| Gene Symbol | SPOP |
|---|---|
| Entrez Gene | 8405 |
| Alt Symbol | BTBD32, TEF2 |
| Species | Human |
| Gene Type | protein-coding |
| Description | speckle-type POZ protein |
| Other Description | HIB homolog 1|roadkill homolog 1 |
| Swissprots | B2R6S3 Q53HJ1 D3DTW7 O43791 |
| Accessions | CAC39513 EAW94671 EAW94672 EAW94673 EAW94674 EAW94675 O43791 AJ000644 CAA04199 AK123385 AK222589 BAD96309 AK293045 BAF85734 AK293587 BAG57055 AK300616 BAG62309 AK303268 BAG64350 AK312691 BAG35570 AK316366 BAH14737 AM392588 CAL37466 AM392643 CAL37521 AM392731 CAL37609 AM393008 CAL37886 AM393567 CAL38444 AM393826 CAL38701 AW449169 BC001269 AAH01269 BC003385 AAH03385 BM693397 BQ226031 BX282325 CB992730 W84567 XM_005257723 XP_005257780 XM_005257724 XP_005257781 NM_001007226 NP_001007227 NM_001007227 NP_001007228 NM_001007228 NP_001007229 NM_001007229 NP_001007230 NM_001007230 NP_001007231 NM_003563 NP_003554 |
| Function | Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. {ECO:0000269|PubMed:14528312, ECO:0000269|PubMed:15897469, ECO:0000269|PubMed:16524876, ECO:0000269|PubMed:19818708, ECO:0000269|PubMed:22085717, ECO:0000269|PubMed:22632832}. |
| Subcellular Location | Nucleus. Nucleus speckle. |
| Tissue Specificity | Widely expressed. {ECO:0000269|PubMed:9414087}. |
SPOP CRISPR/Cas9 KO Plasmid (h) - sc-401290 from Santa Cruz Biotechnology
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SPOP HDR Plasmid (h) - sc-401290-HDR from Santa Cruz Biotechnology
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SPOP Double Nickase Plasmid (h) - sc-401290-NIC from Santa Cruz Biotechnology
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SPOP Double Nickase Plasmid (h2) - sc-401290-NIC-2 from Santa Cruz Biotechnology
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SPOP CRISPR Activation Plasmid (h) - sc-401290-ACT from Santa Cruz Biotechnology
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SPOP CRISPR Activation Plasmid (h2) - sc-401290-ACT-2 from Santa Cruz Biotechnology
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SPOP Lentiviral Activation Particles (h) - sc-401290-LAC from Santa Cruz Biotechnology
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SPOP Lentiviral Activation Particles (h2) - sc-401290-LAC-2 from Santa Cruz Biotechnology
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SPOP siRNA (h) - sc-63056 from Santa Cruz Biotechnology
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SPOP shRNA Plasmid (h) - sc-63056-SH from Santa Cruz Biotechnology
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SPOP shRNA (h) Lentiviral Particles - sc-63056-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU056151 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20507 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20507 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR09489 from Sigma-Aldrich
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SPOP siRNA (Human) - CRH5508 from Cohesion Biosciences
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SPOP - MBS8223615 from MyBioSource
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shRNA set against Human SPOP(NM_001007226.1) - SHL030106 from Creative biogene
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Human SPOP siRNA - orb266010 from Biorbyt
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SPOP shRNA Plasmid (Human) - CHH5508 from Cohesion Biosciences
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SPOP miRNA 3'UTR clone - MiUTR1H-09861 from Creative biogene
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SPOP miRNA 3'UTR clone - MiUTR3H-05179 from Creative biogene
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SPOP miRNA 3'UTR clone - MiUTR3H-05180 from Creative biogene
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SPOP miRNA 3'UTR clone - MiUTR3H-05181 from Creative biogene
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SPOP miRNA 3'UTR clone - MiUTR3H-05182 from Creative biogene
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