EED

Gene Symbol EED
Entrez Gene 8726
Alt Symbol HEED, WAIT1
Species Human
Gene Type protein-coding
Description embryonic ectoderm development
Other Description WD protein associating with integrin cytoplasmic tails 1|polycomb protein EED
Swissprots Q6NTH2 A8K7V5 Q86VV2 Q7LDG8 Q7LDA5 Q9UNY7 O75530 O00149
Accessions CCQ43235 EAW75129 EAW75130 EAW75131 O75530 AA811323 AF070418 AAC23685 AF078933 AAC68675 AF080227 AAC95144 AF099032 AAD08815 AI378297 AK292120 BAF84809 AK294133 BAG57461 AK301265 BAG62829 AL546955 AW263732 BC047672 AAH47672 BC062443 BC068995 AAH68995 DB086430 EU446743 ABZ92272 U90651 AAD08714 XM_005274373 XP_005274430 XM_011545329 XP_011543631 XM_011545330 XP_011543632 XM_011545331 XP_011543633 XR_247215 NM_001308007 NP_001294936 NM_003797 NP_003788 NM_152991 NP_694536
Function Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. {ECO:0000269|PubMed:10581039, ECO:0000269|PubMed:14532106, ECO:0000269|PubMed:15225548, ECO:0000269|PubMed:15231737, ECO:0000269|PubMed:15385962, ECO:0000269|PubMed:16357870, ECO:0000269|PubMed:18285464, ECO:0000269|PubMed:20974918, ECO:0000269|PubMed:9584199}.
Subcellular Location Nucleus. Chromosome. Note=Transiently colocalizes with XIST at inactive X chromosomes.
Tissue Specificity Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. {ECO:0000269|PubMed:15684044, ECO:0000269|PubMed:9584199, ECO:0000269|PubMed:9765275, ECO:0000269|PubMed:9806832, ECO:0000269|PubMed:9880543}.

EED (H-300) - sc-28701 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
EED (H-300): sc-28701. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear and nucleolar localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

EED (V-20) - sc-18456 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EED (V-20): sc-18456. Western blot analysis of EED expression in HL-60 (A), K-562 (B) and U-2 OS (C) whole cell lysates and K-562 (D) and HL-60 (E) nuclear extracts.

EED (A-20) - sc-30812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EED (A-20): sc-30812. Western blot analysis of EED expression in mouse embryo tissue extract.

EED (E-15) - sc-30811 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

EED (U-22) - sc-133537 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
EED (U-22): sc-133537. Western blot analysis of EED expression in Hep G2 whole cell lysate.

Anti-EED antibody [163C] - ab126542 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 163C
Immunohistochemical analysis of paraffin embedded Human lymph node (top left), thymus (top right), testicle (bottom left) and bone marrow (bottom right) tissues labeling EED with ab126542 antibody at 1/600 dilution.

Anti-EED Antibody - LS-C334018 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using EED antibody.

Anti-EED Antibody (aa1-229) - LS-C186255 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded SAS Xenograft using EED antibody at 1:100 dilution.

Anti-EED Antibody (N-Terminus) - LS-C153641 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of EED in human heart tissue with EED antibody at 20 ug/ml.

Anti-EED Antibody - LS-C125507 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-EED Antibody (aa429-441) - LS-C125506 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EED Antibody (aa2-150) - LS-C125508 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-EED Antibody - LS-C307900 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-EED Antibody (aa21-70) - LS-C30407 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HepG2 Cell Lysate

Anti-EED Antibody (aa342-441) - LS-C307901 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-EED Antibody (aa35-84) - LS-C107772 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EED antibody LS-C107772 Western blot of OVCAR-3 cell lysate.

Anti-EED Antibody (C-Terminus) - LS-C145799 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EED antibody LS-C145799 Western blot of Rat Muscle lysate. Antibody concentration 1 ug/ml.

Anti-EED Antibody - LS-C341596 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

EED Antibody - AF5827 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HT‑29 human colon adenocarcinoma cell line, human colon cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EED Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5827) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EED at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

EED Antibody - NBP1-33417 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

EED Antibody (671) - NBP2-14940 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 671
Western Blot: EED Antibody (671) [NBP2-14940] - A. 30 ug Rat-2 whole cell lysate/extract 10 % SDS-PAGEEED antibody [671] dilution: 1:1000

EED Antibody - NBP2-38907 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

EED Antibody - H00008726-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: EED Antibody [H00008726-B01P] - Analysis of EED expression in transfected 293T cell line by EED polyclonal antibody. Lane 1: EED transfected lysate(48.51 KDa). Lane 2: Non-transfected lysate.

EED Antibody (GT1679) - NBP2-14939 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT1679
Western Blot: EED Antibody (GT1679) [NBP2-14939] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE; antibody diluted at 1:10000.

EED Antibody (3B12) - H00008726-M05 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B12
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