas9 KO Plasmid (h) - sc-406572 from Santa Cruz Biotechnology
|
||||||||
NEIL3 HDR Plasmid (h) - sc-406572-HDR from Santa Cruz Biotechnology
|
||||||||
NEIL3 Double Nickase Plasmid (h) - sc-406572-NIC from Santa Cruz Biotechnology
|
||||||||
NEIL3 Double Nickase Plasmid (h2) - sc-406572-NIC-2 from Santa Cruz Biotechnology
|
||||||||
NEIL3 CRISPR Activation Plasmid (h) - sc-406572-ACT from Santa Cruz Biotechnology
|
||||||||
NEIL3 CRISPR Activation Plasmid (h2) - sc-406572-ACT-2 from Santa Cruz Biotechnology
|
||||||||
NEIL3 Lentiviral Activation Particles (h) - sc-406572-LAC from Santa Cruz Biotechnology
|
||||||||
NEIL3 Lentiviral Activation Particles (h2) - sc-406572-LAC-2 from Santa Cruz Biotechnology
|
||||||||
NEIL3 siRNA (h) - sc-61170 from Santa Cruz Biotechnology
|
||||||||
NEIL3 shRNA Plasmid (h) - sc-61170-SH from Santa Cruz Biotechnology
|
||||||||
NEIL3 shRNA (h) Lentiviral Particles - sc-61170-V from Santa Cruz Biotechnology
|
||||||||
NEI3 RNAi - H00055247-R01 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU028691 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN15197 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15197 from Sigma-Aldrich
|
||||||||
MISSION® 3′UTR Lenti GoClone™ - HUTR04753 from Sigma-Aldrich
|
||||||||
NEIL3 siRNA (Human) - CRJ0592 from Cohesion Biosciences
|
||||||||
NEIL3 CRISPR/Cas9 KO Plasmid (h2) - sc-406572-KO-2 from Santa Cruz Biotechnology
|
||||||||
NEIL3 HDR Plasmid (h2) - sc-406572-HDR-2 from Santa Cruz Biotechnology
|
||||||||
shRNA set against Human NEIL3 (NM_018248.2) - SHH351184 from Creative biogene
|
||||||||
NEIL3 shRNA Plasmid (Human) - CHJ0592 from Cohesion Biosciences
|
||||||||
NEIL3 miRNA 3'UTR clone - MiUTR1H-06718 from Creative biogene
|