PHLDA3

Gene Symbol PHLDA3
Entrez Gene 23612
Alt Symbol TIH1
Species Human
Gene Type protein-coding
Description pleckstrin homology-like domain, family A, member 3
Other Description TDAG51/Ipl homolog 1|pleckstrin homology-like domain family A member 3|pleckstrin homology-like domain, family A, member 2
Swissprots Q53HD6 Q8NBW9 B2R5A4 Q9Y5J5
Accessions EAW91369 Q9Y5J5 AF151100 AAD42081 AI083980 AI188803 AI624156 AI678238 AI870979 AK075179 BAC11454 AK222644 BAD96364 AK312115 BAG35051 AW194162 AW513769 BC014390 AAH14390 BC068273 AAH68273 BE778799 BE892338 BG990425 BM996951 BQ000725 BX090314 CA406398 CN356697 JF432856 ADZ16073 N94465 W30744 NM_012396 NP_036528 NR_073080
Function p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor. {ECO:0000269|PubMed:19203586}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:19203586}. Membrane {ECO:0000269|PubMed:19203586}; Peripheral membrane protein {ECO:0000269|PubMed:19203586}.
Tissue Specificity Widely expressed with lowest expression in liver and spleen. {ECO:0000269|PubMed:10594239}.

PHLDA3 Antibody - 4294 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A172 and MCF7 cells using PHLDA3 Antibody.

PHLDA3 (H-54) - sc-98599 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

PHLDA3 (T-16) - sc-324202 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PHLDA3 antibody - ab22822 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Lanes 1 - 2 : Anti-PHLDA3 antibody (ab22822) at 1 µg/mlLane 3 : Anti-myc tag antibody at 1/1000 dilutionLane 1 : HEK293 lysate overexpressing Human PHLDA3 in RIPA buffer at 10 µgLane 2 : Mock-transfected HEK293Lane 3 : HEK293 lysate overexpressing Human PHLDA3 in RIPA buffer at 10 µgdeveloped using the ECL technique

Anti-PHLDA3 antibody - ab196757 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PHLDA3 with ab196757 at 1/50 dilution (left) and synthesized peptide (right).

Anti-PHLDA3 antibody - ab104766 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PHLDA3 antibody (ab104766) at 1 µg/ml + 293T cell lysate at 10 µg

Anti-PHLDA3 antibody [4B6] - ab81464 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4B6

Anti-PHLDA3 Antibody (aa115-127) - LS-C55274 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-PHLDA3 Antibody (aa21-70) - LS-C119587 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human breast carcinoma tissue, using PHLDA3 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-PHLDA3 Antibody (aa38-87) - LS-C81956 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.

PHLDA3 Antibody - NBP1-56772 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PHLDA3 Antibody [NBP1-56772] - 293T cells lysate, concentration 0.2-1 ug/ml.

PHLDA3 Antibody - NBP1-80683 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PHLDA3 Antibody [NBP1-80683] - Analysis in control (vector only transfected HEK293T lysate) and PHLDA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Anti-PHLDA3 antibody produced in rabbit - SAB4501014 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PHLDA3: <B>Cat. No. SAB4501014</B>: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-PHLDA3 antibody. Cells on the left were treated with the Anti-PHLDA3 antibody. Cells on the right (negative control) were treated with both Anti-PHLDA3 antibody and the synthesized immunogen peptide.

Anti-PHLDA3 antibody produced in rabbit - HPA027601 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PHLDA3: <B>Cat. No. HPA027601</B>: Immunohistochemical staining of human small intestine shows generally weak positivity in glandular cells but goblet cells were stronger stained.

Anti-PHLDA3 (C-terminal) antibody produced in goat - SAB2501510 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PHLDA3 (C-terminal) <B>Cat. No. SAB2501510</B>: HEK293 lysate (10 μg protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with SAB2501510 (1 μg/mL) in Lane A and probed with Anti-MYC Tag (1/1,000) in Lane C. Mock-transfected HEK293 probed with SAB2501510 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-PHLDA3 antibody produced in rabbit - SAB2101799 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PHLDA3: <B>Cat. No.SAB2101799</B>: PHLDA3 in 293T cell lysate with PHLDA3 antibody at 1.0 μg/mL.

PHLDA3 antibody - N-terminal region (ARP54891_P050) - ARP54891_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PHLDA3--TIH1-Antibody-C-Term - AF2383a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with AF2383a (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AF2383a (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

PHLDA3 Antibody (OAAF03064) - OAAF03064 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Goat Anti-PHLDA3 / TIH1 Antibody (OAEB00986) - OAEB00986 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

PHLDA3 antibody - 70R-4228 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

PHLDA3 Antibody - abx014056 from Abbexa

Host Rabbit
Clonality Polyclonal

PHLDA3 (C-term) antibody - AP23766PU-N from Acris Antibodies

Prices $310.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with AP23766PU-N (1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP23766PU-N (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-PHLDA3 Antibody - AC21-0471 from Abcore

Prices $359.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-PHLDA3 Antibody (AMCA) - AC21-0471-01 from Abcore

Prices $495.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
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