GOPC

Gene Symbol GOPC
Entrez Gene 57120
Alt Symbol CAL, FIG, GOPC1, PIST, dJ94G16.2
Species Human
Gene Type protein-coding
Description golgi-associated PDZ and coiled-coil motif containing
Other Description CFTR-associated ligand|Golgi associated PDZ and coiled-coil motif containing protein|Golgi-associated PDZ and coiled-coil motif-containing protein|PDZ protein interacting specifically with TC10|PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10|dJ94G16.2 PIST|fused in glioblastoma|golgi-associated PDZ and coiled-coil motif containing protein
Swissprots Q9HD26 Q969U8 Q59FS4 A6NM30
Accessions EAW48204 EAW48205 Q9HD26 AB209385 BAD92622 AF287894 AAG00572 AF450008 AAL47160 AJ420540 AK025074 AK314486 AY033606 AAK57733 BC009553 AAH09553 BX419592 DA109449 HQ447312 ADQ31799 KF420122 AGU13713 KF420123 AGU13714 NM_001017408 NP_001017408 NM_020399 NP_065132
Function Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. {ECO:0000269|PubMed:11707463, ECO:0000269|PubMed:14570915, ECO:0000269|PubMed:15358775}.
Subcellular Location Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cell projection, dendrite {ECO:0000250}. Note=Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. {ECO:0000250}.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:11162552, ECO:0000269|PubMed:11384996, ECO:0000269|PubMed:11707463}.

GOPC (D10A12) Rabbit mAb - 8576 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10A12
Western blot analysis of extracts from various cell lines using GOPC (D10A12) Rabbit mAb.

GOPC Antibody - 4646 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS-7 cells, untransfected or transfected with human GOPC, using GOPC Antibody.

PIST (A-7) - sc-393026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PIST (A-7): sc-393026. Western blot analysis of PIST expression in A-375 (A) and PC-3 (B) whole cell lysates.

PIST (C-15) - sc-74834 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PIST (G-19) - sc-74836 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PIST (H-134) - sc-367063 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PIST (H-134): sc-367063. Western blot analysis of PIST expression in NTERA-2 cl.D1 (A), COLO 320DM (B), A-375 (C) and PC-3 (D) whole cell lysates.

PIST (Q-23) - sc-130845 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PIST (Q-23): sc-130845. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic and membrane localization.

Anti-PIST antibody - ab37036 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PIST antibody (ab37036) at 1 µg/mlLane 2 : Anti-PIST antibody (ab37036) at 2 µg/mlLane 3 : Anti-PIST antibody (ab37036) at 4 µg/mlLane 1 : rat colon cell lysateLane 2 : rat colon cell lysateLane 3 : rat colon cell lysateLysates/proteins at 15 µg per lane.

Anti-PIST antibody - ab62563 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PIST antibody (ab62563) at 1 µg/mlLane 2 : Anti-PIST antibody (ab62563) at 2 µg/mlLane 3 : Anti-PIST antibody (ab62563) at 4 µg/mlLane 1 : PC-3 cell lysateLane 2 : PC-3 cell lysateLane 3 : PC-3 cell lysateLysates/proteins at 15 µg per lane.

Anti-PIST antibody - ab90904 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PIST antibody (ab90904) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PIST antibody - ab95067 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab95067 detecting PIST in HeLa whole cell lysate by WB following IP. Lane 1: ab95067 at 3 µg/mg of lysate Lane 2: IP with an antibody which recognizes a downstream epitope of PIST Lane 3: control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-PIST antibody [EPR4079] - ab109119 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4079
ab109119 stained HeLa cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109119 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PIST antibody [EPR4080(2)] - ab133472 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4080(2)
All lanes : Anti-PIST antibody [EPR4080(2)] (ab133472) at 1/1000 dilutionLane 1 : Human fetal kidney lysate Lane 2 : PC3 cell lysate Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PIST antibody - C-terminal - ab71191 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PIST antibody - C-terminal (ab71191) at 1/60 dilution + Ramos cell line lysates at 35 µg

Anti-GOPC / PIST Antibody (aa357-386, APC) - LS-C257877 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (aa357-386, Biotin) - LS-C257878 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (aa357-386, FITC) - LS-C257879 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (aa357-386, Azide-free, HRP) - LS-C257880 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (aa357-386, PE) - LS-C257882 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (C-Terminus, AP) - LS-C280987 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (C-Terminus, APC) - LS-C280988 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (C-Terminus, Biotin) - LS-C280989 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (C-Terminus, Azide-free, HRP) - LS-C280990 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (C-Terminus, FITC) - LS-C280991 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GOPC / PIST Antibody (Internal, AP) - LS-C280993 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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