MYLIP

Gene Symbol	MYLIP
Entrez Gene	29116
Alt Symbol	IDOL, MIR
Species	Human
Gene Type	protein-coding
Description	myosin regulatory light chain interacting protein
Other Description	E3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor\|E3 ubiquitin-protein ligase MYLIP\|band 4.1 superfamily member BZF1\|cellular modulator of immune recognition (c-MIR)\|inducible degrader of the LDL-receptor\|myosin regulatory light chain-interacting protein
Swissprots	Q9BU73 Q9NRL9 Q8WY64 Q5TIA4 Q9UHE7
Accessions	AAQ13409 EAW55366 EAW55367 Q8WY64 AF006003 AAQ13408 AF187016 AAF18974 AF212221 AAF87323 AF258586 AAG23789 AJ420601 AK026739 AK074391 AK314648 BAG37209 BC002860 AAH02860 BT007055 AAP35704 EU176184 ABW03635 HQ447323 ADQ31810 XM_005249032 XP_005249089 XM_005249033 XP_005249090 NM_013262 NP_037394
Function	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. {ECO:0000269\|PubMed:10593918, ECO:0000269\|PubMed:12826659, ECO:0000269\|PubMed:14550572, ECO:0000269\|PubMed:19520913, ECO:0000269\|PubMed:20427281, ECO:0000269\|PubMed:22109552}.
Subcellular Location	Cytoplasm {ECO:0000305\|PubMed:14550572}. Cell membrane {ECO:0000269\|PubMed:14550572}; Peripheral membrane protein {ECO:0000269\|PubMed:14550572}.
Tissue Specificity	Ubiquitously expressed. {ECO:0000269\|PubMed:10593918}.

MYLIP (C-17) - sc-240724 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

MYLIP (E-17) - sc-240722 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-MYLIP antibody - ab74562 from Abcam

Prices	$392.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-MYLIP antibody (ab74562) at 1/500 dilutionLane 1 : extracts from HeLa cells Lane 2 : extracts from Jurkat cellsLane 3 : extracts from Jurkat cells with immunising peptide at 10 µgLysates/proteins at 10 µg per lane.

Anti-MYLIP antibody - ab134994 from Abcam

Prices	$388.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MYLIP antibody (ab134994) at 1/1000 dilution + Rat kidney tissue lysate at 20 µgSecondaryDonkey anti-rabbit polyclonal IgG HRP conjugate at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MYLIP / IDOL Antibody (AP) - LS-C226901 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MYLIP / IDOL Antibody (Biotin) - LS-C226903 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MYLIP / IDOL Antibody (FITC) - LS-C226904 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MYLIP / IDOL Antibody (PE) - LS-C226906 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MYLIP / IDOL Antibody (Internal) - LS-C329864 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MYLIP / IDOL Antibody (Azide-free, HRP) - LS-C226905 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MYLIP / IDOL Antibody (aa360-409) - LS-C110763 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

MYLIP antibody LS-C110763 Western blot of SK-MEL-2 cell lysate.

Anti-MYLIP / IDOL Antibody (aa161-210) - LS-C119933 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded human brain tissue, using MYLIP Antibody. The picture on the right is treated with the synthesized peptide.

MYLIP Antibody - NBP1-56017 from Novus Biologicals

Prices	$229.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: MYLIP Antibody [NBP1-56017] - Analysis of extracts from HeLa/Jurkat cells, using MYLIP Antibody. The lane on the right is treated with the synthesized peptide.

MYLIP Antibody - NBP1-54903 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: MYLIP Antibody [NBP1-54903] - Titration: 0.2-1 ug/ml, Positive Control: SK-MEL-2 cell lysate.

MYLIP Antibody (3C10) - H00029116-M02 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3C10

MYLIP Polyclonal Antibody - bs-9674R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin embedded rat lung labeled with Anti MYLIP/Idol Polyclonal Antibody, Unconjugated (bs-9674R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Monoclonal Anti-MYLIP antibody produced in mouse - SAB1403211 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3C10

<B>ELISA</B><BR/>Monoclonal Anti-MYLIP: <B>Cat. No. SAB1403211</B>: Detection limit for recombinant GST tagged MYLIP is 0.3 ng/mL.

Anti-MYLIP antibody produced in rabbit - SAB4501317 from Sigma-Aldrich

Prices	$335.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-MYLIP: <B>Cat. No. SAB4501317</B>: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-MYLIP antibody. Cells on the left were treated with the Anti-MYLIP antibody. Cells on the right (negative control) were treated with both Anti-MYLIP antibody and the synthesized immunogen peptide.

MYLIP antibody - middle region (ARP43136_P050) - ARP43136_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

MYLIP Antibody - middle region (OAAB02732) - OAAB02732 from Aviva Systems Biology

Prices	$289.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

MYLIP-Antibody-Center - AP5315c from Abgent, a WuXi AppTec company

Prices	$99.00, $295.00
Sizes	80 µl, 400 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of lysate from mouse kidney tissue lysate, using MYLIP Antibody (Center)(Cat. #AP5315c). AP5315c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

MYLIP-Antibody-Center - AP50758 from Abgent, a WuXi AppTec company

Prices	$265.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of lysates from HepG2 cell line ,human placenta,mouse heart and rat kideny tissue lysate(from left to right),using MYLIP Antibody (Center)(AP50758). AP50758 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

MYLIP Antibody (OAAF03614) - OAAF03614 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

MYLIP antibody - orb66979 from Biorbyt

Prices	$425.00
Sizes	100 μl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of total cell extracts from human MCF7(Lane1) and mouse brain(Lane2) using MYLIP antibody

MYLIP antibody - 70R-18714 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

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