CLCN5

Gene Symbol CLCN5
Entrez Gene 1184
Alt Symbol CLC5, CLCK2, ClC-5, DENTS, NPHL1, NPHL2, XLRH, XRN, hCIC-K2
Species Human
Gene Type protein-coding
Description chloride channel, voltage-sensitive 5
Other Description H(+)/Cl(-) exchange transporter 5|chloride transporter ClC-5|voltage-gated chloride ion channel CLCN5
Swissprots Q5JQD5 B3KPN6 P51795 A1L475 Q7RTN8
Accessions EAW89929 P51795 AA743724 AK021494 AK025562 AK056560 BAG51748 AK092049 BAG52474 AK290940 BAF83629 AK308089 BC051691 BC130429 AAI30430 BC130431 AAI30432 BF513571 BK000969 DAA01544 BX950885 DA652242 DA737534 HQ258649 ADR83400 X81836 CAA57430 X91906 CAA63000 XM_011543888 XP_011542190 XM_011543889 XP_011542191 NM_000084 NP_000075 NM_001127898 NP_001121370 NM_001127899 NP_001121371 NM_001272102 NP_001259031 NM_001282163 NP_001269092
Function Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.
Subcellular Location Golgi apparatus membrane {ECO:0000269|PubMed:19019917}; Multi-pass membrane protein {ECO:0000269|PubMed:19019917}. Endosome membrane {ECO:0000269|PubMed:19019917}; Multi-pass membrane protein {ECO:0000269|PubMed:19019917}. Cell membrane {ECO:0000269|PubMed:19019917}; Multi-pass membrane protein {ECO:0000269|PubMed:19019917}.
Tissue Specificity Kidney. Moderately expressed in aortic vascular smooth muscle and endothelial cells, and at a slightly higher level in the coronary vascular smooth muscle. {ECO:0000269|PubMed:10198195}.

CLC-5 (D-17) - sc-22373 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Chloride Channel 5 antibody - ab104752 from Abcam

Host Rabbit
Clonality Polyclonal
ab104752 stained A498 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104752 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Chloride Channel 5 antibody - ab97482 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Chloride Channel 5 antibody (ab97482) at 1/500 dilution + A431 whole cell lysate at 30 µg

Anti-Chloride Channel 5 antibody [N237/24] - ab174937 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone N237/24
IHC image of Chloride Channel 5 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab174937, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Chloride Channel 5 antibody - C-terminal - ab188503 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chloride Channel 5 antibody - C-terminal (ab188503) at 1/500 dilutionLane 1 : SW480 cell extractLane 2 : U937 cell extractLane 3 : HepG2 cell extractLane 4 : A673 cell extractLane 5 : THP1 cell extractLane 6 : H460 cell extractLane 7 : Mouse kidney extractLane 8 : Mouse ovary extractLane 9 : Mouse brain extract

Anti-Chloride Channel 5 antibody - N-terminal - ab156155 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Chloride Channel 5 antibody - N-terminal (ab156155) at 1/100 dilution + K562 cell line lysate at 35 µg

Anti-ClC-5 / CLCN5 Antibody (C-Terminus) - LS-C6131 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (C-Terminus) - LS-C6132 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa15-41, AP) - LS-C254932 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa15-41, APC) - LS-C254935 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa15-41, FITC) - LS-C254936 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa15-41, Biotin) - LS-C254934 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa15-41) - LS-C320414 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa15-41, Azide-free, HRP) - LS-C254938 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa15-41, PE) - LS-C254937 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody - LS-C341662 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-5 / CLCN5 Antibody (aa521-570) - LS-C135021 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CLCN5 / CLC5 antibody LS-C135021 Western blot of Mouse Brain lysate.

Anti-ClC-5 / CLCN5 Antibody (aa62-393) - LS-C185592 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. ClC-5 / CLCN5 antibody diluted at 1:500

Anti-ClC-5 / CLCN5 Antibody (aa15-41) - LS-C157646 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CLCN5 Antibody western blot of K562 cell line lysates (35 ug/lane). The CLCN5 antibody detected the CLCN5 protein (arrow).

Anti-ClC-5 / CLCN5 Antibody - LS-C334230 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human rectal cancer using CLCN5 antibody at dilution of 1:200 (x400 lens)

CLCN5 Antibody - NBP1-69123 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CLCN5 Antibody [NBP1-69123] - Rat Brain Antibody Dilution: 1.0 ug/ml

CLCN5 Antibody - NBP1-70374 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CLCN5 Antibody - NBP1-32302 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CLCN5 Antibody - NBP1-84451 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: CLCN5 Antibody [NBP1-84451] - Staining of human cerebral cortex shows moderate granular positivity in neuronal cells.

CLCN5 Antibody - H00001184-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
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