PGAP3

Gene Symbol PGAP3
Entrez Gene 93210
Alt Symbol AGLA546, CAB2, PERLD1, PP1498, hCOS16
Species Human
Gene Type protein-coding
Description post-GPI attachment to proteins 3
Other Description COS16 homolog|gene coamplified with ERBB2 protein|per1-like domain containing 1|post-GPI attachment to proteins factor 3
Swissprots Q86Z03 Q96FM1 Q8NBJ8 B4DGK7
Accessions EAW60589 EAW60590 EAW60591 EAW60592 EAW60593 EAW60594 Q96FM1 AB088396 BAC55580 AF217980 AAG17223 AK057911 AK075473 AK075474 BAC11642 AK289612 BAF82301 AK294639 BAG57818 AK301106 BAG62705 AK314469 AL833198 AY358437 AAQ88803 BC010652 AAH10652 BG472165 DC389056 XM_011525480 XP_011523782 XM_011525481 XP_011523783 XR_934601 NM_001291726 NP_001278655 NM_001291728 NP_001278657 NM_001291730 NP_001278659 NM_001291732 NP_001278661 NM_001291733 NP_001278662 NM_033419 NP_219487
Function Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable). {ECO:0000305|PubMed:17021251}.
Subcellular Location Golgi apparatus membrane {ECO:0000305|PubMed:12460457}; Multi-pass membrane protein {ECO:0000305|PubMed:12460457}. Endoplasmic reticulum membrane {ECO:0000305|PubMed:12460457}; Multi-pass membrane protein {ECO:0000305|PubMed:12460457}. Note=Mainly localizes to Golgi apparatus. {ECO:0000305}.
Tissue Specificity Ubiquitously expressed, with highest levels in thyroid and placenta. {ECO:0000269|PubMed:12460457}.

PERLD1 (N-13) - sc-161154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PERLD1 (N-13): sc-161154. Western blot analysis of PERLD1 expression in THP-1 (A), Jurkat (B) and K-562 (C) whole cell lysates.

Anti-PGAP3 antibody - ab129264 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab129264 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129264 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PGAP3 antibody - ab81368 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PGAP3 antibody (ab81368) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PGAP3 antibody - ab89711 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PGAP3 antibody (ab89711) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP-1 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PGAP3 antibody [EPR14380] - ab182569 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14380
All lanes : Anti-PGAP3 antibody [EPR14380] (ab182569) at 1/5000 dilutionLane 1 : Human thyroid tissue lysateLane 2 : Human placenta tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

Anti-PGAP3 / PERLD1 Antibody (aa140-169, AP) - LS-C238941 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PGAP3 / PERLD1 Antibody (aa140-169, APC) - LS-C238942 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PGAP3 / PERLD1 Antibody (aa140-169, Biotin) - LS-C238943 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PGAP3 / PERLD1 Antibody (aa140-169, FITC) - LS-C238944 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PGAP3 / PERLD1 Antibody (aa140-169, PE) - LS-C238945 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PGAP3 / PERLD1 Antibody (aa140-169, Azide-free, HRP) - LS-C248454 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PGAP3 / PERLD1 Antibody (aa140-169) - LS-C323961 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PGAP3 / PERLD1 Antibody (N-Terminus) - LS-C82584 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PGAP3 / PERLD1 Antibody (aa71-120) - LS-C111257 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PGAP3 / PERLD1 antibody LS-C111257 Western blot of THP-1 cell lysate.

Anti-PGAP3 / PERLD1 Antibody (aa141-169) - LS-C163547 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PGAP3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

PGAP3 Antibody - NBP1-86692 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: PGAP3 Antibody [NBP1-86692] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

PGAP3 Antibody - 21670002 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ELISA: PGAP3 Antibody [21670002]

PGAP3 Antibody - NBP1-69334 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PGAP3 Antibody [NBP1-69334] - This Anti-PERLD1 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml.

PGAP3 Antibody - NBP1-69622 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PGAP3 Antibody [NBP1-69622] - This Anti-PERLD1 antibody was used in Western Blot of THP-1 tissue lysate at a concentration of 1ug/ml.

ANTI-PGAP3 (CENTER) antibody produced in rabbit - SAB1306243 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PGAP3 (Center): <B>Cat. No. SAB1306243</B>: PGAP3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35 μg/lane).

Anti-PGAP3 antibody produced in rabbit - HPA016591 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PERLD1: <B>Cat. No. HPA016591</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human duodenum shows cytoplasmic positivity in glandular cells.

Anti-PERLD1, (N-terminal) antibody produced in rabbit - SAB2103601 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PERLD1, antibody produced in rabbit: <B>Cat. No. SAB2103601</B>: Immunoblotting analysis of PERLD1 in THP-1 cell lysate with PERLD1 anitbody at 1 μg/mL.

PERLD1 antibody - N-terminal region (ARP50188_P050) - ARP50188_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PERLD1 antibody - N-terminal region (ARP50189_P050) - ARP50189_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PGAP3-Antibody-Center - AP13237c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
PGAP3 Antibody (Center) (Cat. #AP13237c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PGAP3 antibody detected the PGAP3 protein (arrow).
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