PADI4
| Gene Symbol | PADI4 |
|---|---|
| Entrez Gene | 23569 |
| Alt Symbol | PAD, PAD4, PADI5, PDI4, PDI5 |
| Species | Human |
| Gene Type | protein-coding |
| Description | peptidyl arginine deiminase, type IV |
| Other Description | HL-60 PAD|PADI-H protein|peptidyl arginine deiminase, type V|protein-arginine deiminase type IV|protein-arginine deiminase type-4 |
| Swissprots | B2RBW0 Q70SX4 Q5VTZ8 Q9UM07 A8K392 |
| Accessions | CAE47743 CAG30581 CAG30582 CAG30583 CAG30584 CAG30585 CAG30586 CAG30587 CAI84651 EAW94835 EAW94836 Q9UM07 AB017919 BAA84542 AF229067 AAF82265 AK290507 BAF83196 AK309637 AK314839 BAG37357 BC025718 AAH25718 JF432820 ADZ16037 XM_011541150 XP_011539452 XM_011541151 XP_011539453 XM_011541152 XP_011539454 XM_011541153 XP_011539455 XM_011541154 XP_011539456 XM_011541155 XP_011539457 XM_011541156 XP_011539458 XM_011541157 XP_011539459 XM_011548837 XP_011547139 XM_011548838 XP_011547140 XM_011548839 XP_011547141 XM_011548840 XP_011547142 XM_011548841 XP_011547143 XM_011548842 XP_011547144 XM_011548843 XP_011547145 XM_011548844 XP_011547146 NM_012387 NP_036519 |
| Function | Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg- 54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCO |
| Subcellular Location | Cytoplasm. Nucleus. Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils and neutrophils. |
| Tissue Specificity | Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. {ECO:0000269|PubMed:11435484}. |
PADI4 CRISPR/Cas9 KO Plasmid (h) - sc-402657 from Santa Cruz Biotechnology
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PADI4 HDR Plasmid (h) - sc-402657-HDR from Santa Cruz Biotechnology
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PADI4 Double Nickase Plasmid (h) - sc-402657-NIC from Santa Cruz Biotechnology
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PADI4 Double Nickase Plasmid (h2) - sc-402657-NIC-2 from Santa Cruz Biotechnology
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PADI4 CRISPR Activation Plasmid (h) - sc-402657-ACT from Santa Cruz Biotechnology
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PADI4 CRISPR Activation Plasmid (h2) - sc-402657-ACT-2 from Santa Cruz Biotechnology
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PADI4 Lentiviral Activation Particles (h) - sc-402657-LAC from Santa Cruz Biotechnology
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PADI4 Lentiviral Activation Particles (h2) - sc-402657-LAC-2 from Santa Cruz Biotechnology
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PADI4 siRNA (h) - sc-61283 from Santa Cruz Biotechnology
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PADI4 shRNA Plasmid (h) - sc-61283-SH from Santa Cruz Biotechnology
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PADI4 shRNA (h) Lentiviral Particles - sc-61283-V from Santa Cruz Biotechnology
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PADI4 RNAi - H00023569-R01 from Novus Biologicals
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MISSION® esiRNA - EHU082591 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN16267 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16267 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR01392 from Sigma-Aldrich
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PADI4 siRNA (Human) - CRH8369 from Cohesion Biosciences
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shRNA set against Human PADI4 (NM_012387.2) - SHH369960 from Creative biogene
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Human PADI4 siRNA - orb268871 from Biorbyt
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PADI4 shRNA Plasmid (Human) - CHH8369 from Cohesion Biosciences
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PADI4 miRNA 3'UTR clone - MiUTR3H-07595 from Creative biogene
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