Gene Symbol | PSMD10 |
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Entrez Gene | 5716 |
Alt Symbol | dJ889N15.2, p28, p28(GANK) |
Species | Human |
Gene Type | protein-coding |
Description | proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 |
Other Description | 26S proteasome non-ATPase regulatory subunit 10|26S proteasome regulatory subunit p28|ankyrin repeat protein|gankyrin|hepatocellular carcinoma-associated protein p28-II |
Swissprots | Q8IZK9 Q5U0B2 O75832 |
Accessions | CAV33747 EAX02697 EAX02698 O75832 AB009619 BAA33215 AK058108 AK295996 BAG58771 AK309326 AY057056 AAL25260 BC011960 AAH11960 BI114640 BQ217943 BT019689 AAV38495 D83197 BAA34594 GQ129344 ACT64478 GQ129345 ACT64479 R15438 NM_002814 NP_002805 NM_170750 NP_736606 |
Function | Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:18040287}. Nucleus {ECO:0000269|PubMed:18040287}. |
Tissue Specificity | Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level). {ECO:0000269|PubMed:10613832, ECO:0000269|PubMed:20628200}. |
gankyrin CRISPR/Cas9 KO Plasmid (h) - sc-402164 from Santa Cruz Biotechnology
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gankyrin HDR Plasmid (h) - sc-402164-HDR from Santa Cruz Biotechnology
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gankyrin Double Nickase Plasmid (h) - sc-402164-NIC from Santa Cruz Biotechnology
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gankyrin Double Nickase Plasmid (h2) - sc-402164-NIC-2 from Santa Cruz Biotechnology
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gankyrin CRISPR Activation Plasmid (h) - sc-402164-ACT from Santa Cruz Biotechnology
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gankyrin CRISPR Activation Plasmid (h2) - sc-402164-ACT-2 from Santa Cruz Biotechnology
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gankyrin Lentiviral Activation Particles (h) - sc-402164-LAC from Santa Cruz Biotechnology
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gankyrin Lentiviral Activation Particles (h2) - sc-402164-LAC-2 from Santa Cruz Biotechnology
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gankyrin siRNA (h) - sc-72186 from Santa Cruz Biotechnology
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gankyrin shRNA Plasmid (h) - sc-72186-SH from Santa Cruz Biotechnology
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gankyrin shRNA (h) Lentiviral Particles - sc-72186-V from Santa Cruz Biotechnology
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Gankyrin RNAi - H00005716-R01 from Novus Biologicals
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MISSION® esiRNA - EHU133991 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17615 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17615 from Sigma-Aldrich
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PSMD10 siRNA (Human) - CRH3861 from Cohesion Biosciences
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Gankyrin RNAi - H00005716-R02 from Novus Biologicals
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shRNA set against Human PSMD10 (NM_170750.2) - SHH385363 from Creative biogene
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Human PSMD10 siRNA - orb264363 from Biorbyt
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PSMD10 shRNA Plasmid (Human) - CHH3861 from Cohesion Biosciences
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PSMD10 miRNA 3'UTR clone - MiUTR3H-06007 from Creative biogene
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PSMD10 miRNA 3'UTR clone - MiUTR3H-06008 from Creative biogene
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