GPNMB

Gene Symbol GPNMB
Entrez Gene 10457
Alt Symbol HGFIN, NMB
Species Human
Gene Type protein-coding
Description glycoprotein (transmembrane) nmb
Other Description glycoprotein NMB|glycoprotein nmb-like protein|osteoactivin|transmembrane glycoprotein HGFIN|transmembrane glycoprotein NMB
Swissprots A4D155 Q6UVX1 Q8N1A1 Q14956
Accessions AAN59897 AAP22336 AAP22337 EAL24259 EAW93781 EAW93782 EAW93783 EAW93784 Q14956 AF322909 AAG42839 AI952217 AJ505015 CAD43718 AK292078 BAF84767 AK296779 BAG59357 AK297031 BAG59559 AK297051 BAG59577 AK297055 BAG59580 AK297803 BAG60141 AK303071 BAG64187 AK304671 BAG65446 AK312279 BAG35208 AY359124 AAQ89481 BC011595 AAH11595 BC025297 BC032783 AAH32783 BM750843 BT007074 AAP35737 BU537639 CD105306 DQ892084 ABM83010 DQ895277 ABM86203 X76534 CAA54044 XM_005249578 XP_005249635 NM_001005340 NP_001005340 NM_002510 NP_002501
Function Could be a melanogenic enzyme. {ECO:0000250}.
Subcellular Location Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected at the cell surface in different types of cancers cells, including glioblastoma multiforme cells and most melanoma cell lines.
Tissue Specificity Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain. {ECO:0000269|PubMed:16489096, ECO:0000269|PubMed:16609006, ECO:0000269|PubMed:7814155}.

GPNMB (E1Y7J) Rabbit mAb - 13251 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1Y7J
Western blot analysis of extracts from A172 and SK-MEL-28 cells using GPNMB (E1Y7J) Rabbit mAb.

GPNMB (G-8) - sc-271416 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPNMB (G-8): sc-271416. Western blot analysis of GPNMB expression in SK-MEL-28 whole cell lysate.

GPNMB (D-9) - sc-271415 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPNMB (D-9): sc-271415. Western blot analysis of GPNMB expression in SK-MEL-28 whole cell lysate.

GPNMB (N-15) - sc-47008 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

GPNMB (H-121) - sc-99091 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GPNMB (H-121): sc-99091. Western blot analysis of GPNMB expression in mouse liver tissue extract.

GPNMB (K-16) - sc-47006 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GPNMB (K-16): sc-47006. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and Islets of Langerhans.

GPNMB (M-90) - sc-99090 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GPNMB (M-90): sc-99090. Western blot analysis of GPNMB expression in mouse liver tissue extract.

GPNMB (S-24) - sc-133634 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal

Anti-GPNMB antibody - ab125898 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab125898 stained Malme-3m cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab125898 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-GPNMB antibody - ab98856 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GPNMB antibody (ab98856) at 1 µg/ml + PANC1 cell lysate at 10 µg

Anti-GPNMB antibody - ab56584 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56584 GPNMB antibody at 1ug/ml.

Anti-GPNMB antibody - ab130622 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GPNMB antibody (ab130622) at 1/100 dilution + CEM cell line lysate at 35 µg

Anti-GPNMB antibody [7C10E5] - ab175427 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7C10E5
All lanes : Anti-GPNMB antibody [7C10E5] (ab175427) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : GPNMB (AA: 31-260)-hIgGFc transfected HEK293 cell lysate

Anti-GPNMB / Osteoactivin Antibody (aa50-99) IHC-plus™ - LS-B11132 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GPNMB antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-GPNMB / Osteoactivin Antibody (aa551-568) - LS-C131166 from LifeSpan Bioscience

Prices $405.00
Sizes 20 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (aa74-123) - LS-C80663 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-GPNMB / Osteoactivin Antibody (aa541-569, AP) - LS-C257943 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (aa541-569, APC) - LS-C257944 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (aa541-569, Biotin) - LS-C257945 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (aa541-569, FITC) - LS-C257946 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (aa541-569, Azide-free, HRP) - LS-C257947 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (aa541-569, PE) - LS-C257949 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (clone 3A5) IHC-plus™ - LS-B6406 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3A5
Anti-GPNMB antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6406 concentration 5 ug/ml.

Anti-GPNMB / Osteoactivin Antibody (aa540-569) - LS-C321729 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPNMB / Osteoactivin Antibody (aa104-203) - LS-C307741 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
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