VCL

Gene Symbol VCL
Entrez Gene 7414
Alt Symbol CMD1W, CMH15, HEL114, MV, MVCL
Species Human
Gene Type protein-coding
Description vinculin
Other Description epididymis luminal protein 114|metavinculin
Swissprots Q8IXU7 Q16450 Q7Z3B8 P18206 Q5SWX2
Accessions AAA61271 AAB21656 AAB21657 EAW54547 EAW54548 EAW54549 EAW54550 EAW54551 P18206 AA368699 AI290052 AK126978 BAG54414 AK296509 BAG59141 AK300280 BAG62039 AK304823 BAG65571 AL700886 AL708715 AL832130 BC013847 BC039174 AAH39174 BX537994 CAD97952 DQ894680 ABM85606 EU794673 ACJ13727 M33308 AAA61283 XM_005270142 XP_005270199 XM_005270143 XP_005270200 NM_003373 NP_003364 NM_014000 NP_054706
Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. {ECO:0000269|PubMed:20484056}.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000269|PubMed:18439753}. Cell junction, adherens junction {ECO:0000269|PubMed:18439753}. Cell membrane {ECO:0000269|PubMed:18439753}; Peripheral membrane protein {ECO:0000269|PubMed:18439753}; Cytoplasmic side {ECO:0000269|PubMed:18439753}. Cell junction, focal adhesion {ECO:0000269|PubMed:18439753}. Note=Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions. {ECO:0000250}.
Tissue Specificity Metavinculin is muscle-specific.
Top Pathways Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Bacterial invasion of epithelial cells, Amoebiasis

Vinculin (E1E9V) XP ® Rabbit mAb - 13901 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1E9V
Western blot analysis of extracts from various cell lines using Vinculin (E1E9V) XP ® Rabbit mAb (upper) or α-Actinin (D6F6) XP ® Rabbit mAb, #6487 (lower).

Vinculin Antibody - 4650 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell and tissue types using Vinculin Antibody.

vinculin (7F9) - sc-73614 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
vinculin (7F9): sc-73614. Western blot analysis of vinculin expression in HeLa (A), K-562 (B), U-937 (C) and PC-3 (D) whole cell lysates.

vinculin (C-20) - sc-7648 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
vinculin (C-20): sc-7648. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

vinculin (G-11) - sc-55465 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
vinculin (G-11): sc-55465. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

vinculin (H-10) - sc-25336 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
vinculin (H-10): sc-25336. Western blot analysis of vinculin expression in MDCK (A) and K-562 (B) whole cell lysates.

vinculin (H-300) - sc-5573 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
vinculin (H-300): sc-5573. Western blot analysis of vinculin expression in HUV-EC-C (A) and HISM (B) whole cell lysates.

vinculin (N-19) - sc-7649 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
vinculin (N-19): sc-7649. Western blot analysis of vinculin expression in HUV-EC-C (A), HeLa (B), MDCK (C) and HISM (D) whole cell lysates.

vinculin (V284) - sc-59803 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
vinculin (V284): sc-59803. Western blot analysis of vinculin expression in HeLa (A), K-562 (B), U-937 (C) and PC-3 (D) whole cell lysates.

vinculin (2Q1087) - sc-73264 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
vinculin (2Q1087): sc-73264. Western blot analysis of vinculin expression in non-transfected 293T: sc-117752 (A), human vinculin transfected 293T: sc-113822 (B) and HUV-EC-C (C) whole cell lysates.

Anti-Vinculin (phospho Y821) antibody - ab195758 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Vinculin (phospho Y821) antibody (ab195758) at 1/500 dilutionLane 1 : forskolin treated HeLa whole cell lysates with antigen-specific peptideLane 2 : forskolin treated HeLa whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Vinculin (phospho Y821) antibody - ab61071 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab61071 at 1/50-1/100 dilution with or without immunising phosphopeptide.

Anti-Vinculin antibody - ab61186 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab61186 at 1/50 dilution. Samples were treated -/+ immunising peptide.

Anti-Vinculin antibody - ab119278 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab119278, at 10 ug/ml, staining Vinculin in formalin-fixed, paraffin-embedded Human heart tissue by Immunohistochemistry.

Anti-Vinculin antibody - ab82373 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Vinculin antibody (ab82373) at 1/50 dilution + Ramos cell line lysate at 35 µg

Anti-Vinculin antibody [EPR8185] (Alexa Fluor® 488) - ab196454 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8185
ab196454 staining Vinculin in HUVEC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196454 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HUVEC cells fixed with 100% methanol (5min).

Anti-Vinculin antibody [EPR8185] (Alexa Fluor® 647) - ab196579 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8185
ab196579 staining Vinculin in HUVEC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196579 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HUVEC cells fixed with 4% formaldehyde (10 min).

Anti-Vinculin antibody [EPR8185] - Loading Control - ab129002 from Abcam

Prices $379.00, $759.00
Sizes 100 µl, 250 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8185
All lanes : Anti-Vinculin antibody [EPR8185] - Loading Control (ab129002) at 1/10000 dilutionLane 1 : PC3 cell lysateLane 2 : HeLa cell lysateLane 3 : U937 cell lysateLane 4 : K562 cell lysateLane 5 : HUVEC cell lysateLane 6 : Human fetal liver lysateLane 7 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-Vinculin antibody [SP117] - ab111004 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP117
ab111004 at 1/100 dilution staining Vinculin in Formalin-fixed, paraffin-embedded Human heart tissue.

Anti-Vinculin antibody [SPM227] - ab18058 from Abcam

Prices $404.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone SPM227
ab18058 at 1/200 staining Chinese Hamster Ovary cells by ICC/IF. The cells were permeabilized with 100% methanol and then blocked with 1% BSA/ 4% goat serum prior to incubation with the antibody for 30 minutes. A rhodamine conjugated goat antibody was used as the secondary.See Abreview

Anti-Vinculin antibody [SPM227], prediluted - ab54195 from Abcam

Prices $370.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone SPM227

Anti-Vinculin antibody [VLN01] - ab80665 from Abcam

Prices $373.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone VLN01

Anti-Vinculin antibody - N-terminal - ab82418 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Vinculin antibody - N-terminal (ab82418) at 1/50 dilutionLane 1 : K562 cell lysateLane 2 : Mouse lung tissue lysateLysates/proteins at 35 µg per lane.

Anti-VCL / Vinculin Antibody (C-Terminus, clone SP117) - LS-C210362 from LifeSpan Bioscience

Prices $520.00
Sizes 500 µl
Host Rabbit
Clonality Monoclonal
Clone SP117
Immunohistochemistry of Human Stomach stained with anti-Vinculin antibody

Anti-VCL / Vinculin Antibody (clone MaVinc) - LS-C11489 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone MaVinc
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