PADI4

Gene Symbol PADI4
Entrez Gene 23569
Alt Symbol PAD, PAD4, PADI5, PDI4, PDI5
Species Human
Gene Type protein-coding
Description peptidyl arginine deiminase, type IV
Other Description HL-60 PAD|PADI-H protein|peptidyl arginine deiminase, type V|protein-arginine deiminase type IV|protein-arginine deiminase type-4
Swissprots B2RBW0 Q70SX4 Q5VTZ8 Q9UM07 A8K392
Accessions CAE47743 CAG30581 CAG30582 CAG30583 CAG30584 CAG30585 CAG30586 CAG30587 CAI84651 EAW94835 EAW94836 Q9UM07 AB017919 BAA84542 AF229067 AAF82265 AK290507 BAF83196 AK309637 AK314839 BAG37357 BC025718 AAH25718 JF432820 ADZ16037 XM_011541150 XP_011539452 XM_011541151 XP_011539453 XM_011541152 XP_011539454 XM_011541153 XP_011539455 XM_011541154 XP_011539456 XM_011541155 XP_011539457 XM_011541156 XP_011539458 XM_011541157 XP_011539459 XM_011548837 XP_011547139 XM_011548838 XP_011547140 XM_011548839 XP_011547141 XM_011548840 XP_011547142 XM_011548841 XP_011547143 XM_011548842 XP_011547144 XM_011548843 XP_011547145 XM_011548844 XP_011547146 NM_012387 NP_036519
Function Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg- 54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCO
Subcellular Location Cytoplasm. Nucleus. Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils and neutrophils.
Tissue Specificity Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. {ECO:0000269|PubMed:11435484}.

PADI4 (A-11) - sc-365369 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PADI4 (A-11): sc-365369. Western blot analysis of PADI4 expression in HeLa whole cell lysate.

PADI4 (C-3) - sc-166645 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PADI4 (C-3): sc-166645. Western blot analysis of PADI4 expression in non-transfected: sc-117752 (A) and human PADI4 transfected: sc-114204 (B) 293T whole cell lysates.

PADI4 (H-70) - sc-98991 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PADI4 (H-70): sc-98991. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of hematopoietic cells and endothelial cells.

PADI4 (K-18) - sc-47287 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PADI4 (K-18): sc-47287. Western blot analysis of PADI4 expression in mouse PBL whole cell lysate.

Anti-PADI antibody [3E1] - ab140303 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E1
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4, labelling with ab140303 at 1/100 dilution.

Anti-PADI4 / PAD4 antibody - ab50247 from Abcam

Prices $379.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab50247 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50247, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PADI4 / PAD4 antibody - ab96758 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PADI4 / PAD4 antibody (ab96758) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-PADI4 / PAD4 antibody - ab50332 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PADI4 / PAD4 antibody (ab50332) at 1/1000 dilutionLane 1 : 293-T cellsLane 2 : 293-T cells transfected with PADI4 cDNALane 3 : HL60 cellsLane 4 : HL60 cells induced with 1.25% DMSO for three daysSecondaryGoat Anti-Rabbit IgG

Anti-PADI4 / PAD4 antibody - ab59965 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab59965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59965 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PADI4 / PAD4 antibody - ab26071 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-PADI4 / PAD4 antibody (ab26071) at 0.3 µg/ml + human spleen lysate (35µg protein in RIPA buffer). developed using the ECL technique

Anti-PADI4 / PAD4 antibody - ab57167 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57167 PADI4 / PAD4 antibody at 1ug/ml.

Anti-PADI4 / PAD4 antibody - ab24007 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PADI4 / PAD4 antibody [4H5] - ab128086 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4H5
All lanes : Anti-PADI4 / PAD4 antibody [4H5] (ab128086) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY PADI4 / PAD4 cDNA Lysates/proteins at 5 µg per lane.

Anti-PADI4 / PAD4 Antibody (N-Terminus) - LS-C46953 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PADI4 / PAD4 Antibody (C-Terminus) - LS-C144651 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PADI4 / PAD4 Antibody - LS-C150730 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PADI4 / PAD4 Antibody (C-Terminus) - LS-C126106 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PADI4 / PAD4 Antibody (C-Terminus) - LS-C153535 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PADI4 / PAD4 Antibody (aa2-14) - LS-C55226 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.3 ug/ml) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PADI4 / PAD4 Antibody (aa398-447) - LS-C81955 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PADI4 / PAD4 Antibody - LS-C341375 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PADI4 / PAD4 Antibody (aa512-544) - LS-C98530 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-PAD4 antibody in 293 cell line lysate. PAD4(arrow) was detected using the purified antibody.

Anti-PADI4 / PAD4 Antibody (aa492-512) - LS-C313169 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB of PADI4 / PAD4 antibody. Lane 1: PANC Cell Lysate. Lane 2: 293T Cell Lysate..

Anti-PADI4 / PAD4 Antibody (clone 3E1) - LS-C173982 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E1
IHC of paraffin-embedded Human pancreas tissue using anti-PADI4 mouse monoclonal antibody.

Anti-PADI4 / PAD4 Antibody (clone 5B7) - LS-C174036 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5B7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.
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