SPOP

Gene Symbol SPOP
Entrez Gene 8405
Alt Symbol BTBD32, TEF2
Species Human
Gene Type protein-coding
Description speckle-type POZ protein
Other Description HIB homolog 1|roadkill homolog 1
Swissprots B2R6S3 Q53HJ1 D3DTW7 O43791
Accessions CAC39513 EAW94671 EAW94672 EAW94673 EAW94674 EAW94675 O43791 AJ000644 CAA04199 AK123385 AK222589 BAD96309 AK293045 BAF85734 AK293587 BAG57055 AK300616 BAG62309 AK303268 BAG64350 AK312691 BAG35570 AK316366 BAH14737 AM392588 CAL37466 AM392643 CAL37521 AM392731 CAL37609 AM393008 CAL37886 AM393567 CAL38444 AM393826 CAL38701 AW449169 BC001269 AAH01269 BC003385 AAH03385 BM693397 BQ226031 BX282325 CB992730 W84567 XM_005257723 XP_005257780 XM_005257724 XP_005257781 NM_001007226 NP_001007227 NM_001007227 NP_001007228 NM_001007228 NP_001007229 NM_001007229 NP_001007230 NM_001007230 NP_001007231 NM_003563 NP_003554
Function Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. {ECO:0000269|PubMed:14528312, ECO:0000269|PubMed:15897469, ECO:0000269|PubMed:16524876, ECO:0000269|PubMed:19818708, ECO:0000269|PubMed:22085717, ECO:0000269|PubMed:22632832}.
Subcellular Location Nucleus. Nucleus speckle.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:9414087}.

SPOP (B-8) - sc-377206 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SPOP (B-8): sc-377206. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and membrane localization.

SPOP (K-13) - sc-66651 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SPOP (C-14) - sc-66649 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SPOP (C-14): sc-66649. Western blot analysis of SPOP expression in non-transfected: sc-110760 (A) and human SPOP transfected: sc-110775 (B) 293 whole cell lysates.

Anti-SPOP antibody [EPR15771] - C-terminal - ab192233 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15771
All lanes : Anti-SPOP antibody [EPR15771] - C-terminal (ab192233) at 1/1000 dilutionLane 1 : PC3 cell lysateLane 2 : HCT116 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-SPOP antibody - N-terminal - ab138630 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SPOP antibody - N-terminal (ab138630) at 1/100 dilution + WiDr cell lysate at 35 µg

Anti-SPOP Antibody (aa86-115) - LS-C157080 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SPOP Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPOP antibody detected the SPOP protein (arrow).

Anti-SPOP Antibody - LS-C109401 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate). A: 293T. 10% SDS PAGE. SPOP antibody diluted at 1:1000.

Anti-SPOP Antibody (aa74-107) - LS-C344692 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from mouse brain and Zebrafish tissue lysate (from left to right) with (DANRE) spop Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Anti-SPOP Antibody (aa86-115, AP) - LS-C241200 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP Antibody (aa86-115, APC) - LS-C241201 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP Antibody (aa86-115, Biotin) - LS-C241202 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP Antibody (aa86-115, FITC) - LS-C241203 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP Antibody (aa86-115, PE) - LS-C241205 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP Antibody (aa86-115, Azide-free, HRP) - LS-C248240 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP Antibody (aa85-115) - LS-C325345 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP Antibody (C-Terminus) - LS-C81545 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-SPOP Antibody (clone 3E2) - LS-C198555 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E2
Detection limit for recombinant GST tagged SPOP is approximately 0.3 ng/ml as a capture antibody.

SPOP Antibody - H00008405-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

SPOP Antibody - NBP2-13372 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: SPOP Antibody [NBP2-13372] - Staining of human urinary bladder shows strong granular cytoplasmic positivity in urothelial cells.

SPOP Antibody - NBP1-33090 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SPOP Antibody [NBP1-33090] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.

SPOP Antibody - NBP2-20477 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SPOP Antibody [NBP2-20477] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.

SPOP Antibody (3E2.) - H00008405-M04 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E2.

SPOP Antibody - H00008405-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: SPOP Antibody [H00008405-D01P] - Analysis of SPOP expression in transfected 293T cell line by SPOP polyclonal antibody.Lane 1: SPOP transfected lysate(42.10 KDa).Lane 2: Non-transfected lysate.

SPOP Polyclonal Antibody - bs-7134R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SPOP antibody produced in rabbit - SAB1411498 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SPOP: <B>Cat. No. SAB1411498</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~42.1 kDa using SPOP transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.
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