AGO1

Gene Symbol AGO1
Entrez Gene 26523
Alt Symbol EIF2C, EIF2C1, GERP95, Q99
Species Human
Gene Type protein-coding
Description argonaute RISC catalytic component 1
Other Description Golgi Endoplasmic Reticulum protein 95 kDa|argonaute 1|argonaute1|eIF-2C 1|eIF2C 1|eukaryotic translation initiation factor 2C, 1|hAgo1|protein argonaute-1|putative RNA-binding protein Q99
Swissprots Q9UL18 Q5TA57 Q6P4S0
Accessions EAX07396 Q9UL18 AF093097 AAF00068 AK000326 AK001622 BAG50952 AK002003 AK025726 AK027611 AK125879 AK314148 BAG36835 BC063275 AAH63275 CR936823 U92986 XM_011541236 XP_011539538 XM_011541237 XP_011539539 XM_011541238 XP_011539540 XM_011541239 XP_011539541 NM_012199 NP_036331
Function Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). {ECO:0000269|PubMed:16289642, ECO:0000269|PubMed:16936728, ECO:0000269|PubMed:18771919}.
Subcellular Location Cytoplasm, P-body {ECO:0000269|PubMed:16289642, ECO:0000269|PubMed:20616046}.

Argonaute 1 (D84G10) XP ® Rabbit mAb - 5053 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D84G10
Western blot analysis of extracts from various cell types using Argonaute 1 (D84G10) XP ® Rabbit mAb.

Argonaute 1 Antibody - 9388 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Argonaute 1 Antibody.

eIF2C1 (N-16) - sc-32656 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF2C1 (N-16): sc-32656. Western blot analysis of eIF2C1 expression in HeLa (A), Hep G2 (B) and Jurkat (C) whole cell lysates.

eIF2C1 (E-12) - sc-32657 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Ago1 antibody - ab135047 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Ago1 antibody (ab135047) at 1/1000 dilution + K562 total cell extracts

Anti-Ago1 antibody [EPR10413] - ab155104 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR10413
All lanes : Anti-Ago1 antibody [EPR10413] (ab155104) at 1/10000 dilutionLane 1 : MCF7 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Ago1 antibody - Aminoterminal end - ab135577 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Ago1 antibody - Aminoterminal end (ab135577) at 1/50 dilution + K562 cell line lysates at 35 µg

Anti-AGO1 / EIF2C Antibody (aa382-395) IHC-plus™ - LS-B6465 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-EIF2C1 / AGO1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6465 concentration 2.5 ug/ml.

Anti-AGO1 / EIF2C Antibody - LS-C128713 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-AGO1 / EIF2C Antibody - LS-C128714 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AGO1 / EIF2C Antibody (clone 13A152) - LS-C183367 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 13A152

Anti-AGO1 / EIF2C Antibody (clone 11C1028) - LS-C183829 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone 11C1028

Anti-AGO1 / EIF2C Antibody (aa27-56) IHC-plus™ - LS-B10041 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-AGO1 / EIF2C antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10041 dilution 1:100.

Anti-AGO1 / EIF2C Antibody - LS-C335627 from LifeSpan Bioscience

Prices $315.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using AGO1 antibody.

Anti-AGO1 / EIF2C Antibody (N-Terminus) - LS-C107744 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EIF2C1 / AGO1 antibody LS-C107744 Western blot of THP-1 cell lysate.

Anti-Argonaute 1/2/3/4 Antibody (aa361-378) - LS-C143730 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Argonaute 1/2/3/4 Antibody (aa361-378) - LS-C148053 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

AGO1/EIF2C1 Antibody - NBP1-56530 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: AGO1/EIF2C1 Antibody [NBP1-56530] - THP-1 cell lysate, concentration 0.2-1 ug/ml.

AGO1/EIF2C1 Antibody - NB100-2817 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).

Rabbit Anti-Ago1 Polyclonal Antibody - bs-7737R from Bioss

Prices $245.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-Ago1 Polyclonal Antibody, Unconjugated (bs-7737R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-AGO1 antibody produced in rabbit - SAB4301076 from Sigma-Aldrich

Prices $230.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF2C1, (N-terminal) antibody produced in rabbit - SAB2104940 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-EIF2C1, antibody produced in rabbit: <B>Cat. No. SAB2104940</B>: Immunoblotting analysis of EIF2C1 in THP-1 cell lysate with EIF2C1 anitbody at 1 μg/mL.

Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit - SAB4200066 from Sigma-Aldrich

Prices $61.10, $360.00
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Cytoplasmic P-bodies localization of eIF2C1/Ago1 in HEK-293T cells over-expressing FLAG-tagged eIF2C1/Ago1. Cells were fixed and co-stained with 5 μg/mL Rabbit Anti-eIF2C1/Ago1 (N-Terminal) (<B>Cat. No. SAB4200066</B>) (Red) and 1:1,000 Anti-FLAG (<B>Cat. No. F3165</B>) (Green) followed by Goat Anti-Rabbit, Cy3 conjugated and Goat Anti-Mouse IgG, Atto 488 conjugated (<B>Cat. No. 62197</B>) respectively. The cells were counterstained with DAPI (Blue) to stain nuclei (×40 magnification).

Anti-EIF2C1/Ago1 antibody produced in rabbit - SAB4200065 from Sigma-Aldrich

Prices $61.10, $360.00
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Cytoplasmic P-bodies localization of eIF2C1/Ago1 in 293T cells over-expressing FLAG-tagged eIF2C1/Ago1. Cells were fixed and co-stained with 5 μg/mL Rabbit Anti-eIF2C1/Ago1 (C-terminal) (<B>Cat. No. SAB4200065</B>) (Red) and 1:1,000 Anti-FLAG (<B>Cat. No. F3165</B>) (Green) followed by Goat Anti-Rabbit, Cy3 conjugated and Goat Anti-Mouse IgG, Atto 488 conjugated (<B>Cat. No. 62197</B>) respectively. The cells were counterstained with DAPI (Blue) to stain nuclei (×40 magnification).

Monoclonal Anti-AGO1 antibody produced in rat - SAB4200084 from Sigma-Aldrich

Prices $397.50
Sizes 200 µl
Host Rat
Clonality Monoclonal
Clone 4B8
<B>Immunoblotting</B><BR/>Lysates were separated on SDS-PAGE and probed with 1 μg/mL Rat Anti-AGO1 Clone: 4B8 (<B>Cat. No. SAB4200084</B>). The antibody was developed using Rabbit Anti-RAT IgG-Peroxidase (<B>Cat. No. A9542</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 293T; 2. Neuro 2a; 3. L8; 4. CFB; 5. CHO; 6. MDBK; 7. MDCK; 8. COS7
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