Gene Symbol | ALKBH3 |
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Entrez Gene | 221120 |
Alt Symbol | ABH3, DEPC-1, DEPC1, PCA1 |
Species | Human |
Gene Type | protein-coding |
Description | alkB, alkylation repair homolog 3 (E. coli) |
Other Description | alkylated DNA repair protein alkB homolog 3|alpha-ketoglutarate-dependent dioxygenase alkB homolog 3|prostate cancer antigen 1|prostate cancer antigen-1 |
Swissprots | Q3SYI0 Q96BU8 Q6NX57 Q96Q83 A6NDJ1 |
Accessions | ABA27096 CAC38593 CAG17896 EAW68078 EAW68079 Q96Q83 AB042029 BAB70508 AK125602 AK315266 BC015155 AAH15155 BC067257 AAH67257 BC103812 AAI03813 BC103813 AAI03814 BC103814 AAI03815 CD364334 DA685031 U38442 NM_139178 NP_631917 |
Function | Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:22055184}. |
Subcellular Location | Cytoplasm. Nucleus. |
Tissue Specificity | Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:17979886}. |
ALKBH3 CRISPR/Cas9 KO Plasmid (h) - sc-406896 from Santa Cruz Biotechnology
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ALKBH3 HDR Plasmid (h) - sc-406896-HDR from Santa Cruz Biotechnology
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ALKBH3 Double Nickase Plasmid (h) - sc-406896-NIC from Santa Cruz Biotechnology
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ALKBH3 Double Nickase Plasmid (h2) - sc-406896-NIC-2 from Santa Cruz Biotechnology
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ALKBH3 CRISPR Activation Plasmid (h) - sc-406896-ACT from Santa Cruz Biotechnology
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ALKBH3 CRISPR Activation Plasmid (h2) - sc-406896-ACT-2 from Santa Cruz Biotechnology
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ALKBH3 Lentiviral Activation Particles (h) - sc-406896-LAC from Santa Cruz Biotechnology
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ALKBH3 Lentiviral Activation Particles (h2) - sc-406896-LAC-2 from Santa Cruz Biotechnology
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ALKBH3 siRNA (h) - sc-96711 from Santa Cruz Biotechnology
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ALKBH3 shRNA Plasmid (h) - sc-96711-SH from Santa Cruz Biotechnology
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ALKBH3 shRNA (h) Lentiviral Particles - sc-96711-V from Santa Cruz Biotechnology
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ALKBH3 RNAi - H00221120-R01 from Novus Biologicals
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MISSION® esiRNA - EHU064581 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3068 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3068 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR12048 from Sigma-Aldrich
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ALKBH3 siRNA (Human) - CRJ6491 from Cohesion Biosciences
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ALKBH3 - MBS828019 from MyBioSource
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ALKBH3 HDR Plasmid (h2) - sc-406896-HDR-2 from Santa Cruz Biotechnology
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ALKBH3 CRISPR/Cas9 KO Plasmid (h2) - sc-406896-KO-2 from Santa Cruz Biotechnology
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shRNA set against Human ALKBH3 (NM_139178.3) - SHH235234 from Creative biogene
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ALKBH3 shRNA Plasmid (Human) - CHJ6491 from Cohesion Biosciences
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ALKBH3 miRNA 3'UTR clone - MiUTR3H-11346 from Creative biogene
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