DGKE

Gene Symbol	DGKE
Entrez Gene	8526
Alt Symbol	DAGK5, DAGK6, DGK, NPHS7
Species	Human
Gene Type	protein-coding
Description	diacylglycerol kinase, epsilon 64kDa
Other Description	DAG kinase epsilon\|DGK-epsilon\|diacylglycerol kinase epsilon\|diglyceride kinase epsilon
Swissprots	P52429 Q8TBM4 Q9UKQ3
Accessions	AAD45666 EAW94526 P52429 AB209365 BAD92602 AK057591 AK129803 AK293039 BAF85728 AK310668 AL555194 BC022297 AAH22297 BC130629 AAI30630 BC130631 AAI30632 BM682264 DB221284 HQ258315 ADR83069 U49379 AAC50497 XM_011525394 XP_011523696 XM_011525395 XP_011523697 XM_011525396 XP_011523698 XM_011525397 XP_011523699 XM_011525398 XP_011523700 XR_934581 NM_003647 NP_003638
Function	Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Subcellular Location	Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Membrane {ECO:0000269\|PubMed:23542698}. Cytoplasm {ECO:0000269\|PubMed:23542698}.
Tissue Specificity	Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level). {ECO:0000269\|PubMed:23542698}.
Top Pathways	Choline metabolism in cancer, Glycerolipid metabolism, Glycerophospholipid metabolism, Phosphatidylinositol signaling system

DGK-ε (E-19) - sc-18303 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DGK-ε (E-19): sc-18303. Western blot analysis of DGK-ε expression in mouse testis (A) and rat testis (B) tissue extracts.

DGK-ε (N-14) - sc-18302 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DGK-ε (N-14): sc-18302. Western blot analysis of DGK-ε expression in mouse testis tissue extract.

DGK-ε (AA9) - sc-100372 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

DGK-ε (AA9): sc-100372. Western blot analysis of DGK-ε expression in HeLa whole cell lysate.

Anti-DGKE antibody - ab69760 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-DGKE antibody (ab69760) at 1/500 dilutionLane 1 : Extracts from K562 cellsLane 2 : Extracts from K562 cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-DGKE antibody - ab56371 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-DGKE antibody - ab84331 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DGKE antibody (ab84331) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µgSecondaryHRP conjugated anti-Rabbit Ig at 1/50000 dilution

Anti-DGKE antibody [EPR7847] - ab128949 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7847

All lanes : Anti-DGKE antibody [EPR7847] (ab128949) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Hela cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-DGKE / DGK Epsilon Antibody (aa314-435) - LS-C182833 from LifeSpan Bioscience

Prices	$650.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Anti-DGKE / DGK Epsilon Antibody (aa314-435, clone 12K78) - LS-C182834 from LifeSpan Bioscience

Prices	$485.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	12K78

Anti-DGKE / DGK Epsilon Antibody (N-Terminus) - LS-C102174 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

DGKE antibody LS-C102174 Western blot of Fetal Brain lysate.

Anti-DGKE / DGK Epsilon Antibody (aa243-502, Biotin) - LS-C301085 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKE / DGK Epsilon Antibody (aa243-502, FITC) - LS-C304000 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DGKE / DGK Epsilon Antibody (Internal, clone EPR7847) - LS-C152409 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7847

Western blot analysis on (A) 293T, (B) HeLa, and (C) K562 cell lysates using anti-DGKE antibody.

Anti-DGKE / DGK Epsilon Antibody (aa161-210) - LS-C118928 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from K562 cells, using DGKE Antibody. The lane on the right is treated with the synthesized peptide.

Anti-DGKE / DGK Epsilon Antibody (Ser194) - LS-C176912 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of DGK- (S194) pAb in extracts from K562 cells.

Anti-DGKE / DGK Epsilon Antibody (aa141-241) - LS-C307229 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-DGKE / DGK Epsilon Antibody (aa243-502) - LS-C293680 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

DGK-epsilon Antibody (670914) - MAB5125 from Novus Biologicals

Prices	$99.00, $249.00
Sizes	25 µg, 100 µg
Host	Mouse
Clonality	Monoclonal
Clone	670914

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, L1.2 mouse pro-B cell line, L6 rat myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DGK‑ epsilon Monoclonal Antibody (Catalog # MAB5125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK‑ epsilon at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DGK-epsilon Antibody - AF7069 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Western blot shows lysates of Caki‑2 human clear cell carcinoma epithelial cell line, K562 human chronic myelogenous leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DGK‑ epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DGK‑ epsilon at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DGK-epsilon Antibody (7E1.) - H00008526-M03 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	7E1.

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in Raw 264.7 (Cat # L024V1).

DGK-epsilon Antibody - NBP1-85315 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: DGK-epsilon Antibody [NBP1-85315] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm.

DGK-epsilon Antibody - NBP2-16157 from Novus Biologicals

Host	Rabbit
Clonality	Polyclonal

Western Blot: DGKE Antibody [NBP2-16157] - Sample (30 ug of whole cell lysate) A: HUVEC 7. 5% SDS PAGE gel, diluted at 1:1000.

DGK-epsilon Antibody - NBP2-16156 from Novus Biologicals

Host	Rabbit
Clonality	Polyclonal

Western Blot: DGKE Antibody [NBP2-16156] - Sample (30 ug of whole cell lysate) A: HUVEC 7. 5% SDS PAGE gel, diluted at 1:1000.

DGK-epsilon Antibody - NBP1-59067 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: DGK-epsilon Antibody [NBP1-59067] - DGKE antibody - N-terminal region validated by WB using lymph at 1:1000.

DGKE Polyclonal Antibody - bs-12999R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Mouse testis lysates probed with DGKE Polyclonal Antibody, unconjugated (bs-12999R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

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