SRSF1
| Gene Symbol | SRSF1 |
|---|---|
| Entrez Gene | 6426 |
| Alt Symbol | ASF, SF2, SF2p33, SFRS1, SRp30a |
| Species | Human |
| Gene Type | protein-coding |
| Description | serine/arginine-rich splicing factor 1 |
| Other Description | SR splicing factor 1|alternative-splicing factor 1|pre-mRNA-splicing factor SF2, P33 subunit|splicing factor 2|splicing factor, arginine/serine-rich, 30-KD, A |
| Swissprots | B2R6Z7 D3DTZ3 Q13809 Q07955 |
| Accessions | EAW94485 EAW94486 EAW94487 EAW94488 EAW94489 EAW94490 EAW94491 EAW94492 Q07955 AB062124 BAB93456 AB209558 BAD92795 AK054610 AK095112 AK126318 BAC86523 AK225711 AK289933 BAF82622 AK298527 BAH12806 AK312781 BAG35644 BC010264 AAH10264 BC033785 AAH33785 CB044653 DA939911 HG509274 M69040 AAA03476 M72709 AAA35564 XM_006722012 XP_006722075 XR_429911 XR_429912 NM_001078166 NP_001071634 NM_006924 NP_008855 NR_034041 |
| Function | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. {ECO:0000269|PubMed:8139654}. |
| Subcellular Location | Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm. |
| Top Pathways | Spliceosome, Herpes simplex infection |
SF2/ASF CRISPR/Cas9 KO Plasmid (h) - sc-400737 from Santa Cruz Biotechnology
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SF2/ASF HDR Plasmid (h) - sc-400737-HDR from Santa Cruz Biotechnology
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SF2/ASF Double Nickase Plasmid (h) - sc-400737-NIC from Santa Cruz Biotechnology
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SF2/ASF Double Nickase Plasmid (h2) - sc-400737-NIC-2 from Santa Cruz Biotechnology
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SF2/ASF CRISPR Activation Plasmid (h) - sc-400737-ACT from Santa Cruz Biotechnology
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SF2/ASF CRISPR Activation Plasmid (h2) - sc-400737-ACT-2 from Santa Cruz Biotechnology
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SF2/ASF Lentiviral Activation Particles (h) - sc-400737-LAC from Santa Cruz Biotechnology
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SF2/ASF Lentiviral Activation Particles (h2) - sc-400737-LAC-2 from Santa Cruz Biotechnology
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SF2/ASF siRNA (h) - sc-38319 from Santa Cruz Biotechnology
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SF2/ASF shRNA Plasmid (h) - sc-38319-SH from Santa Cruz Biotechnology
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SF2/ASF shRNA (h) Lentiviral Particles - sc-38319-V from Santa Cruz Biotechnology
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SF2 RNAi - H00006426-R01 from Novus Biologicals
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MISSION® esiRNA - EHU132201 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20597 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20597 from Sigma-Aldrich
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SRSF1 siRNA (Human) - CRH4341 from Cohesion Biosciences
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SRSF1 - MBS8226604 from MyBioSource
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shRNA set against Human SRSF1 (NM_006924.4) - SHH418148 from Creative biogene
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shRNA set against Human SFRS1(NM_001078166.1) - SHR219792 from Creative biogene
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shRNA set against Human SFRS1(NM_006924.4) - SHR219800 from Creative biogene
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shRNA set against Human SFRS1(NM_001078166.1) - SHR219918 from Creative biogene
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shRNA set against Human SFRS1(NM_006924.4) - SHR219928 from Creative biogene
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SRSF1 shRNA Plasmid (Human) - CHH4341 from Cohesion Biosciences
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SRSF1 miRNA 3'UTR clone - MiUTR1H-09329 from Creative biogene
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SRSF1 miRNA 3'UTR clone - MiUTR1H-09330 from Creative biogene
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