TRIM63

Gene Symbol TRIM63
Entrez Gene 84676
Alt Symbol IRF, MURF1, MURF2, RNF28, SMRZ
Species Human
Gene Type protein-coding
Description tripartite motif containing 63, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase TRIM63|iris ring finger protein|muscle specific ring finger protein 2|muscle-specific RING finger protein 1|ring finger protein 28|striated muscle RING zinc finger protein|tripartite motif-containing protein 63
Swissprots Q9BYV4 B4DN95 Q96KD9 Q5T2I1 Q969Q1 Q96BD3
Accessions EAX07848 Q969Q1 AF353673 AAK39519 AF361946 AAK52497 AI351421 AJ276484 CAC81706 AK056942 BAB71318 AK297820 BAG60157 BC080529 AAH80529 BT007373 AAP36037 DA900348 NM_032588 NP_115977
Function E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti- hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. {ECO:0000269|PubMed:11927605, ECO:0000269|PubMed:18222470}.
Subcellular Location Cytoplasm. Nucleus {ECO:0000250}. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle. {ECO:0000250}.
Tissue Specificity Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris. {ECO:0000269|PubMed:11243782, ECO:0000269|PubMed:11283016, ECO:0000269|PubMed:11679633, ECO:0000269|PubMed:12107412}.

MURF1 Antibody - 4305 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7, Hep G2 and HeLa cells using MURF1 Antibody.

MuRF1 (C-11) - sc-398608 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MuRF1 (C-11): sc-398608. Western blot analysis of MuRF1 expression in non-transfected: sc-117752 (A) and human MuRF1 transfected: sc-369006 (B) 293T whole cell lysates and mouse heart (C) and mouse skeletal muscle (D) tissue extracts.

MuRF1 (C-20) - sc-27642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MuRF1 (C-20): sc-27642. Western blot analysis of MuRF1 expression in HeLa whole cell lysate.

MuRF1 (N-15) - sc-27640 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MuRF1 (H-145) - sc-32920 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MuRF1 (H-145): sc-32920. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic localization.

MuRF1 (SW-53) - sc-134397 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MuRF1 (SW-53): sc-134397. Western blot analysis of MuRF1 expression in human MuRF1 transfected (A) and non-transfected (B) 293T whole cell lysates.

Anti-MURF1 antibody - ab4125 from Abcam

Prices $382.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab4125 at 1.25 µg/ml staining human skeletal muscle by IHC-P.  Tissue sections were subjected to steamed antigen retrieval with citrate buffer (pH6), AP-staining.  Shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.

Anti-MURF1 antibody - ab77577 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MURF1 antibody (ab77577) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Heart (Mouse) Tissue LysateLane 3 : Skeletal Muscle (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MURF1 antibody - ab183094 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MURF1 antibody (ab183094) at 1/1050 dilution + lysate at 40 µgSecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/5000 dilutiondeveloped using the ECL technique

Anti-MURF1 antibody - ab96857 from Abcam

Prices $387.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-MURF1 antibody (ab96857) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-MURF1 antibody - ab57865 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57865 MURF1 antibody at 1ug/ml.

Anti-MURF1 antibody - ab172901 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MURF1 antibody (ab172901) at 1 µg/mlLane 1 : MURF1-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-MURF1 antibody [7B8E1] - ab201941 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7B8E1
Immunofluorescent analysis of HeLa cells labeling MURF1 with ab201941 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Anti-MURF1 antibody [EPR6431(2)] - ab172479 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6431(2)
All lanes : Anti-MURF1 antibody [EPR6431(2)] (ab172479) at 1/1000 dilutionLane 1 : Rat muscle tissue lysateLane 2 : Mouse muscle tissue lysateLane 3 : Human skeletal muscle tissue lysateLysates/proteins at 10 µg per lane.

Anti-MURF1 / IRF Antibody (aa251-300) - LS-C110780 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TRIM63 / MURF1 antibody LS-C110780 Western blot of THP-1 cell lysate.

Anti-MURF1 / IRF Antibody - LS-C332362 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human esophagus cancer tissue using TRIM63 antibody.

Anti-MURF1 / IRF Antibody (aa2-14) IHC-plus™ - LS-B1751 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-TRIM63 / MURF1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1751 concentration 25 ug/ml.

Anti-MURF1 / IRF Antibody (aa247-261) IHC-plus™ - LS-B1750 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-TRIM63 / MURF1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1750 concentration 25 ug/ml.

Anti-MURF1 / IRF Antibody (aa1-44) - LS-C186154 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Breast ca using MURF1 antibody at 1:500 dilution.

Anti-MURF1 / IRF Antibody (aa186-235) - LS-C205733 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

MuRF1/TRIM63 Antibody - NBP1-31207 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody diluted by 1:1000.

MuRF1/TRIM63 Antibody - H00084676-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MURF1 Antibody [H00084676-D01] - Expression in human stomach.

MuRF1/TRIM63 Antibody - NBP1-54939 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MuRF1/TRIM63 Antibody [Unconjugated] - AF5366 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MuRF1 at approximately 41‑44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MuRF1/TRIM63 Antibody - NB100-1193 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-1193] - (1.25ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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