ALKBH8

Gene Symbol ALKBH8
Entrez Gene 91801
Alt Symbol ABH8
Species Human
Gene Type protein-coding
Description alkB, alkylation repair homolog 8 (E. coli)
Other Description AlkB homologue 8|S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8|alkylated DNA repair protein alkB homolog 8|probable alpha-ketoglutarate-dependent dioxygenase ABH8|tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8
Swissprots Q96BT7 Q8N989 B4DEF6 B1Q2M0
Accessions EAW67088 EAW67089 EAW67090 Q96BT7 AB218768 BAG16270 AF086489 AK095523 BAC04566 AK293603 BAG57067 AK298800 BAG60935 AK304413 BAG65244 BC015183 AAH15183 BX649085 HQ447168 ADQ31655 XM_005271728 XP_005271785 XM_005271729 XP_005271786 XM_005271730 XP_005271787 XM_005271732 XP_005271789 XM_011543067 XP_011541369 NM_001301010 NP_001287939 NM_138775 NP_620130
Function Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182). {ECO:0000269|PubMed:19293182, ECO:0000269|PubMed:20123966, ECO:0000269|PubMed
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17979886, ECO:0000269|PubMed:20308323}. Nucleus {ECO:0000269|PubMed:20308323}. Note=Predominantly cytoplasmic. {ECO:0000269|PubMed:20308323}.
Tissue Specificity Widely expressed, with highest expression in spleen, followed by pancreas and lung. {ECO:0000269|PubMed:17979886}.

ALKBH8 (P-16) - sc-101882 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
ALKBH8 (P-16): sc-101882. Western blot analysis of ALKBH8 expression in Jurkat whole cell lysate.

Anti-ALKBH8 antibody - ab113512 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ALKBH8 antibody (ab113512) at 1/500 dilutionLane 1 : Human fetal spleen lysateLane 2 : A549 cell lysate

Anti-ALKBH8 antibody - ab91482 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ALKBH8 antibody (ab91482) at 1/50 dilution + HL60 cell line lysate at 35 µg

Anti-ALKBH8 Antibody (aa494-523) - LS-C156285 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-ALKBH8 Antibody (aa141-190) - LS-C30641 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: Jurkat Cell Lysate

Anti-ALKBH8 Antibody (N-Terminus) - LS-C80398 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.

Anti-ALKBH8 Antibody (aa2-238) - LS-C178529 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ALKBH8 Antibody - LS-C346248 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of A549 cell using ALKBH8 antibody. Blue: DAPI for nuclear staining.

ALKBH8 Antibody - NBP1-81238 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ALKBH8 Antibody [NBP1-81238] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

ALKBH8 Antibody - NBP2-38928 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: ALKBH8 Antibody [NBP2-38928] - Staining of human cell line U-2 OS shows positivity in nucleus.

ALKBH8 Antibody - NBP1-80474 from Novus Biologicals

Prices $139.00, $299.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ALKBH8 Antibody [NBP1-80474] - Jurkat cell lysate, concentration 1.0ug/ml.

ALKBH8 Antibody - NBP1-80473 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ALKBH8 Antibody [NBP1-80473] - Human Liver cell lysate, concentration 0.2-1 ug/ml.

ALKBH8 Antibody - NBP2-38428 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ABH8 Polyclonal Antibody - bs-6510R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ALKBH8 antibody produced in rabbit - AV41107 from Sigma-Aldrich

Prices $267.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: Jurkat (<B>Cat. No. AV41107</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.0 μg/mL in cell type Jurkat

Anti-ALKBH8 antibody produced in rabbit - AV41106 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: liver (<B>Cat. No. AV41106</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.5 μg/mL in cell type liver

Anti-ALKBH8 antibody produced in rabbit - HPA038725 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ALKBH8: <B>Cat. No. HPA038725</B>: Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.

ALKBH8 antibody - N-terminal region (ARP41106_P050) - ARP41106_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ALKBH8 antibody - N-terminal region (ARP41107_T100) - ARP41107_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ALKBH8-Antibody-C-term - AP6801b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ALKBH8 Antibody - C-terminal region (OAAB03896) - OAAB03896 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

ALKBH8 antibody - GTX122886 from GeneTex

Host Rabbit
Clonality Polyclonal

ALKBH8 antibody, C-term - GTX81711 from GeneTex

Prices $319.00
Sizes 400 μl
Host Rabbit
Clonality Polyclonal
Anti-ALKBH8 antibody, C-term used in Flow cytometry/FACS. GTX81711

ALKBH8 antibody, N-term - GTX47220 from GeneTex

Prices $319.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Anti-ALKBH8 antibody, N-term used in Western blot. GTX47220

ALKBH8 antibody, N-term - GTX47221 from GeneTex

Prices $319.00
Sizes 50 μg
Host Rabbit
Clonality Polyclonal
Anti-ALKBH8 antibody, N-term used in Western blot. GTX47221
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