Gene Symbol | EED |
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Entrez Gene | 8726 |
Alt Symbol | HEED, WAIT1 |
Species | Human |
Gene Type | protein-coding |
Description | embryonic ectoderm development |
Other Description | WD protein associating with integrin cytoplasmic tails 1|polycomb protein EED |
Swissprots | Q6NTH2 A8K7V5 Q86VV2 Q7LDG8 Q7LDA5 Q9UNY7 O75530 O00149 |
Accessions | CCQ43235 EAW75129 EAW75130 EAW75131 O75530 AA811323 AF070418 AAC23685 AF078933 AAC68675 AF080227 AAC95144 AF099032 AAD08815 AI378297 AK292120 BAF84809 AK294133 BAG57461 AK301265 BAG62829 AL546955 AW263732 BC047672 AAH47672 BC062443 BC068995 AAH68995 DB086430 EU446743 ABZ92272 U90651 AAD08714 XM_005274373 XP_005274430 XM_011545329 XP_011543631 XM_011545330 XP_011543632 XM_011545331 XP_011543633 XR_247215 NM_001308007 NP_001294936 NM_003797 NP_003788 NM_152991 NP_694536 |
Function | Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. {ECO:0000269|PubMed:10581039, ECO:0000269|PubMed:14532106, ECO:0000269|PubMed:15225548, ECO:0000269|PubMed:15231737, ECO:0000269|PubMed:15385962, ECO:0000269|PubMed:16357870, ECO:0000269|PubMed:18285464, ECO:0000269|PubMed:20974918, ECO:0000269|PubMed:9584199}. |
Subcellular Location | Nucleus. Chromosome. Note=Transiently colocalizes with XIST at inactive X chromosomes. |
Tissue Specificity | Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. {ECO:0000269|PubMed:15684044, ECO:0000269|PubMed:9584199, ECO:0000269|PubMed:9765275, ECO:0000269|PubMed:9806832, ECO:0000269|PubMed:9880543}. |
EED CRISPR/Cas9 KO Plasmid (h) - sc-401234 from Santa Cruz Biotechnology
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EED HDR Plasmid (h) - sc-401234-HDR from Santa Cruz Biotechnology
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EED Double Nickase Plasmid (h) - sc-401234-NIC from Santa Cruz Biotechnology
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EED Double Nickase Plasmid (h2) - sc-401234-NIC-2 from Santa Cruz Biotechnology
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EED CRISPR Activation Plasmid (h) - sc-401234-ACT from Santa Cruz Biotechnology
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EED CRISPR Activation Plasmid (h2) - sc-401234-ACT-2 from Santa Cruz Biotechnology
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EED Lentiviral Activation Particles (h) - sc-401234-LAC from Santa Cruz Biotechnology
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EED Lentiviral Activation Particles (h2) - sc-401234-LAC-2 from Santa Cruz Biotechnology
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EED siRNA (h) - sc-37823 from Santa Cruz Biotechnology
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EED shRNA Plasmid (h) - sc-37823-SH from Santa Cruz Biotechnology
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EED shRNA (h) Lentiviral Particles - sc-37823-V from Santa Cruz Biotechnology
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EED RNAi - H00008726-R02 from Novus Biologicals
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EED RNAi - H00008726-R01 from Novus Biologicals
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MISSION® esiRNA - EHU062201 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7661 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7661 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06255 from Sigma-Aldrich
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EED siRNA (Human) - CRH5746 from Cohesion Biosciences
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EED - MBS8203092 from MyBioSource
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shRNA set against Human EED (NM_003797.3) - SHH283245 from Creative biogene
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Human EED siRNA - orb266248 from Biorbyt
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EED shRNA Plasmid (Human) - CHH5746 from Cohesion Biosciences
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EED miRNA 3'UTR clone - MiUTR3H-08101 from Creative biogene
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EED miRNA 3'UTR clone - MiUTR3H-08102 from Creative biogene
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