EIF4EBP1

Gene Symbol EIF4EBP1
Entrez Gene 1978
Alt Symbol 4E-BP1, 4EBP1, BP-1, PHAS-I
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 4E binding protein 1
Other Description eIF4E-binding protein 1|eukaryotic translation initiation factor 4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
Swissprots Q6IBN3 Q13541 B2R502 D3DSW8
Accessions CCW44094 EAW63341 EAW63342 Q13541 AB044548 BAB18650 AK312011 BAG34949 BC004459 AAH04459 BC058073 AAH58073 BF964628 BT007162 AAP35826 CR456769 CAG33050 DQ890570 ABM92187 DQ893731 ABM84657 L36055 AAA62269 NM_004095 NP_004086
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. {ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:7935836}.
Top Pathways RNA transport, Choline metabolism in cancer, AMPK signaling pathway, PI3K-Akt signaling pathway, ErbB signaling pathway

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 5123 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 236B4
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9903 (blue), using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) - 3929 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 236B4
Western blot analysis of extracts from HeLa cells, serum-starved and stimulated with insulin (100 nM for 5 minutes), with (+) or without (-) λ phosphatase treatment, using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (upper) and 4E-BP1 (53H11) Rabbit mAb #9644 (lower).

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate) - 7547 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 236B4
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate).

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 2846 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 236B4
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Alexa Fluor ® 488 Conjugate).

4E-BP1 (53H11) Rabbit mAb (Biotinylated) - 8594 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 53H11
Western blot analysis of extracts from MCF7 and HeLa cells using 4E-BP1 (53H11) Rabbit mAb (Biotinylated).

Phospho-4E-BP1 (Ser65) Antibody - 9451 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells using 4E-BP1 Antibody #9644 (lower) and Phospho-4E-BP1 (Ser65) Antibody #9451 (upper). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.

Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb - 9456 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 174A9
Western blot analysis of extracts from 293 cells, lambda phosphatase treated, or treated with 20% FBS, using Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb (upper) or 4E-BP1 Antibody #9452 (lower).

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb - 2855 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 236B4
Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody #2855 (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.

Phospho-4E-BP1 (Thr70) Antibody - 9455 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indicated number of minutes, using Phospho-4E-BP1 (Thr70) Antibody (upper) or 4E-BP1 Antibody #9452 (lower).

Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb - 13396 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7F6I
Western blot analysis of extracts from serum starved HeLa cells, untreated (-), insulin-treated (1 μM, 30 min; +), or insulin and λ phosphatase-treated (+), using Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb (upper), 4E-BP1 (53H11) Rabbit mAb #9644 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb - 13443 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9G1Q
Western blot analysis of extracts from serum starved HeLa cells, untreated (-), insulin-treated (1 μM, 30 min; +), or insulin and λ phosphatase-treated (+), using Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb (upper), 4E-BP1 (53H11) Rabbit mAb #9644 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Phospho-4E-BP1 (Thr37/46) Antibody - 9459 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.

Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb - 4923 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 87D12
Western blot analysis of extracts from COS cells, treated with λ phosphatase or 20% FBS as indicated, using Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb (upper), Phospho-4E-BP1 (Thr37/46) Antibody #9459 (middle), and 4E-BP1 Antibody #9452 (lower).

4E-BP1 (53H11) Rabbit mAb - 9644 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 53H11
Western blot analysis of extracts from various cell lines using 4E-BP1 (53H11) Rabbit mAb.

4E-BP1 Antibody - 9452 from Cell Signaling Technology

Prices $246.00, $596.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using 4E-BP1 Antibody.

Phospho-4E-BP1 (Thr37) Antibody - 9457 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-4E-BP1 (Thr46) Antibody - 9458 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-4E-BP1 (Thr70) II Antibody - 5078 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, serum-starved and treated with 100 nM insulin alone, or with insulin and λ phosphatase, using Phospho-4E-BP1 (Thr70) II Antibody (upper) or 4E-BP1 (53H11) Rabbit mAb #9644 (lower).

4E-BP1 (11G12C11) - sc-81149 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
4E-BP1 (11G12C11): sc-81149. Western blot analysis of 4E-BP1 expression in K-562 (A) and HL-60 (B) whole cell lysates.

4E-BP1 (C-19) - sc-6024 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of His-tagged rat recombinant 4E-BP1 (A-C). Antibodies tested include 4E-BP1 (N-19): sc-6025 (A), 4E-BP1 (C-19): sc-6024 (B) and 4E-BP1 (R-113): sc-6936 (C).

4E-BP1 (D-10) - sc-514073 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
4E-BP1 (D-10): sc-514073. Western blot analysis of 4E-BP1 expression in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-174963 (B) 293T whole cell lysates.

4E-BP1 (N-19) - sc-6025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
4E-BP1 (N-19): sc-6025. Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic localization.

4E-BP1 (P-1) - sc-9977 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
4E-BP1 (P-1): sc-9977. Western blot analysis of 4E-BP1 expression in A-431 (A), K-562 (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.

4E-BP1 (R-113) - sc-6936 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of His-tagged rat recombinant 4E-BP1 (A-C). Antibodies tested include 4E-BP1 (N-19): sc-6025 (A), 4E-BP1 (C-19): sc-6024 (B) and 4E-BP1 (R-113): sc-6936 (C).

4E-BP1/2/3 (H-40) - sc-98602 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
4E-BP1/2/3 (H-40): sc-98602. Western blot analysis of 4E-BP1/2/3 expression in HeLa whole cell lysate.
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