VCL
| Gene Symbol | VCL |
|---|---|
| Entrez Gene | 7414 |
| Alt Symbol | CMD1W, CMH15, HEL114, MV, MVCL |
| Species | Human |
| Gene Type | protein-coding |
| Description | vinculin |
| Other Description | epididymis luminal protein 114|metavinculin |
| Swissprots | Q8IXU7 Q16450 Q7Z3B8 P18206 Q5SWX2 |
| Accessions | AAA61271 AAB21656 AAB21657 EAW54547 EAW54548 EAW54549 EAW54550 EAW54551 P18206 AA368699 AI290052 AK126978 BAG54414 AK296509 BAG59141 AK300280 BAG62039 AK304823 BAG65571 AL700886 AL708715 AL832130 BC013847 BC039174 AAH39174 BX537994 CAD97952 DQ894680 ABM85606 EU794673 ACJ13727 M33308 AAA61283 XM_005270142 XP_005270199 XM_005270143 XP_005270200 NM_003373 NP_003364 NM_014000 NP_054706 |
| Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. {ECO:0000269|PubMed:20484056}. |
| Subcellular Location | Cytoplasm, cytoskeleton {ECO:0000269|PubMed:18439753}. Cell junction, adherens junction {ECO:0000269|PubMed:18439753}. Cell membrane {ECO:0000269|PubMed:18439753}; Peripheral membrane protein {ECO:0000269|PubMed:18439753}; Cytoplasmic side {ECO:0000269|PubMed:18439753}. Cell junction, focal adhesion {ECO:0000269|PubMed:18439753}. Note=Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions. {ECO:0000250}. |
| Tissue Specificity | Metavinculin is muscle-specific. |
| Top Pathways | Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Bacterial invasion of epithelial cells, Amoebiasis |
vinculin CRISPR/Cas9 KO Plasmid (h) - sc-400227 from Santa Cruz Biotechnology
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vinculin HDR Plasmid (h) - sc-400227-HDR from Santa Cruz Biotechnology
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vinculin Double Nickase Plasmid (h) - sc-400227-NIC from Santa Cruz Biotechnology
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vinculin Double Nickase Plasmid (h2) - sc-400227-NIC-2 from Santa Cruz Biotechnology
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vinculin CRISPR Activation Plasmid (h) - sc-400227-ACT from Santa Cruz Biotechnology
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vinculin CRISPR Activation Plasmid (h2) - sc-400227-ACT-2 from Santa Cruz Biotechnology
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vinculin Lentiviral Activation Particles (h) - sc-400227-LAC from Santa Cruz Biotechnology
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vinculin Lentiviral Activation Particles (h2) - sc-400227-LAC-2 from Santa Cruz Biotechnology
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vinculin siRNA (h) - sc-29524 from Santa Cruz Biotechnology
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vinculin shRNA Plasmid (h) - sc-29524-SH from Santa Cruz Biotechnology
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vinculin shRNA (h) Lentiviral Particles - sc-29524-V from Santa Cruz Biotechnology
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Vinculin RNAi - H00007414-R02 from Novus Biologicals
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Vinculin RNAi - H00007414-R01 from Novus Biologicals
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MISSION® esiRNA - EHU088101 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22655 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22655 from Sigma-Aldrich
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VCL siRNA (Human) - CRH5072 from Cohesion Biosciences
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VCL - MBS8226754 from MyBioSource
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shRNA set against Human VCL (NM_003373.3) - SHH442444 from Creative biogene
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shRNA set against Human VCL(NM_003373.3) - SHL121846 from Creative biogene
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shRNA set against Human VCL(NM_014000.2) - SHL122194 from Creative biogene
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shRNA set against Human VCL(NM_003373.3) - SHL122230 from Creative biogene
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Human VCL siRNA - orb265574 from Biorbyt
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VCL shRNA Plasmid (Human) - CHH5072 from Cohesion Biosciences
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VCL miRNA 3'UTR clone - MiUTR1H-11104 from Creative biogene
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