ALKBH8

Gene Symbol ALKBH8
Entrez Gene 91801
Alt Symbol ABH8
Species Human
Gene Type protein-coding
Description alkB, alkylation repair homolog 8 (E. coli)
Other Description AlkB homologue 8|S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8|alkylated DNA repair protein alkB homolog 8|probable alpha-ketoglutarate-dependent dioxygenase ABH8|tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8
Swissprots Q96BT7 Q8N989 B4DEF6 B1Q2M0
Accessions EAW67088 EAW67089 EAW67090 Q96BT7 AB218768 BAG16270 AF086489 AK095523 BAC04566 AK293603 BAG57067 AK298800 BAG60935 AK304413 BAG65244 BC015183 AAH15183 BX649085 HQ447168 ADQ31655 XM_005271728 XP_005271785 XM_005271729 XP_005271786 XM_005271730 XP_005271787 XM_005271732 XP_005271789 XM_011543067 XP_011541369 NM_001301010 NP_001287939 NM_138775 NP_620130
Function Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182). {ECO:0000269|PubMed:19293182, ECO:0000269|PubMed:20123966, ECO:0000269|PubMed
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17979886, ECO:0000269|PubMed:20308323}. Nucleus {ECO:0000269|PubMed:20308323}. Note=Predominantly cytoplasmic. {ECO:0000269|PubMed:20308323}.
Tissue Specificity Widely expressed, with highest expression in spleen, followed by pancreas and lung. {ECO:0000269|PubMed:17979886}.

ALKBH8-Over-expression-Lysate - LY10748a from Abgent, a WuXi AppTec company

Category Cell Lysate
Prices $325.00
Sizes 100 µg
Western blot analysis of his-tag ALKBH8 (arrow) using his tag antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALKBH8 gene

ALKBH8 overexpression lysate - LY432343 from Acris Antibodies

Category Cell Lysate
Prices $280.00
Sizes 100 µg

ALKBH8 Overexpression Lysate (Native) - NBP2-10239 from Novus Biologicals

Category Cell Lysate
Prices $329.00
Sizes 100 µg
Western Blot: ALKBH8 Overexpression Lysate (Adult Normal) [NBP2-10239] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALKBH8.

Panoply™ Human ALKBH8 Knockdown Stable Cell Line - CSC-DC000518 from Creative biogene

Category Cell Line
Species Human
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