PPP1R15A

Gene Symbol PPP1R15A
Entrez Gene 23645
Alt Symbol GADD34
Species Human
Gene Type protein-coding
Description protein phosphatase 1, regulatory subunit 15A
Other Description growth arrest and DNA damage-inducible protein GADD34|growth arrest and DNA-damage-inducible 34|myeloid differentiation primary response protein MyD116 homolog|protein phosphatase 1 regulatory subunit 15A|protein phosphatase 1, regulatory (inhibitor) subunit 15A
Swissprots O75807 Q9NVU6 Q6IA96 B4DKQ3
Accessions CBX47609 EAW52409 O75807 AK001361 BAA91649 AK225632 AK296668 BAG59265 AK297615 BAG59994 AK298765 BAG60907 AK311366 BC003067 AAH03067 CR457259 CAG33540 DA484808 DQ891855 ABM82781 EU176641 ABW03442 U83981 AAC25631 NM_014330 NP_055145
Function Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. {ECO:0000269|PubMed:11564868, ECO:0000269|PubMed:12556489, ECO:0000269|PubMed:14635196, ECO:0000269|PubMed:14718519, ECO:0000269|PubMed:8139541}.
Subcellular Location Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side {ECO:0000269|PubMed:12556489, ECO:0000269|PubMed:21518769}. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side {ECO:0000269|PubMed:21518769}. Note=Associates with membranes via an N-terminal amphipathic intramembrane region. {ECO:0000269|PubMed:21518769}.
Top Pathways Protein processing in endoplasmic reticulum

GADD 34 (B-10) - sc-373815 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GADD 34 (B-10): sc-373815. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

GADD 34 (D-8) - sc-46661 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of control (A,C) and GADD 34 transfected (B,D) COS cells. Antibodies tested include GADD 34 (D-8): sc-46661 (A,B) and GADD 34 (H-193): sc-8327 (C,D).

GADD 34 (C-20) - sc-794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GADD 34 (C-20): sc-794. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and Islets of Langerhans.

GADD 34 (H-193) - sc-8327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of control (A,C) and GADD 34 transfected (B,D) COS cells. Antibodies tested include GADD 34 (D-8): sc-46661 (A,B) and GADD 34 (H-193): sc-8327 (C,D).

GADD 34 (S-20) - sc-824 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GADD 34 (S-20): sc-824. Western blot analysis of human recombinant GADD34 fusion protein.

Anti-GADD34 antibody - ab9869 from Abcam

Prices $382.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-GADD34 antibody (ab9869) at 0.1 µg/ml + HEPG2 cell lysate at 35 µg

Anti-GADD34 antibody - ab126075 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab126075 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126075, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GADD34 antibody - ab175355 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 2 : Anti-GADD34 antibody (ab175355) at 1/250 dilutionLanes 3 & 5 : Anti-GADD34 antibody (ab175355) at 1/500 dilutionLane 4 : Anti-GADD34 antibody (ab175355) at 1/1000 dilutionLane 2 : HeLa cell lysateLane 3 : HeLa cell lysateLane 4 : HeLa cell lysateLane 5 : HeLa cell lysate with neutralizing peptideLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG-HRP secondary antibodydeveloped using the ECL technique

Anti-GADD34 antibody - ab125464 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GADD34 antibody (ab125464) at 1 µg/ml + Human fetal heart lysate at 10 µg

Anti-PPP1R15A / GADD34 Antibody (aa664-674) - LS-C55303 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.1 ug/ml) staining of HEPG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PPP1R15A / GADD34 Antibody (aa116-165) - LS-C146499 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PPP1R15A / GADD34 antibody LS-C146499 Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.

Anti-PPP1R15A / GADD34 Antibody (clone 2B11) - LS-C337630 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B11
Anti-PPP1R15A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A.

Anti-PPP1R15A / GADD34 Antibody (clone 1E1) - LS-C173778 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E1
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPP1R15A mouse monoclonal antibody.

Anti-PPP1R15A / GADD34 Antibody (clone 3D12) - LS-C173753 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3D12
IHC of paraffin-embedded Human tonsil using anti-PPP1R15A mouse monoclonal antibody.

Anti-PPP1R15A / GADD34 Antibody (clone 4F1) - LS-C173755 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4F1
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1R15A mouse monoclonal antibody.

GADD34 Antibody (3D12) - NBP2-01787 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3D12
Western Blot: GADD34 Antibody (3D12) [NBP2-01787] Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

GADD34 Antibody - NB100-778 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: GADD34 Antibody [NB100-778] - (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GADD34 Antibody - NBP2-16575 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: GADD34 Antibody [NBP2-16575] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.

GADD34 Antibody [FITC] - NBP2-11495 from Novus Biologicals

Host Rabbit
Clonality Polyclonal
Western Blot: GADD34 Antibody [FITC] [NBP2-11495] - Dilution 1:1000, apparent MW is 75 kDa

GADD34 Polyclonal Antibody - bs-10287R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-GADD34 Polyclonal Antibody, Unconjugated (bs-10287R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-PPP1R15A/GADD34 antibody produced in goat - SAB2500814 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PPP1R15A / GADD34: <B>Cat. No. SAB2500814</B>: (0.1 μg/mL) staining of HEPG2 cell lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PPP1R15A antibody produced in rabbit - HPA020240 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PPP1R15A: <B>Cat. No. HPA020240</B>: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in both exocrine glandular cells and islet cells.

Anti-PPP1R15A antibody produced in rabbit - SAB2701237 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PPP1R15A: <B>Cat. No. SAB2701237</B>: Western Blot analysis of PPP1R15A in extracts from <B>Lanes</B><BR/>A. 293T using <B>Cat. No. SAB2701237</B> at a 1:1000 dilution.

PPP1R15A antibody - N-terminal region (ARP63717_P050) - ARP63717_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PPP1R15A--GADD34-Antibody - AF1853a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF1853a (0.1 µg/ml) staining of HEPG2 cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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