ALKBH8
| Gene Symbol | ALKBH8 |
|---|---|
| Entrez Gene | 91801 |
| Alt Symbol | ABH8 |
| Species | Human |
| Gene Type | protein-coding |
| Description | alkB, alkylation repair homolog 8 (E. coli) |
| Other Description | AlkB homologue 8|S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8|alkylated DNA repair protein alkB homolog 8|probable alpha-ketoglutarate-dependent dioxygenase ABH8|tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 |
| Swissprots | Q96BT7 Q8N989 B4DEF6 B1Q2M0 |
| Accessions | EAW67088 EAW67089 EAW67090 Q96BT7 AB218768 BAG16270 AF086489 AK095523 BAC04566 AK293603 BAG57067 AK298800 BAG60935 AK304413 BAG65244 BC015183 AAH15183 BX649085 HQ447168 ADQ31655 XM_005271728 XP_005271785 XM_005271729 XP_005271786 XM_005271730 XP_005271787 XM_005271732 XP_005271789 XM_011543067 XP_011541369 NM_001301010 NP_001287939 NM_138775 NP_620130 |
| Function | Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182). {ECO:0000269|PubMed:19293182, ECO:0000269|PubMed:20123966, ECO:0000269|PubMed |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:17979886, ECO:0000269|PubMed:20308323}. Nucleus {ECO:0000269|PubMed:20308323}. Note=Predominantly cytoplasmic. {ECO:0000269|PubMed:20308323}. |
| Tissue Specificity | Widely expressed, with highest expression in spleen, followed by pancreas and lung. {ECO:0000269|PubMed:17979886}. |
ALKBH8 CRISPR/Cas9 KO Plasmid (h) - sc-407123 from Santa Cruz Biotechnology
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ALKBH8 HDR Plasmid (h) - sc-407123-HDR from Santa Cruz Biotechnology
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ALKBH8 Double Nickase Plasmid (h) - sc-407123-NIC from Santa Cruz Biotechnology
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ALKBH8 Double Nickase Plasmid (h2) - sc-407123-NIC-2 from Santa Cruz Biotechnology
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ALKBH8 CRISPR Activation Plasmid (h) - sc-407123-ACT from Santa Cruz Biotechnology
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ALKBH8 CRISPR Activation Plasmid (h2) - sc-407123-ACT-2 from Santa Cruz Biotechnology
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ALKBH8 Lentiviral Activation Particles (h) - sc-407123-LAC from Santa Cruz Biotechnology
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ALKBH8 Lentiviral Activation Particles (h2) - sc-407123-LAC-2 from Santa Cruz Biotechnology
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ALKBH8 siRNA (h) - sc-96576 from Santa Cruz Biotechnology
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ALKBH8 shRNA Plasmid (h) - sc-96576-SH from Santa Cruz Biotechnology
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ALKBH8 shRNA (h) Lentiviral Particles - sc-96576-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU086761 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3073 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3073 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR05536 from Sigma-Aldrich
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ALKBH8 siRNA (Human) - CRJ4081 from Cohesion Biosciences
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ALKBH8 RNAi - H00091801-R01 from Novus Biologicals
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ALKBH8 - MBS8239507 from MyBioSource
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shRNA set against Human ALKBH8 (NM_138775.2) - SHH235294 from Creative biogene
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ALKBH8 shRNA Plasmid (Human) - CHJ4081 from Cohesion Biosciences
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ALKBH8 miRNA 3'UTR clone - MiUTR3H-12831 from Creative biogene
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