SMAD1

Gene Symbol SMAD1
Entrez Gene 4086
Alt Symbol BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1
Species Human
Gene Type protein-coding
Description SMAD family member 1
Other Description MAD homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|mothers against decapentaplegic homolog 1|transforming growth factor-beta signaling protein 1|transforming growth factor-beta-signaling protein 1
Swissprots A8KAJ0 Q9UFT8 Q15797 Q16636 D3DNZ9
Accessions CAB55898 CBY84968 EAX05037 EAX05038 EAX05039 EAX05040 Q15797 AK096624 AK293055 BAF85744 BC001878 AAH01878 BE644607 BI772689 BI792941 BQ962660 BT007386 AAP36050 BU163870 CB956339 DQ892510 ABM83436 DQ895724 ABM86650 U54826 AAC50493 U57456 AAC50621 U59423 AAB06852 U59912 AAC50790 XM_005262991 XP_005263048 XM_005262992 XP_005263049 XM_005262993 XP_005263050 XM_006714217 XP_006714280 XM_011531961 XP_011530263 XM_011531962 XP_011530264 XM_011531963 XP_011530265 XM_011531964 XP_011530266 NM_001003688 NP_001003688 NM_005900 NP_005891
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. {ECO:0000269|PubMed:12097147}.
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
Tissue Specificity Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Top Pathways TGF-beta signaling pathway, Hippo signaling pathway, Signaling pathways regulating pluripotency of stem cells, Transcriptional misregulation in cancer

Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (PE Conjugate) - 12428 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D5B10
Flow cytometric analysis of HT-1080 cells, untreated (blue) or treated with Human BMP2 #4697 (50 ng/ml, 30 min; green), using Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (PE Conjugate).

Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated) - 9576 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 41D10
Western blot analysis of extracts from untreated or BMP4-treated HT-1080 cells using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated) and detected using Streptavidin-HRP #3999.

Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (ChIP formulated) - 11971 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 immunoprecipitations)
Host Rabbit
Clonality Monoclonal
Clone D5B10
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF7 cells treated with Human BMP2 #4697 (50 ng/ml) for 1 hr and either 5 μl of Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (ChIP formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human ID1 Promoter Primers #5139, human SMAD6 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb - 9516 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 41D10
Western blot analysis of extracts from untreated or BMP-4-treated HeLa or NIH/3T3 cells using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb.

Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb - 13820 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5B10
Western blot analysis of extracts from Hep G2 or MEF cells, untreated (-) or treated with Human BMP2 #4697 (50 ng/ml, 30 min; +), using Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (upper) and Smad1 (D59D7) XP ® Rabbit mAb #6944 (lower).

Phospho-Smad1 (Ser206) Antibody - 9553 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of ACHN and HeLa cells, untreated or UV-treated, using Phospho-Smad1 (Ser206) Antibody (upper), or total Smad1 Antibody #9743 (lower).

Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb - 5753 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D40B7
Western blot analysis of extracts of HeLa cells, untreated or UV-treated (60 mJ/cm 2 for 2 minutes followed by 1.5 hour recovery), using Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb (upper) and Smad1 Antibody #9743 (lower).

Smad1 (D59D7) XP ® Rabbit mAb - 6944 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D59D7
Western blot analysis of extracts from various cell lines using Smad1 (D59D7) XP ® Rabbit mAb.

Smad1 Antibody - 9743 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Smad1 Antibody.

Phospho-Smad3 (Ser423/425)/Smad1 (Ser463/465) Antibody - 9514 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) Antibody - 9511 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Smad1 Antibody - 9512 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HepG2, SK-N-MC, and COS cells, using Smad1 Antibody.

Smad (C-17) - sc-6030 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad (C-17): sc-6030. Western blot analysis of Smad2 expression in non-transfected: sc-117752 (A) and human Smad2 transfected: sc-113452 (B) 293T whole cell lysates.

Smad (H-465) - sc-7153 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Smad (H-465): sc-7153. Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Smad1 (913C1b) - sc-81378 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Smad1 (913C1b): sc-81378 Western Blot analysis of Smad1 expression in F2408 whole cell lysate.

Smad1 (A-4) - sc-7965 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad1 (A-4): sc-7965. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.

Smad1/2/3 (H-2) - sc-7960 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad1/2/3 (H-2): sc-7960. Western blot analysis of Smad1/2/3 expression in Mv 1 Lu (A) and NIH/3T3 (B) whole cell lysates.

Smad1/5 (T-20) - sc-6201 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Smad1 (Ser 465) - sc-101800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Smad1 (Ser 465): sc-101800. Western blot analysis of phosphorylated Smad1 expression in untreated (A) and PMA-treated (B) 293 whole cell lysates.

p-Smad1/5/8 (Ser 463/Ser 465) - sc-12353 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Smad1/5/8 (Ser 463/Ser 465)-R: sc-12353-R. Western blot analysis of Smad1 phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase (sc-200312A) treated (C) HEK293 whole cell lysates.

Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - ab108965 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2290(2)
All lanes : Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965) at 1/1000 dilutionLane 1 : 293T lysateLane 2 : A673 lysateLane 3 : Human heart lysateLane 4 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryLanes 1, 3 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SMAD 1/2/3/5 antibody - ab95455 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMAD 1/5/8/9 antibody - ab13723 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.

Anti-Smad1 (phospho S187) antibody - ab73211 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Smad1 in paraffin-embedded human breast carcinoma tissue using ab73211 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

Anti-Smad1 (phospho S465) antibody - ab97689 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad1 (phospho S465) antibody (ab97689) at 1/500 dilutionLane 1 : HeLa cell extracts, untreatedLane 2 : HeLa cell extracts, treated with PMA
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