SRSF1

Gene Symbol SRSF1
Entrez Gene 6426
Alt Symbol ASF, SF2, SF2p33, SFRS1, SRp30a
Species Human
Gene Type protein-coding
Description serine/arginine-rich splicing factor 1
Other Description SR splicing factor 1|alternative-splicing factor 1|pre-mRNA-splicing factor SF2, P33 subunit|splicing factor 2|splicing factor, arginine/serine-rich, 30-KD, A
Swissprots B2R6Z7 D3DTZ3 Q13809 Q07955
Accessions EAW94485 EAW94486 EAW94487 EAW94488 EAW94489 EAW94490 EAW94491 EAW94492 Q07955 AB062124 BAB93456 AB209558 BAD92795 AK054610 AK095112 AK126318 BAC86523 AK225711 AK289933 BAF82622 AK298527 BAH12806 AK312781 BAG35644 BC010264 AAH10264 BC033785 AAH33785 CB044653 DA939911 HG509274 M69040 AAA03476 M72709 AAA35564 XM_006722012 XP_006722075 XR_429911 XR_429912 NM_001078166 NP_001071634 NM_006924 NP_008855 NR_034041
Function Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. {ECO:0000269|PubMed:8139654}.
Subcellular Location Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm.
Top Pathways Spliceosome, Herpes simplex infection

SF2/ASF Antibody - 8241 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using SF2/ASF Antibody.

SF2/ASF (P-15) - sc-10254 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SF2/ASF (P-15): sc-10254. Western blot analysis of SF2/ASF expression in HeLa (A) and K-562 (B) nuclear extracts.

SF2/ASF (C-19) - sc-10255 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SF2/ASF (C-19): sc-10255. Western blot analysis of SF2/ASF expression in Raji whole cell lysate.

SF2/ASF (96) - sc-33652 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SF2/ASF (96): sc-33652. Western blot analysis of SF2/ASF expression in HeLa (A), K-562 (B) nuclear extracts, Raji whole cell lysate (C) and Jurkat nuclear extract (D).

SF2/ASF (3G268) - sc-73026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SF2/ASF (3G268): sc-73026. Western blot analysis of SF2/ASF expression in non-transfected 293T: sc-117752 (A), human SF2/ASF transfected 293T: sc-113535 (B) and Raji (C) whole cell lysates.

SF2/ASF/SRp30 (H-110) - sc-28724 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SF2/ASF/SRp30 (H-110): sc-28724. Western blot analysis of SF2/ASF/SRp30 expression in 293T whole cell lysate.

SR (1H4) - sc-13509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SR (1H4): sc-13509. Western blot analysis of SR expression in BJAB (A) and DT40 (B) whole cell lysates.

Anti-SF2 antibody - ab38017 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SF2 antibody (ab38017) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-SF2 antibody - ab167515 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-SF2 antibody (ab167515) at 1 µg/mlLane 1 : SF2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse IgG HRP at 1/2500 dilution

Anti-SF2 antibody [EPR8239] - ab129108 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8239
All lanes : Anti-SF2 antibody [EPR8239] (ab129108) at 1/10000 dilution (purified)Lane 1 : HepG2 cell lysateLane 2 : Raji cell lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-SF2 antibody [EPR8239] (Alexa Fluor® 488) - ab197877 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8239
Overlay histogram showing HepG2 cells stained with ab197877 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197877, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SF2 antibody [EPR8239] (Alexa Fluor® 647) - ab197878 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8239
Overlay histogram showing HepG2 cells stained with ab197878 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197878, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

Anti-SF2 antibody [EPR8239] (HRP) - ab198552 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8239
All lanes : Anti-SF2 antibody [EPR8239] (HRP) (ab198552) at 1/5000 dilutionLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-SF2 antibody [EPR8240] - ab133689 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8240
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SF2 with ab133689 at 1/50 dilution.

Anti-SF2 antibody - C-terminal - ab135617 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SF2 antibody - C-terminal (ab135617) at 1/50 dilution + HL60 cell line lysate at 35 µgdeveloped using the ECL technique

Anti-SFRS1 / SF2 Antibody (aa198-248) - LS-C288810 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SFRS1 / SF2 Antibody (N-Terminus) - LS-C328735 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SFRS1 / SF2 Antibody - LS-C193020 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot.

Anti-SFRS1 / SF2 Antibody (Internal, clone EPR8240) - LS-C154636 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8240
IHC of paraffin-embedded human colon tissue using anti-SF2 antibody.

Anti-SFRS1 / SF2 Antibody (N-Terminus) IHC-plus™ - LS-B2340 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Anti-SF2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2340 concentration 5 ug/ml.

Anti-SFRS1 / SF2 Antibody (aa17-47) - LS-C169020 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SFRS1 Antibody IHC of formalin-fixed and paraffin-embedded testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-SFRS1 / SF2 Antibody (aa165-194) - LS-C169019 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-SFRS1 / SF2 Antibody (N-Terminus) IHC-plus™ - LS-B3263 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Anti-SF2 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3263 concentration 10 ug/ml.

Anti-SFRS1 / SF2 Antibody - LS-C331609 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human stomach cancer using SFRS1 antibody at dilution of 1:200 (400x lens).

Anti-SFRS1 / SF2 Antibody (Internal, clone EPR8239) IHC-plus™ - LS-B11069 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8239
Anti-SFRS1 / SF2 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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